几种旋尾目寄生线虫的研究

在W.Yorke与P.A.Maplestone(1926)的著作里,旋尾总科(Spiruroidea Raillietet Henry,1915)和丝虫总科(Filarioidea Weinland,1858;Stiles,1907)是两个独立的总科。Chitwood(1933),与(1940)等把上述两类和驼形类(CamallanataChitwood,1936)合并成为旋尾目(Spirurida Chitwood,1933)。     

A Lipidomic Study of the Effects of N-methyl-N′-nitro-N-nitrosoguanidine on Sphingomyelin Metabolism

Systems biology is a new and rapidly developing research area in which,by quantitativelydescribing the interaction among all the individual components of a cell,a systems-level understanding of abiological response can be achieved.Therefore,it requires high-throughput measurement technologies forbiological molecules,such as genomic and proteomic approaches for DNA/RNA and protein,respectively.Recently,a new concept,lipidomics,which utilizes the mass spectrometry(MS)method for lipid analysis,has been proposed.Using this lipidomic approach,the effects of N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)on sphingomyelin metabolism,a major class of sphingolipids,were evaluated.Sphingomyelin moleculeswere extracted from cells and analyzed by matrix-assisted laser desorption ionization-time of flight MS.Itwas found that MNNG induced profound changes in sphingomyelin metabolism,including the appearance ofsome new sphingomyelin species and the disappearance of some others,and the concentrations of severalsphmgomyelin species also changed.This was accompanied by the redistribution of acid sphingomyelinase(ASM),a key player in sphingomyelin metabolism.On the other hand,imipramine,an inhibitor of ASM,caused the accumulation of sphingomyelin.It also prevented some of the effects of MNNG,as well as theredistribution of ASM.Taken together,these data suggested that the lipidomic approach is highly effectivefor the systematic analysis of cellular lipids metabolism.     

加强科研管理工作,营造科研创新环境

随着我国科研工作的发展和科技体制改革的不断深化,科学研究领域的竞争也日益激烈。加强医院科研管理,强化科技意识,提高管理水平,对推动医学科学技术的发展具有十分重要的意义。近年来,我院深化改革,走科技兴院的道路,制定一系列科研奖励政策,不断充实完善基础研究设施建设,加     

人胰岛素突变体GIRR的分离纯化及性质研究

构建人胰岛素原突变体GIRR(A2 1Asn→Gly,B1 7Leu→Ile ,B31Arg ,B32Arg)基因的原核融合表达载体并进行表达和纯化 ,进一步对其性质进行研究。将人胰岛素原突变体基因重组到pTXB1融合表达载体上 ,在E .coliBL2 1 (DE3)中得到高效表达。表达产物经几丁质亲和柱层析分离以及胰蛋白酶和羧肽酶B的酶促转化等步骤 ,得到纯的人胰岛素突变体GIRR ,其纯度经HPLC鉴定达到 95 % ,氨基酸组成和设计相符 ,其受体结合活性是猪胰岛素的 1 0 5 % ,生物活性为 2 6.8IU/mg。其血浆半衰期为 2 0分钟 ,比标准猪胰岛素提高 3倍多。结果提示本突变体具有更长的半衰期 ,为长效胰岛素的制备提供了新的思路。     

肝细胞特异性基因治疗载体的构建及活性研究

以细胞膜穿透肽 (Penetratin)基因为主要模板序列 ,融合肝细胞膜受体结合蛋白的DNA序列 ,设计一段肝细胞基因治疗载体的特异基因序列 ,构建并表达了肝细胞特异性载体体系及 3种对照体系。然后进行肝细胞的穿膜实验 ,细胞荧光显色结果提示肝细胞特异性载体体系可以有效地介导外源蛋白EGFP的基因进入肝细胞 ,并在肝细胞内表达。结论提示 ,所构建的载体体系有可能为肝细胞基因治疗提供一种新型的非病毒基因治疗载体。     

Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1)

INTroDUCTIONP-, E- and L-selectin are C-type lectins in-volved in the binding of circulating leukocytes tovarious target cellsll, 2]. The interaction(s) be-tween the selectins and the target cells is mediatedby oligosaccharide structures conjugated to specificligand molecules expressed on the taxget cell sur-faces. These ligand molecules may be recognized byone, two or all three of the selectins[1-31. AlthoughaVailable data suggest that the interaction(s) be-tween the selectins and their…     

Proton NMR investigation of heme and surrounding proton in low-spin cyanide-ligated bacterial hemoglobin fromVitreoscilla

1H NMR spectra of low-spin cyanide-ligated bacterial hemoglobin from Vitreoscilla (VtHb-CN) are reported. The assignments of the 1H NMR spectra of VtHb-CN have been made through MCOSY, NOESY, 1D TOE and SUPERWEFT experiments. Almost all resonance peaks of heme and ligated His85 are identified. The spin-lattice relaxation time T1's and the variation relationships of chemical shifts of these peaks with temperature have been acquired, from which the distances between the measured protons and Fe3 , and the diamagnetic chemical shifts have been acquired, respectively. The ionization constants of pKa's of ligated His85 are determined through pH titration of chemical shift, which is 4.95 for ligated His85 C2H proton. The lower pKa is attributed to the influence of the Fe3 of carrying positive charge and the coordination of His85 and Fe3 of heme.     

乙型肝炎表面抗原单克隆抗体的制备及其应用

目的：研制能够同时检测乙型肝炎表面抗原(HBsAg) 野生株和多种变异株的单克隆抗体（mAb）,将筛选出的mAb进行纯度、免疫学性状鉴定,并对其应用效果及质量做初步评价。方法：用中国乙型肝炎病毒感染者血清中分离的HBsAg免疫小鼠,利用杂交瘤细胞融合技术制备抗HBsAg野生株和多种变异株的mAb,将筛选出的mAb经饱和硫酸铵纯化后通过聚丙烯酰胺凝胶电泳、琼脂糖凝胶电泳、ELISA鉴定其纯度、特性,初步评价其应用效果及质量。结果：获得了一株能够同时检测HBsAg野生株和多种变异株的mAb,命名为D12。其纯度较高,特异性强,灵敏度好,Ig亚类测定结果为IgG1,识别位点存在于自然抗原上,其检测HBsAg野生株的能力优于现行3种国产试剂盒,检测HBsAg变异株的能力明显优于现行5种国产试剂盒。结论：成功研制了可以同时识别HBsAg野生株和大多数变异株的mAb,为进一步提高我国当前乙型肝炎病毒变异株的检出率以及加强预防和控制乙型肝炎病毒的传播奠定新的基础。     

《农业与环境生物技术文摘》

《农业与环境生物技术文摘》创刊于 1993年 ,是世界著名的剑桥科学文摘 (ＣＳＡ)近 40个系列检索刊物之一。该刊内容广泛 ,涉及微生物、植物、动物有关的生物技术研究 ,其中农业应用包括动植物病害的诊断与控制 ,生物技术在动植物育种、害虫防治、土壤微生物、废弃物处理、环境污染、能源、矿物和发酵及工艺工程中的应用。该刊把农业与环境生物技术分为 12个一级类目、34个二级类目、4个三级类目 :一般热点与评述专利遗传工程　微生物、植物、动物、克隆载体生物信息学与计算机应用方法　固定、色谱、细胞培养、深低温保藏、新细胞系、免疫…     

蓟马采集和玻片标本的制作

介绍蓟马标本的采集方法、常规鉴定用的临时性玻片和存档及分类用的永久性玻片标本的制作方法。标本的采集主要是拍打植物花朵、叶片及枯枝,玻片的制作重点介绍了制作存档和分类用的永久性玻片的5个步骤,即浸解脱色、洗涤、脱水、整姿封盖和干燥。