条纹斑竹鲨基因组的RAPD分析初报

采用11种随机引物对4 条条纹斑竹鲨基因组进行了RAPD检测。结果表明, 11种引物在每条个体上扩增的 DNA片段总数在77～84之间, 单个随机引物扩增的DNA片段数目由1至11条不等,平均为7.5条 DNA 片段, 片段的大小在 300～2 800bp之间。个体之间的相似率在90％以上。 Abstracts　4 individuals of Chiloscyllium plagiosum were analyzed by RAPD method using 11 arbitrary primers. For the 11 arbitrary primers, each individual showed 77～84 bands corresponding to amplified products. Each primer gave 1～11 bands for each individual. On average, about 7.5 bands were obtained per primer per individual. The length of the fragment is 300～2 800 bp. The similarity between band profiles of the four individuals was over 90％.     

蓖麻蚕DNA导入家蚕引起遗传变异的研究-基因组DNA的RAPD检测

借助于精子介导,在家蚕受精的过程中将蓖麻蚕DNA转入家蚕卵内,从它们后代获得了新的变异品系。本文采用RAPD技术对这些品系基因组DNA进行了分析。结果表明,所用50种10mer随机引物中有49个检测出DNA的多态性,统计分析图谱中各类扩增带,其中变异品系与其相应受体的差异带占其总带数的26-37%,提示外源DNA导入受体后引起后代基因组的显著变异,并对这些变异的意义了讨论。 Abstract:With the aid of domesticated silkworm sperms,eri silkworm DNA was transferred into domesticated silkworm eggs during insemination,and variant strains were obtained from the progenies.Genomes of three new strains were analyzed using RAPD assay.Polymorphic fingerprints were obtained from 49 out of 50 primers.Different kinds of amplified bands in RAPD patterns were calculated and analyzed,the variant bands between variants and their recipients counted for 26~37% of the total bands of each variant.The results indicated that exogenous DNA introduced into recipients induced remarkable variation in progeny genomes.The significance of the variation was discussed.