受体介导内吞引起的巨噬细胞胞质酸化和胞吐作用

The effects of Con A, WGA, Zymosan A on macrophage cytosolic pH and outflow of lysosomal content through exocytosis were studied with SNAFL-calcein and FITC-Dextran on ACAS570. The results showed all three ligands could induce macrophage cytosolic acidification in about 10 min and kept at the same level hereafter; outflow of lysosomal fluorescent probe through exocytosis appeared in 15-20 min. In resting conditions, macrophage lysosomes mainly distributed in cell center; after stimulated for 15 min by three ligands, the number of lysosomes increased in membrane periphery, in 25-30 min lysosomes moved back toward cell center. We proposed that ligands induced lysosomal pH rises was a basic factor for outflow of lysosomal content through exocytosis, cytosolic acidification inhibited receptor-mediated endocytosis. Cytosolic acidification and outflow of lysosomal content through exocytosis were the results of cellular self-regulation and self-protection during receptor-mediated endocytosis.     

胞质分裂阻滞细胞的微核检测及方法学初步研究

培养人体外周血淋巴细胞的微核检测,是近年来常用的体外短期检测方法之一,可用来评价被检理化因子对人体细胞的遗传毒性,及监测环境、职业接触等有害因子对人类的潜在致癌作用。为了进一步提高该方法的敏感性,根据微核起源于染色体断片与落后染色体的观     

两种药用金龟甲的生物学特性

独角仙AllomyrinadichotomaL．和屎克螂CatharsiusmolossusLinnaeus均属鞘翅目，前者属独角仙科后者属金龟子科。两种昆虫都是重要的虫药，有定惊被瘀、通便攻毒、消癌之功。我们在研究它们的人工饲养技术时发现生物学特性与一般昆虫有一些特异之处，现整理报道如下。！独角他越冬的老熟幼虫即将化蛹2前，逐步从深土层移向浅土层，虫体不断分泌粘液，并长时间不断扭动身体，使周围的泥土、草犀利碎枝形成内壁十分光滑、而外壁非常粗糙的硕大长圆形泥球——蛹室。蛹室长10～12cm，宽7～8cm，壁厚2～3cm（图1）。卵散产于浅土层内．其卵周粘…     

两种雌性溜蝇记述

Based on the examination of Muscidae flies collected from Liaoning and Shanxi Provinces, two females of the genus of Lispe are firstly reported here. Morphological terminology generally follows McAlpine (1981). Absolute measurements are used for body length in millimeters (mm). Abbreviations used for characters include: ad = anterodorsal seta, av = anteroventral seta, p = posterior seta, pv = posteroventral seta. 1. Lispe hebeiensis Ma et Tian, 1993 (Figs. 1~2) Lispe hebeiensis Ma et Tia…     

树莓营养成分分析及其利用

一、前言树莓属蔷薇科(Rosaceae)悬钩子属(Rubus)植物。树莓发展历史悠久,据记载第四世纪就有树莓的种植记载。世界各国有450种,主要分布寒带和温带,少数分布在热带和南半球,我国的树莓共194种。     

微针及其在生物诊疗中的应用研究进展

微针在过去几十年中发展迅速,在透皮给药等领域已得到了广泛的研究.近些年随着微电子技术的蓬勃兴起,微针在生物诊疗中的应用越来越多的受到人们关注.通过微针提取血液和组织液进行检测分析以及微针作为电极在体液内直接对血糖、黑色素瘤、pH值等进行实时检测方面,都展现出了良好的实时检测应用前景.文中综述了微针的材料与结构设计及其在...