云南人群系统性红斑狼疮与TNF微卫星基因座关系的研究

位于MHC内的肿瘤坏死因子（tomornecrosisfactor,TNF）基因区域有5个微卫星基因座a、b、c、d和e,本研究调查了中国云南汉族系统性红斑狼疮（systemiclupuserythematosus,SLE）与这几个微卫星基因座多态性的关系。本文用荧光标记引物和半自动基因扫描方法对云南地区97例SLE患者及79例健康对照者的这5个微卫星基因座进行了基因分型,结果发现,SLE患者的TNFa1（P=0.0206）,c2(P=0.0000)等位基因频率较正常人显著增高,而TNFa2(P=0.0163),c3(P=0.0065),c4(P=0.0012),d6(P=0.0448)等位基因频率则是正常对照较SLE患者高。同时我们还发现在这5个微卫星基因座中,与白种人比较中国人出现了尚未发现的新等位基因。 Abstract：We have investigated TNF microsatellite polymorphism in SLE.A total of 97 Chinese Han SLE patients and 79 matched Chinese Han controls were studied in this study, TNF microsatellites a,b,c,d and e were typed using fluorescent labeled automated genescanning and genotyping.TNFa1（P=0.0206）,c2 (P=0.0000) allele frequencies were significantly increased in the SLE group comparing with controls,and TNF a2 (P=0.0163) c3(P=0.0065),c4(P=0.0012),d6(P=0.0448) allele frequencies were significantly increased in controls.Meanwhile,we found some new alleles in Chinese which are different form those in Caucasoids.     

The algae-lytic ability of bacterium DC10 and the influence of environmental factors on the ability

Nowadays, eutrophication and massive accumula-tions of microalgae have caused serious environmental problems. So it is very important to find an effective method for controlling the growth of the algae and the algal blooms. Measures for controlling the algae in-clude physical, chemical and biological means. Physi-cal means are limited and usually act as an assistant for controlling the algae, and include mechanical means, high frequent electromagnetic pulses and ul-trasonic processes[1]. Algae…     

Multiple Data Sources Reveal a New Asian Leaf Litter Toad of Leptobrachella Smith, 1925 (Anura,Megophryidae) from Southwestern China

A new species of Leptobrachella is described from Sichuan Province and Chongqing Municipality,China.Molecular phylogenetic analyses based on mitochondrial and nuclear gene sequences indicated that the new species is genetically divergent from its congeners.It could be identified from its congeners by a combination of followings characters:body size of male 29.1-34.1 mm(n=14),female 34.1-34.9 mm(n=4);dorsal skin rough with large tubercles in size of humeral glands,without conical spines;fringes o...     

A New Species of the Genus Hebius Thompson, 1913(Serpentes: Natricidae) from Yunnan,China

A new keelback snake of the genus Hebius,H. jingdongensis sp. nov., is reported from Jingdong in Yunnan, China based on a combination of molecular and morphological evidence. Although closest to H.khasiensis and H. boulengeri phylogenetically, this new species is genetically distinct amongst congeners with the mitochondrial Cyt b uncorrected p-distance ranging from 9.5%(vs. H. octolineatus) to 14.8%(vs.H. andreae). In addition, this new species can be distinguished from its congeners by a combin...     

一株高效利用木糖的酵母菌的分离及鉴定

从256个自然试样中筛选到1株高效转化D-木糖为木糖醇的酵母菌株441-28—1。初始木糖质量浓度为90g/L的条件下,24h内的木糖利用效率为3．0g／（L·h）。通过高效液相分析,菌株441-28—1的主要代谢产物为木糖醇。在初始木糖质量浓度为65g/L的条件下,摇瓶分批发酵,木糖醇生成速率达1．1g／（L·h）,木糖醇转化率为70％。经过形态、生理生化特征测定,以及ITS序列分析（GenBank的登记号为EU121523）,将441-28—1菌株鉴定为热带假丝酵母（Candida tropicalis）。Candida tropicalis（热带假丝酵母）已保存于中国高校工业微生物资源数据平台,保藏编号CICIM Y0092。     

云南绵羊线粒体DNA遗传多态性研究

本研究用12种识别六碱基的限制性内切酶对来自云南省3个地方绵羊品种11个样本的线粒体DNA(mtDNA)进行了限制性片段长度多态性分析(RFLP),结果表明,云南绵羊群体平均核苷歧异度为0.086%,平均遗传距离为0.00 4,说明mtDNA变异度很低,遗传多样性贫乏,提示云南绵羊可能起源于同一个共同祖先;昭通绵羊与德钦绵羊距离较远,大约分歧于30万年前,德钦绵羊与腾冲绵羊大约分歧于11万年前,而腾冲绵羊与昭通绵羊也于18万年前发生分歧。 Abstract:mtDNA from 11 sheeps samples of Deqin,Zhaotong and Tengchong in Yunnan province was analyzed with 12 restriction endonucleases.14 restriction types were observed which could sorted into 3 haplotypes.The estimated number of nucleotide substitutions per site(π)in Yunnan sheep was 0.086% and the mean genetic distance(P) was 0.004.This results might show a pattern of single origin of Yunnan sheep.Divergencetime of the native sheep flocks from three districts of Yunnan were estimated on the mean rate of sequence divergence of 0.01 per million years in mtDNA.     

RAPD分析用的犁DNA提取方法

ＲＡＰＤ分析用的梨ＤＮＡ提取方法胡春根郝玉邓秀新史永忠（华中农业大学作物遗传改良国家重点实验室,武汉４３００７０）ＤＮＡＥｘｔｒａｃｔｉｏｎＭｅｔｈｏｄｆｏｒＲＡＰＤＡｎａｌｙｓｉｓｉｎＰｅａｒｓＨＵＣｈｕｎｇｅｎＨＡＯＹｕＤＥＮＧＸｉｕｘｉｎＳＨＩ...     

Effect of Morphology and Age on the Closure Ability of Asian Box Turtles(Cuora)

The ability of box turtles to close their shell as an antipredation adaptation and the potential impact of the anterior and posterior lobes of the plastron on the closing force and closing time remain relatively unexplored. Here, keeled(Cuora mouhotii) and flowerback(C. galbinifrons) box turtles, whose shell cannot and can be completely closed, respectively, were studied. Anterior and posterior closing forces were measured using a force transducer, and the closing time was recorded. The anterior...     

Critical Role of Cys168 in Noggin Protein's Biological Function

Previous studies have indicated that noggin exerts its neural inducing effect by binding andantagonizing bone morphogenetic protein 4(BMP4).In order to further clarify the relationship between thestructure and the function of noggin,and elucidate the possible mechanism responsible for noggin-BMP4interaction,we generated three noggin mutants,C168S,C174S and C197S,by using a site-directed mu-tagenesis method.Ectopic expression of wild-type(WT)noggin,C174S or C197S,in Xenopus animal caps(ACs)by mRNA injection converted the explants(prospective ectoderm)into neural tissue,as indicated bythe neural-like morphology and expression of the neural cell adhesion molecule(NCAM)in the ACs.Incontrast,ACs expressing C 168S suffered an epidermal fate similar to the control caps.Similarly,among the threemutants,only C 168S lost the dorsalizing function.These studies highlight the critical role played by Cys168in noggin's biological activities.It probably participates in the formation of an intermolecular disulfide bridge.     

DXS6804/DXS9896/GATA144D04基因座在中国汉族群体中的遗传多态性及其法医学应用Genetic

为了调查X染色体上DXS6804、DXS9896和 GATA144D04等3个STR基因座在中国汉族群体的遗传多态性及其法医学应用价值,来用PCR和聚丙烯酰胺凝胶电泳对X染色体3个STR基因座进行分型,并检验女性基因型频率分布是否符合Hardy-Weinberg平衡,计算法医学常用各种概率。DXS6804、DXS9896和 GATA144D04的非父排除率分别为0.5990、0.6220、0.4280,表明3个STR基因座在中国汉族群体均具有遗传多态性,χ2检验表明女性的基因型频率分布符合Hardy-Weinberg平衡。X染色体上的基因座DXS6804、DXS9896和 GATA144D04在中国汉族群体中具有较高的遗传多态性,可应用于法医学检验和群体遗传学分析。 Abstract： To investigate the genetic polymorphisms of three short tandem repeats loci of chromosome X in Chinese Han population in Chengdu area and its use in forensic science. Three X-chromosome linked short tandom repeat loci were analyzed by PCR followed by polyacrylamide gel electrophoresis. Hardy-Weinberg equilibrium was tested and forensic interested value was calculated .The power of exlcution of DXS6804、DXS9896和 GATA144D04 is 0.5990、0.6220、0.4280,respectively. The result showed that all the three STR loci were polymorphic among 100 unrelated females and 120 unrelated males from Chinese Han population. χ2 tests demonstrated that genotype frequencies in females did not depart from Hardy-Weinberg equilibrium. Three X-chromosome linked short tandem repeat loci have high polymorphism, they can be applied to forensic medicine and population genetics.