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To investigate the inhibitory effect of the Bcl-XL small interfering RNA(siRNA)on BcI-XLgene expression in the human gastric cancer cell line MGC-803,green fluorescent protein(GFP)siRNAwas constructed and transfected into MGC-803 ceils,together with GFP expression vector pTrace SV40.GFP expression levels were observed using fluorescence microscopy.Bcl-XL siRNA and negative siRNAwere then constructed and stably transfected into MGC-803 cells.RT-PCR and immunofluorescence wereused to detect the expression of Bcl-XL.Spontaneous apoptosis was detected by acridine orange(AO)andflow cytometry.Results were as follows:(1)48 h after GFP expression vector and GFP siRNA co-transfection,the expression level of GFP in the GFP siRNA group was much lower than the negative siRNA group,according to fluorescence microscopy results.The mRNA and protein levels of Bcl-XL in Bcl-XL siRNAstable transfectants were reduced to almost background level compared with negative siRNA transfectantsor untreated cells.(2)Changes in nucleus morphology was observed by AO staining nucleic and flowcytometry analysis,which showed that stable Bcl-XL siRNA transfectants have an increased spontaneousapoptosis (21.17%+1.26% vs.1.19%+0.18% and 1.56%+0.15% respectively,P<0.05 vs.negative siRNAor untreated control),siRNA targeting GFP or Bcl-XL genes can specifically suppress GFP or BcI-XLexpression in MGC-803 cells,and Bcl-XL siRNA can increase spontaneous apoptosis.Bcl-XL siRNA maybe a beneficial agent against human gastric adenocarcinoma. 相似文献
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Nuclear translocation of EGF receptor regulated by Epstein-Barr virus encoded latent membrane protein 1 总被引:3,自引:0,他引:3
TAO Yongguang SONG Xin TAN Yunnian LIN Xiaofeng ZHAO Yan ZENG Liang TANG Min LI Wei WU Qiao & CAO Ya . Cancer Research Institute Xiangya School of Medicine Central South University Changsha China . Key Laboratory of the Ministry of Education for Cell Biology Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen China 《中国科学:生命科学英文版》2004,47(3):258-267
Epstein-Barrvirus(EBV),oneoftheDNAon-cogenicviruses,iscloselyassociatedwiththegenesisofBurkitt抯lymphoma,undifferentiatednasopharyn-gealcarcinoma(NPC),Hodgkin抯disease,gastriccancerand,lungcancer,etc.[1].EBVencodedlatentmembraneprotein1(LMP1)isconsideredtobethemajoroncogenicproteinofEBVencodedproteins.Biologicallyspeaking,LMP1isanintegralmembraneproteincontaining386aminoacids.Thethreedo-mains(CTAR1,CTAR2,CTAR3)intheC-terminusofLMP1havebeenshowntoinitiatethesignalingproc-ess.The… 相似文献
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张野 忻仁娥 江三多 汤国梅 钱伊萍 李飞 汪栋祥ZHANG Ye XIN Ren-er JIANG San-duo TANG Guo-mei QIN Yi-ping LI Fei WANG Dong-xing 《遗传》2001,23(5):413-750
为探讨注意缺损多动障碍(ADHD)与多巴胺D4受体基因(dopamineD4receptorgene,DRD4)间的关系,采用Amp-FLP的方法检测了上海地区汉族人群中68例ADHD患者及其父母DRD4的多态性,数据采用基于单体型的单体型相对风险(HHRR)及传递不平衡检验(TDT)进行遗传关联分析.结果表明HHRR分析和复等位基因的TDT检验,均未显示出与ADHD的遗传关联性(P>0.05).提示上海地区人群中DRD4基因与ADHD无显著性关联。
Abstract:The present study was designed to investigate the association between attention-deficit hyperactivity disorder (ADHD) and the dopamine D4 receptor gene (DRD4).Amp-FLP was used to test the polymorphisms of DRD4 in 68 ADHD children and their parents from Shanghai.The haplotype-based haplotype relative risk (HHRR) and transmission disequilibrium test (TDT) methods were used to analyze the association between DRD4 and ADHD.In our samples we found no significant association between DRD4 and ADHD (P>0.05).Our results do not support that ADHD is associated with DRD4 in population in Shanghai. 相似文献
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利用视黄酸(RA)诱导小鼠胚胎干细胞通过悬浮类胚培养法体外定量分化为神经样细胞.结果显示能够进行种系嵌合的MESPU22细胞系与不能进行种系嵌合的MESPU13细胞系的体外神经分化比例在统计学上没有明显差异.此结果对于人的胚胎干细胞全能性的鉴定与检测有重要启示。
Abstract: Murine embryonic stem cells were induced to differentiate into neuron-like cells by all-trans Retinotic Acids through embryoid body culture. The results indicate that there is no obvious difference between MESPU22 cell line which is highly germline competent and MESPU13 cell line which has no germline competence. The result has important illumination on the identity of the totipotence of human embryonic stem cells. 相似文献
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袁晓东 李晶泉 王红伟 姜明 汤敏谦YUAN Xiao-dong LI Jing-quan WANG Hong-wei JIANG Ming TANG Min-qian 《遗传》1999,21(6):47-68
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Synthetic pyrethroids are considered as possible sub-stitutesfor some organophosphate carbamates or organochlo-rine insecticides,and have been used extensivelyfor morethantwo decades[1].Pyrethroids are preferred over otherinsecticides because of their easy degradation into non-toxic or less toxic metabolites under natural conditions.Consequently,there has recently beena dramatic increaseinthe use of pyrethroid pesticides to control insect pests.However,synthetic pyrethroids were also reported … 相似文献
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With the development of modern agriculture,envi-ronmental contamination by a variety of organic toxicagents has been detected in many freshwater systems.These include herbicides,insecticides,surfactants andother organic compounds,which have,been reported byStratton[1]and many other authors to affect boththeir tar-get and non-target organisms whenever dischargedintotheenvironment,thus impairing heavily on the structure andfunction of ecosystems.Therefore,it is necessary to de-velop pesticides w… 相似文献
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LI JinE GUO ZhengYan HUANG Wei MENG XiangXi AI GuoMin TANG GongLi CHEN YiHua 《中国科学:生命科学英文版》2013,56(7):619-627
Streptothricins (STs) are used commercially to treat bacterial and fungal diseases in agriculture. Mining of the sequenced microbial genomes uncovered two cryptic ST clusters from Streptomyces sp. C and Streptomyces sp. TP-A0356. The ST cluster from S. sp. TP-A0356 was verified by successful heterologous expression in Streptomyces coelicolor M145. Two new ST analogs were produced together with streptothricin F and streptothricin D in the heterologous host. The ST cluster was further confirmed by inactivation of gene stnO, which was proposed encoding an aminomutase supplying -lysines for the poly-β-Lys chain formation. A putative biosynthetic pathway for STs is proposed based on bioinformatics analyses of the ST genes and experimental evidence. 相似文献
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