排序方式: 共有74条查询结果,搜索用时 15 毫秒
21.
黄蜀葵的化学成分研究 总被引:15,自引:0,他引:15
黄蜀葵花对多种口腔炎症有止痛作用,治疗慢性气管炎疗效亦较好。从其酒精浸膏中分离出五种黄酮类成分,经光谱分析、衍生物的制备、酸水解以及物理化学常数的测定,分别鉴定为槲皮素-3-洋槐糖甙、槲皮素-3′葡萄糖甙、金丝桃甙、槲皮素及杨梅素。其中槲皮素-3-洋槐糖甙及槲皮素-3′-葡萄糖甙在本属植物中系首次分得。 相似文献
22.
Karyotyping of Brassica napus L. Based on C0 t-1 DNA Banding by Fluorescence In Situ Hybridization 总被引:7,自引:0,他引:7
Wen-Hui WEI Wan-Peng ZHAO Li-Jun WANG Bo CHEN Yun-Chang LI Yun-Chun SONG 《植物学报(英文版)》2005,47(12):1479-1484
In order to precisely recognize and karyotype Brassica napus L. chromosomes, C0t-1 DNA was extracted from its genomic DNA, labeled with biotin-1 1-dUTP and in situ hybridized. The hybridized locations were detected by Cy3-conjugated streptavidin. Specific fluorescence in situ hybridization (FISH) signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both Cot-1 DNA FISH banding patterns and chromosome morphology. 相似文献
23.
AI Hai-xin ZHANG Li ZHANG Xin-gang CHEN Si-yao HU Huan WEI Hong-yun MENG Xin-rui WANG Tian-qi ZHAO Jian LIU Hong-sheng 《微生物学杂志》2017,(1):20-27
Nattokinase is a fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. In this study, nattokinase was purified from fermentation broth of a Bacillus subtilis strain by ammonium sulfate salting-out, gel filtration chromatography, and hydrophobic interaction chromatography with a purification fold of 5.2 and at a yield of 46.3%. The purified enzyme has molecular mass of 28 kDa and fibrinolytic activity of 4 580 U/mg. Since the concentration of nattokinase on fermentation broth was quite low, we cloned nattokinase gene from B. subtilis and expressed it in E. coli BL21 (DE3). Nattokinase was actively expressed in the recombinant strain. The yield of nattokinase was increased significantly, but the activity of the protein produced by recombinant strain was low. 相似文献
24.
XI CHEN WEI ZHANG YUN FEI GAO XIAO QIN SU ZHONG HE ZHAI * College of Life Sciences Peking University Beijing China The Key Laboratory of Cell Proliferation Regulation Biology of the Ministry of Education College of Life Sciences 《Cell research》2002,(Z1)
P21Waf1/Cip1 is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21Waf1/Cip1 involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidence for a link between p21Waf1/Cip1 and cellular senescence. While in murine cells, the role of p21Waf1/Cip1 is indefinite. We explored this issue using NIH3T3 cells with inducible p21Waf1/Cip1 expression. Induction of p21Waf1/Cip1 triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features, such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed that p21Waf1/Cip1-transduced NIH3T3 cells expressed β-galactosidase activity at pH 6.0, which is known to be a marker of senescence. Our results suggest that p21Waf1/Cip1 can also induce senescence-like changes in murine cells. 相似文献
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26.
以 pPICZαB为载体 ,应用RT PCR从感染D2V的C6 / 36病变细胞中克隆全长E基因 ,电转化法将重组质粒整合入巴斯德毕赤氏酵母菌 ,经抗生素筛选、表型鉴定和PCR分析得到Mut 型的多拷贝整合菌 ,经甲醇诱导培养可产生 6 9kD的融合蛋白 ,与含组氨酸尾的D2V包膜糖蛋白分子量理论值相符 ;免疫印迹证实该表达产物可与D2VE特异性单抗和D2V多抗进行反应 ;表达产物经金属螯合亲和层析可获得纯化的含组氨酸尾的E融合蛋白并保留其免疫反应性。研究显示克隆的全长D2VE基因可在毕赤氏酵母菌中高效分泌表达 ,E融合蛋白最大表达量 0 .1g/L。 相似文献
27.
Immunotherapy of tumor by targeting angiogenesis 总被引:4,自引:0,他引:4
HOU Jianmei TIAN Ling & WEI Yuquan Key Laboratory of Biotherapy of Human Diseases of Ministry of Education West China Hospital of Sichuan university Chengdu China Correspondence should be addressed to Wei Yuquan 《中国科学:生命科学英文版》2004,47(6):545-552
As early as 1971, Folkman proposed that thegrowth of and persistence of solid tumors and theirmetastasis depended on an adequate blood supply and,therefore, an anti-angiogenic strategy might be effec-tive as an anticancer therapy. As a strategy for cancertherapy, anti-angiogenic therapy attempts to stop newvessels from forming around a tumor and break up theexisting network of abnormal capillaries that feed thecancerous mass[1,2]. Anti-angiogenic therapy take someadvantages over the convention… 相似文献
28.
魏丽珠 伏洁 刘光陵 王晓燕 王兆全WEI Li-zhu FU Jie LIU Guang-ling WANG Xiao-yan WANG Zhao-quan 《遗传》1999,21(2):13-15
为了探讨神经肌肉性疾病的发病与线粒体DNA突变的关系,采用PCR技术检测了 20例患有不同神经肌肉性疾病儿童的外周血和骨骼肌细胞中的线粒体DNA(mtDNA),发现其中6例患儿有mtDNA缺失,其中1例至少有2968bp片段的缺失, 另5例至少有2000bp片段的缺失,此缺失区位于线粒体呼吸链复合物1、 4、5、编码区,表明该突变对神经肌肉性疾病的发生有一定作用。
Abstract:To understand the relation to mechanism of neuromuscular disease and mtDNA mutation,using PCR technique,we investigated blood and /or skeletal muscle of 20 patients with neuromuscular diseases.A deletion in the length of 2000~2968bp was found in blood mitochondrial DNA of 6 patients with neuromuscular disease.The deletion region partially lies in the coding region of resoiratony chain complex 1,4,5.It is suggested that this mutation ois related with neuromuscular diseases. 相似文献
29.
WEI XINHUA YONG ZHAO XIAOMING DONG YAXIAN SU ZILI MA CHANGXIN ZHU SHIJINPANGDept. of Cell Biology Beijing Medical University Beijing China.Beijing Vacuum Physics Lboratory Academia Sinica Beijing China. 《Cell research》1993,(1)
The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smaller and bigger peaks was obtained by scanning tunneling microscope(STM), looking like the domains of lipid groups and proteins in the model of fluid mosaic biomembrane. Some even more higher and wider peaks projected out from the membrane surface in STM image after the interacting of laminin with membrane receptor were, probably, the complexes of laminin and membrane receptor. Furthermore, the decreased lateral diffusion coefficient value (D) was obtained by fluorescence recovery after photobleaching (FRAP) after the laminin was reacted with membrane receptor. This phenomenon provides an evidence that the complexes of laminin and its membrane receptor were located on the membrane of macrophages. So we could consider that the laminin is combined with membrane receptor leading to the variation in the properties of membrane surface. 相似文献
30.
细锥香茶菜甲素和乙素的化学结构 总被引:1,自引:0,他引:1
从细锥香茶菜中分得两个新的二萜类化合物,命名为细锥香茶菜甲素和乙素(coetsinA&B)根据其光谱和化学数据,推定其化学结构为[1]和[2]。 相似文献