Polystyrene substratum for bulk culture of anchorage dependent cells

Twisted ribbons made of polystyrene were used as a packing material for the cultivation of anchorage dependent cells. Normal human fibroblast cells grown on this support in a laboratory scale reactor reached densities of about 5–7×10⁵ cells/ml. The cells adhered strongly to the carrier and no cell detachment was observed upon transfer to serum free medium. The properties of this packing material and its potential use are discussed.     

Fixed bed porous glass sphere (porosphere) bioreactors for animal cells

Process intensity of fixed bed glass sphere culture systems is increased considerably by replacing solid glass spheres with open pore glass spheres. This technique demonstrates the possibility of having a system capable of both volumetric and cell density scale up and being suitable for substrate attached and suspension cells. The yields achieved for a number of attached cell lines (approximately 10⁷/ml) demonstrate an increase approaching one order of magnitude over solid glass spheres (approximately 10⁶/ml). Also suspension cells were successfully entrapped in the open pore structure with similar yields.     

Purification and characterization of lymphocytic clonal growth factor (LCGF) derived from human-human hybridoma SH-76 cells

Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW column chromatograph. The purified factor was a 72K single protein. The factor showed marked growth stimulating effect on lymphocytic cell lines, but had no effect on the growth of human adhesive cancer cell lines. Thus, the factor is a lymphocytic clonal growth factor (LCGF), as found previously in human plasma (Miyata, 1988). The LCGF of SH-76 cells could be produced in growth factor-free RPMI medium and purified easily from the conditioned medium. The factor is inactivated by heating at over 80°C, but is much more stable than the LCGF in human plasma.     

一阶导数光谱法测定猫豆中左旋多巴的含量

川一阶导数光谱法测定猫豆中主旋多巴的含量,可以排除无关杂质吸收的干扰,省去复杂的分离提取步骤。试验结果表明:相关系数达0.9999,平均回收率101.5％,重现性较好。     

若干铁杉属植物核型的比较研究

本文首次报道了我国特产的重点保护植物南方铁杉的核型,全由中部和近中部着丝点染色体构成,核型公式为K(2n)=2x=24=20m+4sm,属“ 2A”类型。染色体的相对长度组成为2n=24=12M_2+10M_1+2S。通过比较。发现东亚的南方铁杉的核型与台湾铁杉甚为相似而略具进化的趋势,但北美东部的卡罗来纳铁杉的核型比它们进化得多。本文支持Florin认为铁杉属至少在早第三纪存在一条从欧亚大陆经过白令海峡到达北美洲的迁移路线的意见。     

植物新药——甘木通的生药鉴别

木文对甘水通的生药结构、药用情况、鉴别点、地理分布及繁殖等进行了报道;同时指出造成混乱的原因及找出混乱种的学名。甘本通为分布于广东和广西两省区的特有种。     

渤海海域下第三系渐新统东营组下段的三个介形类新属

本文记述了非海相介形类的三个新属——Tianjinia gen.nov., Shaozengia gen.nov.和Bohaicythere gen.nov.,并依据Tianjinia的闭壳肌痕特征建立了一个新亚科——Tianjininae subfam。nov.。这三个新属的材料均采自渤海海域下第三系渐新统东营组下段。它们的发现,为进一步研究渤海盆地早第三纪介形类动物群的演化、生态环境提供了新的材料。文中对三个新属的生存环境作了探讨。     

山西垣曲和山东蒙阴等地始新世介形类

山西垣曲县垣曲组、山东蒙阴县官庄组和临朐县五图组中的介形类化石,计15属31种,其中1新属18新种及5未定种。这些介形类化石对我国第三纪地层,特别是对第三纪含油地层的划分对比具有重要意义。     

微体化石的处理

本文较全面地介绍一些微体化石的实验室处理方法,它包括岩样的破碎、松样、冲洗和挑选等各方面的技术问题,以及在分析处理过程中应注意的事项。     

雄性华蓥库蠓的描述及雌蠓特征的补充(双翅目:蠓科)

华蓥库蠓Culieoides huaylngensis Zhou et Lee,1984 动物分类学报9(3): 293—294 根据四川省华蓥山一只雌蠓标本记述,雄蠓未详。冷延家教授于1983年5月15日在江西大余的吉村采获一批库蠓标本,经作者鉴定发现华蓥库蠓1♂5♀♀,从而获得本种雄蠓。现记述于后,并对雌蠓特征作了补充。标本保存于沈阳市卫生防疫站。