人表皮生长因子基因的化学合成和克隆及其在酵母中的表达

用固相亚磷酰胺法合成了人表皮生长因子基因,全长为173个核苷酸,它被分成8个寡核苷酸,分别在DNA合成仪上合成。经分离纯化后的寡聚核苷酸进行酶促连接,然后被克隆到噬菌体M13mpl8中,克隆经分子杂交、限制酶酶切以及DNA序列分析检测,证明合成的人表皮生长因子基因和设计的完全一致。将人表皮生长因子基因的DNA片段插入酵母分泌型表达载休YFD59 HindⅢ位点中,建成表达人表皮生长因子基因的质粒YFDl04,再将此质粒转化酿酒酵母,所得转化子经摇瓶发酵,发酵上清液用受体结合试验表明有人表皮生长因子表达。     

黄土丘陵半干旱区不同作物生态效能研究

深入研究特定生态环境条件下不同作物生产力及其生态效能,对于合理调控农田生态系统,探讨提高系统生产力途径具有重要意义。60年代以来,在国际生物学计划(IBP)的影响下,美国、日本、菲葎宾等国先后进行了小麦、水稻、大豆等作物农田生态效能研究。我国于“六·五”期间分别在南方稻区、黄淮海平原以及北方灌区等不同农业区开展了多种作物种植制度生产力问题研究,并取得重     

甲肝病毒在人血白细胞中体外连续传代和在分类白细胞中增殖的研究

1985年我们采用间接免疫荧光法(IF法)检测出甲肝患者外周血白细胞中有甲肝抗原(HAAg)存在,继而又将HAAg阳性白细胞直接种入PLC/PRF/5细胞,分离到两株甲肝病毒(HAV)NJ—3株和H—1株。为了弄清白细胞所携带的病毒究竟仅为吸附吞饮,抑或能在其中复制增殖,我们将分离到的HAV用正常人血白细胞进行体外增殖试验,现将结果报告如下。     

漫谈皮纹学

狡猾的犯罪老手,一定会戴上手套作案。这些惯犯心里明白,在作案现场留下指纹,无疑会给公安人员破案提供线索和佐证。我国西安出土文物秦简的“盗穴篇”就显示了我们的祖先早在2000多年前就懂得了“手迹”的含义。利用指纹破案,由来已久。人的手指、手掌、足趾、足蹠等皮肤上出现的纹理图型,就称皮纹。皮纹是人类重要的遗传特征,具有家族遗传性、高度稳定性及个体特异性三大特征。皮纹的结构是由遗传决定的。它在胚胎发育过程中,一经形成,终生不变。每个人的皮纹,绝不相同。英国科学家高尔顿(Gaiton)在1892年就曾推测,当地球上增至60亿人口时,也许才能找到指纹特征完全相同的两个人。因此,皮纹可作为绝妙的天然“身份证”。科技先进国家的     

冬眠周期长短不同的蒙古黄鼠（Citellus dauricus）...

Adult Mongolian ground squirrels (Citellus dauricus) were kept at 5 degrees C in winter and divided into four experimental groups according to the bout length. The first group was not hibernating until decapitation. The bout length of the second group was between 4-10 days, the third group 11-17 days and the fourth group longer than 20 days. All pineals were sampled at the end of January. Morphometric analytical procedures were used to study the ultrastructure of the distal part of the pineal gland. The statistical results demonstrated that 1) the euthermic animals have larger cross areas of pinealocyte, longer and narrower Golgi apparatus and more number of saccules of each Golgi apparatus (P less than 0.01). But they also have smaller volume density of vaculoes, less lipid droplets and associated vesicles around Golgi apparatus (P less than 0.01). 2) the hibernating animals with variety of bout length had no significant differences in the number of mitochondria, lipid droplets, lysosomes, the size of Golgi apparatus and the cross areas of nucleus and cytoplasm (P greater than 0.05). However, the number and the cross areas of vacuoles were significantly increased with the bout length (P less than 0.01). This might suggest that the bout length was not related to the metabolic activity of pinealocytes in Citellus dauricus and vacuoles might play some important roles in maintenance of individual bout of hibernation in this species.     

间日疟原虫卵囊内成孢子细胞与子孢子形成的超微结构观察

应用透射电镜观察间日疟原虫在大劣按蚊体内发育的卵囊内成孢子细胞及子孢子形成过程形态变化。疟原虫采自带有配子体的间日疟自愿者。蚊虫在感染后8天作解剖。本研究观察结果与前人所描述的柏氏疟原虫和鸣疟原虫的成孢子细胞与子孢子形成过程相似,即成孢子细胞形成开始于卵囊被膜下的周围出现液泡,而随着膜下液泡增大,逐渐向胞质延伸并联接成裂缝,使胞质再分裂而形成。子孢子周围的膜下微管分布不对称,其数目和排列型式,多数为:7+4、7+5、8+4和8+5,少数为10+1,与前人报告不同(10+1)。     

鲍氏志架氏菌的一个新血清型

Two strains which belong to the same serotype of Shigella were isolated from the bloody-pus stool of two patients (in 1986) and is reported in this paper. The results were identical both showing agglutination in low titer with serotype 8 of S. dysenteriae and serotype 4 of S. boydii when the two strains were checked well with all kinds of diagnostic antisera and vice versa, ie the antisera produced by the two strains were also checked well with sera prepared with the representative strains of all Shigella spp. No cross agglutination with O6, O7, and O150 of E. coli were found. Consequently, It appears to be a new serotype of Shigella. These two strains possess the ability of causing keratitis in guinea-pigs as well as invading epithelial cells, the DNA of both strains in agarose-electrophoresis showed a large plasmid, indicating that they are virulent strains possessing invasive ability. It was concluded that these two strains belonged to Shigella boydii as they fermented mannitol and non-related antigenically with Shigella flexneri. Since serotype 1-18 of S. boydii have been reported recently, we propose that this new serotype should be serotype 19 of Shigella boydii.     

(山王丌)岗通城虎化学成分的研究

<正> (山王丌)岗通城虎Aristolochia longgangensis C.F.Liang.民间用于治疗小儿高烧,损伤,虫伤等疾病。(山王丌)岗通城虎是一新种,其化学成分还未研究过。我们对其化学成分进行了研究,报告如下: 采用热乙醇提取其总成分,然后用冷乙醇溶解,再用石油醚洗涤,不溶物用硅胶柱层析