红星凤梨的组织培养

植物名称:红星凤梨(Guzmania minor)。材料类别:嫩吸芽。培养条件:基本培养基为MS。(1)诱导愈伤组织培养基为MS BA0.5mg/L(单位下同) NAA0.5;(2)诱导丛生芽及增殖培养基为MS BA3 NAA0.2;(3)生根培养基为MS NAA0.5。温度25～28℃,日照12h,光照度1000～1500lx。     

PP333对油菜种间杂种试管苗生长发育的影响

甘蓝型油菜(Brassica napus)品种Willi与埃塞俄比亚芥(B.carinata)F_4代种子,白菜型油菜(B.campestris)品种郫县早黄油菜和七星剑作母本与埃塞俄比亚芥杂交授粉后胚发育到手杖期的不饱满种子,用75％乙醇处理30s,再用0.2％HgCl_2浸泡12min,无菌水冲洗3次。甘蓝型作母本的组合接种在1/2MS大量元素和1/2MS附加不同浓度的PP333的液体和固体等7种培养基上。     

感染苏云金杆菌后粘虫血淋巴中血细胞数量、蛋白质和酯酶的变化

用苏云金杆菌戈尔斯德变种的T84-9株和苏云金变种的7417株以亚致死剂量经口感染四龄末和五龄初的粘虫幼虫,观察其血淋巴中血细胞数、蛋白质和酯酶的变化。结果发现:(1)较高浓度的T84-9株处理组中,五龄初幼虫血细胞数明显下降,其他处理组与对照未见明显差别;(2)处理组五龄初幼虫的蛋白带比对照组多1条蛋白带,四龄末幼虫的蛋白带除染色深浅有差别外,未看到电泳带的明显变化;(3)较高浓度处理的四龄末幼虫比对照多1条酯酶带,较低浓度处理的四龄末幼虫比对照多2条带,五龄初幼虫的酯酶带未看到明显变化。     

评《光合作用——光子、激子、电子、质子、离子与光合膜之间的相互作用》一书

本书作者葛培根教授系美籍华裔学者,是国际上著名的光合作用专家,并享有很高的声誉。他长期在具有重要影响的光合作用研究中心 Kettering Research Laboratory 主持光合作用研究。光合作用研究的核心问题是光能转化的机理——光合作用的原初反应,即光子、激子、电子、质子、离子与光合膜之间的相互作用。这是光合作用研究中最富于挑战的领域。葛培根教授几十年来在这个研究领域中作出了杰出的贡献,推动了光合作用研究的发展。     

心脏中的Cl~-通道可被肾上腺素激活

关于阳离子在心脏电生理学的离子机制已有广泛报道。然而,最近日本Kyushu大学的两位学者T.Ehara和K.Ishhihara证明,心脏中的Cl~-通道可被肾上腺素激活,阴离子通道在交感活动控制中扮演一个特殊的角色。他们用电压钳技术记录到心肌细胞有一种外向的Cl~-通道整流电流。与心肌细胞中其它的儿茶酚胺依     

Gene transfer strategies in plants

In recent years, a large number of gene transfer methods have been developed. However, the results of these studies have often been published in such a way that it has been extremely difficult for researchers to assess the reliability and efficiency of the method, and to judge whether or not integrative transformation has occurred. Thus although an abundance of knowledge exists within the area of gene transfer, its documentation remains disjointed.This report summarises the recent progress which has been made in the field of gene transfer systems in plants and discusses the associated advantages, disadvantages and limitations in an attempt to clarify this issue.     

Aeroponics for the culture of organisms, tissues and cells

Characteristics of aeroponics are discussed. Contrast is made, where appropriate, with hydroponics and aero-hydroponics as applies to research and commercial applications of nutrient mist technology. Topics include whole plants, plant tissue cultures, cell and microbial cultures, and animal tissue cultures with regard to operational considerations (moisture, temperature, minerals, gaseous atmosphere) and design of apparati.     

Transport regulation of recombinant gene expression in E. coli and B. subtilis

Expression kinetics of the lactose (lac) operon in Escherichia coli are reviewed for both wild-type and recombinant cell cultures under chemostatic conditions. A unified model which involves regulation of active inducer (lactose) transport, promoter-operator regulated expression of the lac operon, glucose-mediated inducer exclusion, and catabolite repression is summarized and supporting data is shown to verify its accuracy. The synthesis of alpha-amylase with a recombinant form of Bacillus subtilis is also reviewed to point out generic features in transport regulation, the lac operon model providing a point of departure. While there are many similarities in the influence of transport on both regulating models, there are also important differences. In a chemostat system, the synthesis of alpha-amylase is nongrowth associated, while beta-galactosidase is a growth-associated enzyme. Nevertheless, transport regulation is an important feature in both instances.     

Enzyme stability in downstream processing. Part 1: enzyme inactivation, stability and stabilization

In biotechnological recovery processes the instability of the product can lead to large losses in the sequence of recovery processes needed to purify the product. As the cost of the final active product is strongly dependent on the recovery yield, this will lead to an increase in product cost. Therefore knowledge of factors that influence stability is important. This Part 1 contains a review on the factors that influence stability. As stability is very important in enzyme purification this review deals about enzymes and their ability to retain catalytic activity. Inactivation mechanisms and agents are discussed. A short review is given of enzyme structure and stability. This is followed by stabilization strategies and methods.