芫荽酵素液的制备及其去腥功效研究

以芫荽茎叶为主要原料,利用复合生物菌种发酵制备芫荽酵素液,对酵素液外观、糖度、酸度以及多酚、黄酮等含量进行了测定,并对其去除水产品中腥味的能力进行了检测.结果显示,芫荽酵素液与未经发酵的原液相比,其活性成分多酚含量提高了33.8％,黄酮提高了41.3％;除腥试验结果表明,芫荽酵素液除腥的最优工艺条件为:酵素液添加量8％...     

高等植物嫁接过程的组织学和细胞学研究

嫁接是我国最早发明的一项农业技术,广泛地应用于农、林、果树生产上。在悠久的历史过程中,积累了丰富的经验。但是对嫁接的基本理论,特别是细胞学方面的研究,基本上     

原发肾上腺淋巴瘤1例并文献复习

目的:探讨原发肾上腺淋巴瘤(PAL)的临床特点,提高对该病的认识.方法:对1例原发肾上腺淋巴瘤患者的资料进行分析,并结合国内外相关文献进行总结.结果:原发肾上腺淋巴瘤好发于老年男性,可表现为局部症状如腹痛,腰痛,也可表现为乏力,发热,体重减低等全身症状,50%病人可出现肾上腺功能不全的症状.一些病人是在行影像学检查时因偶然发现肾上腺的肿物,经病理检查而确诊的.该病多累及双侧肾上腺,最常见的病理类型是弥漫大B细胞型.原发肾上腺淋巴瘤的治疗包括手术、化疗、放疗以及它们的不同组合.该病恶性程度高,进展迅速,预后差.结论:原发肾上腺淋巴瘤虽然少见,但在肾上腺肿瘤,尤其是快速增长的肾上腺肿瘤的鉴别诊断中应考虑到它的可能,及时进行病理检查可尽早确诊.     

双退火温度PCR扩增DNA

【目的】与设置单一退火温度的常规PCR(S-T_m PCR)不同,本研究探讨双退火温度PCR(D-T_m PCR)由高到低设置2条引物各自退火温度。【方法】以PxF61和VPel为正/反向引物,用Q5 DNA聚合酶扩增4.3 kb的模式DNA pET20b-Xyn(黑曲霉木聚糖酶基因)。PCR程序为:98°C预变性3 min,30次循环{98°C变性30 s,设置双退火[T_(m1) 70°C(Px F61)退火15 s、T_(m2) 62°C(VPel)退火15 s],72°C延伸130 s}。【结果】与S-T_m PCR(61°C)相比,D-T_m PCR扩增4.3 kb的目的条带亮度更高,减少2条杂带;经25次循环目的 DNA产物量最高。D-T_m PCR用于长片段引物扩增5.3 kb重组质粒DNA条带更明显。【结论】D-T_m PCR直接扩增目的条带,避免了探讨T_m的麻烦,不要求2条引物T_m相近,从理论上更加清晰地认识引物与各自模板分步退火过程。     

一头食蟹海豹的病理解剖及死因分析

On October 20, 2014, a dead crabeater seal (Lobodon carcinophaga) was found at Fildes Pennisula, King George Island, Antarctica. In order to investigate the causes of the death, external morphological measurements and pathological anatomy were carried out one day later. The specimen was an adult female and looked unusually thin, with body length of 231 cm, body weight of 130 kg, and the subcutaneous fat thickness was only 1.8 cm, significantly lower than that in a healthy seal. No obvious traumas were found on its body, with the exception of a small wound located at the lower right side of body and part of the right eye was missed, which might have been eaten by a Southern giant petrel (Macronectes giganteus) according to the field observations. No pathologic change was found in the internal organs and there was no food residue in the stomach and intestines after the autopsy. However, there was severe problem with the teeth; the incisors and canines had some wear or breaks, and three postcanines were missed and the rest of the postcanines were loose, with the roots becoming black and damaged. In addition, the gum tissue was black and festering. Therefore, it was speculated that the death might be due to starvation caused by the dental problems.     

缅北科考一线故事

正2017年4～5月,在中国科学院国际合作局及东南亚研究中心的支持下,本刊编辑先义杰与特约摄影师蔡石参加了第五次中缅联合科学考察,获得了大量一手资料及影像素材,会陆续在本专辑及后续出版物中呈现给读者。     

不同发酵方式和保存方法对3种生防菌活菌量的影响

为明确3株优良生防菌SC11、153、FO47在不同发酵方法和保存条件下活菌量变化规律,采用活菌平板计数法,测定3种生防菌12个月内活菌量变化及3种生防菌粉剂发酵初期和12月保存期后对茄子黄萎病的温室盆栽防效。结果表明,SC11、 153和FO47菌粉于4 ℃密封保存12个月后活菌量与其初始活菌量差异不显著（P<0.05）;FO47菌粉在4 ℃保存12个月后活菌量高于初始活菌量（P<0.05）;3种生防菌发酵液初始活菌量较菌粉低1～2个数量级,在保存期60 d内活菌量急剧下降,第6个月时接近于0。3种生防菌粉存放12个月后对茄子黄萎病仍保持较高的抑菌活性。说明,固体发酵菌粉可以得到较高数量级的初始活菌量,3种生防菌菌粉4 ℃密封保存12个月后活菌量及抑菌活性不变,且FO47菌粉活菌量略有上升;发酵液不利于生防菌的长期保存。     

超过320个新种的问世 中缅合作新发现

正森林在缅甸生物多样性保护及生态系统服务增强等方面发挥着关键的作用。在国家持续不断的决策过程中,将森林及生物多样性置于核心地位非常重要,它事关区域绿色增长、脱贫、土地可持续利用、减轻及适应因气候变化带来的影响、确保食品安全及营养,以及实现联合国《可持续发展目标》。因此,我们必须