噬菌体随机肽库筛选抗志贺样毒素Ⅱ结合亚单位Stx2B的单抗的识别表位

目的利用噬菌体随机肽库技术筛选志贺样毒素Ⅱ结合亚单位Stx2B的单抗的识别表位。方法以抗志贺样毒素Ⅱ结合亚单位Stx2B的单克隆抗体筛选噬菌体随机12肽库,挑取阳性克隆测定DNA序列,推导其氨基酸序列并进行同源性分析。通过ELISA鉴定获得的噬菌体短肽与单抗之间的结合特性。结果从噬菌体随机12肽库中筛选出20株可与抗志贺样毒素Ⅱ结合亚单位Stx2B的单抗特异结合的噬菌体克隆,其中多数克隆呈现核心序列WTSRW（Q）,该序列与志贺样毒素Ⅱ结合亚单位Stx2B的一级序列具有一定的同源性。结论WTSRW（Q）序列是志贺样毒素Ⅱ结合亚单位Stx2B单抗的识别表位。     

赤链蛇染色体组型、C带和Ag-NORs的研究

以骨髓细胞为材料研究赤链蛇的染色体,结果表明该物种2n=46,由8对大型的和15对微小的染色体组成,AF=50.性染色体的大小介于3号和4号之间,为ZW型;8对大型染色体均显示着丝粒C带,1-6号还显示浅染端粒C带.W染色体为整条C带阳性;该物种一对NOR位于7号染色体近着丝粒区.不同地理分布群赤链蛇核型可能经历过Z与W染色体不等交换。 Abstract:The Karyotype,C-bands and Ag-NORs of Dinodon rufozonaturn (Cantor) reported is in this paper including the diploid number(2n=46)comprising 8 pairs of macro-and 15 pairs of microchromosomes,and AF=50 in the D.rufozonatum.The sex chromosome in size locates between the autochromosones No.3 and No.4,which belongs to ZW type.The C-banding technique revealed that the all macrochromosomes there are centromerc C band,the telomeric C band was only observed in Nos.1~6,while a whole W chromosome is constitutive heterochromatinization.Two NOR were observed on the pericentric of the No.7 chromosome.     

蝗虫染色体C带带型示意图的计算机绘制

该程序用C语言和FoxPro混合编写,把C语言的绘图优势和FoxPro强大的制表优势完美地结合起来, 通过输入实验测量的有关数据,可显示和打印出蝗虫C带带型的示意图,并可显示和打印出数据的统计表,以便于同模式图进行对照分析,并可将有关数据进行贮存。该程序稍作改动后可推广到其他动植物的C带以及其他带型示意图的绘制中。 Abstract:Using this program,we can draw all chromosomes of a species in line.Moreover,it can express centromere C-band,terminal C-band and internal C-band of each chromosome.This program not only can be used for locusts,after a little change,it can be used for all animals and plants.     

Construction of shRNA of Fulminant Hepatitis Related Gene mfgl2 and Investigation of Its Biological Effects in vitro

This study was designed to explore the RNA interference technique in inhibition of the expression of the mouse fibrinogen like protein 2(mfgl2),which has been reported to be involved in the development a variety of diseases including fulminant viral hepatitis.A plasmid named p-mfgl2shRNA,complementary to the sequence of mfgl2 was constructed,while another short hairpin RNA(shRNA) which was a mutated form of the mfgl2shRNA sequences was used as a control.A plasmid named pEGFP-mfgl2 expressing the mfgl2-EGFP fusion protein was also constructed for the screening of the effect of p-mfgl2shRNA on mfgl2 expression.By cotransfection of p-mfgl2shRNA and pEGFP-mfgl2 or pcDNA3.1-mfgl2 expression construct into CHO cells or HeLa cells,the inhibition of mfgl2 expression by mfgl2shRNA was analyzed by direct observation through fluorescent microscopy,FACS,RT-PCR and immunohistochemistry staining.The experiments showed the significant inhibitory effect of p-mfgl2shRNA on mfgl2 expression at 48h post-transfection in both CHO and Hela cell lines with the inhibitory efficiency as high as 80.1%.The study demonstrated that the construct of p-mfgl2shRNA successfully interfered with the mfgl2 expression in vitro.     

Grasshopper （Orthoptera： Acrididae） biodiversity and grassland ecosystems

Interesting results may arise by combining studies on the structure and function of ecosystems with that of biodiversity for certain species. Grasshopper biodiversity is the result of the evolution of grassland ecosystems; however, it also impacts on the structure and the function of those ecosystems. We consider there to be a close relationship between the health of grassland ecosystems and grasshopper biodiversity. The main problems involved in this relationship are likely to include： （i） grasshopper biodiversity and its spatial pattern; （ii） the effect of grasshopper biodiversity on the ecological processes of grassland ecosystems; （iii） the biodiversity threshold of grasshopper population explosions; （iv） the relationship between grasshopper biodiversity and the natural and human factors that affect grassland ecosystems; and （v） grasshopper biodiversity and the health of grassland ecosystems. The solutions to these problems may provide sound bases for controlling disasters caused by grasshoppers and managing grassland ecosystems in the west of China. In this paper, we introduced two concepts for grasshopper biodiversity, that is, ＂spatial pattern＂ and ＂biodiversity threshold＂. It is helpful to understand the action of the spatial pattern of grasshopper biodiversity on the ecological processes of grassland ecosystems and the effect of this spatial pattern on the health of those ecosystems, owing to the fact that, in the west of China, grasslands are vast and grasshoppers are widely distributed. Moreover, we inferred that the change in the level of component richness at each type of grasshopper biodiversity can make an impact on grassland ecosystems, and therefore, there is likely to be a threshold to grasshopper biodiversity for the stability and the sustainability of those ecosystems.     

菊花花部特征及花冠精油组分与访花昆虫的相关性

 菊花(Dendranthema morifolium)是异花授粉植物, 具有自交不亲和特性。其自然授粉的主要媒介为蜂类和蝶类。我们对19个菊花品种的花色、花朵繁密度、花冠直径、管状花花盘直径、株高以及花冠精油组分与访花蜂数和访花蝶数的相关性进行了研究。结果表明, 黄花色系较吸引西方蜜蜂(Apis mellifera)访花; 菊花的花朵繁密度、花冠直径、管状花花盘直径均与访花蜂数有显著相关性, 而株高与访花蜂数无相关性。大红蛱蝶(Vanessa indica)青睐红色花, 其访花蝶数与花朵繁密度、管状花花盘直径均有显著相关性, 而花冠直径和株高与访花蝶数无相关性。经气相色谱-质谱(GC-MS)分析得知, 不同菊花品种花冠精油的组分不同, 其主要成分均为单萜和倍半萜类及其含氧衍生物; 并且不同品种含有某些特定的花冠精油组分, 其中樟脑萜(r = –0.909, p<0.05)和6,10,14-三甲基-2-十五酮(r=0.882, p<0.05)与访花蜂数呈一定的相关性, α萜品醇(r=0.979, p<0.01)、顺柠檬烯氧化物(r=0.979, p<0.01)、金合欢烯氧化物(r=0.979, p<0.01)、p-mentha-6,8-dien-2-ol,cis- (r=0.973, p<0.01)、p-menth-1-en-8-ol (r=0.962, p<0.01)和4-萜品烯醇(r=0.957, p<0.05)与访花蝶数呈一定相关性。