Development of Lateral-flow Immunoassay for WSSV with Polyclonal Antibodies Raised against Recombinant VP （19＋28） Fusion Protein

We have developed a sensitive and rapid lateral-flow immunoassay (LFIA) for WSSV, using colloidal gold as an indicator. The fusion protein, VP (19 28), was expressed in E. coli, purified and used to prepare polyclonal antibodies. The purified anti-VP (19 28) IgG were conjugated with colloidal gold. Unconjugated anti-VP (19 28) IgG and goat anti-rabbit IgG were immobilized on nitrocellulose membranes. After assembly, three groups (5 individual animals in each group) of shrimp samples were tested which included healthy, moribund and dead shrimps. For each group, three different tissues (body juices, gills and hepatopancreas) were tested at the same time. In parallel, all the samples were also analyzed using PCR for comparison. Out of 45 samples tested, 30 were detected as positive while 15 were classified as negative. The results of LFIA correlate with those obtained by the PCR analysis, indicating that these two detection methods have the same efficacy in the limited number of samples tested in this preliminary study.     

HFE C282Y homozygotes aged 25-29 years at HEIRS Study initial screening

We characterized HFE C282Y homozygotes aged 25-29 years in the HEmochromatosis and IRon Overload Screening (HEIRS) Study using health questionnaire responses, transferrin saturation (TfSat), serum ferritin (SF), and HFE genotyping. In eight homozygotes, we used denaturing high-performance liquid chromatography and sequencing to search for HFE2 (= HJV), TFR2, HAMP, SLC40A1 (= FPN1), and FTL mutations. Sixteen of 4,008 White or Hispanic participants aged 25-29 years had C282Y homozygosity (15 White, 1 Hispanic); 15 were previously undiagnosed. Eleven had elevated TfSat; nine had elevated SF. None reported iron overload-associated abnormalities. No deleterious non-HFE mutations were detected. The prevalence of C282Y homozygosity in White or Hispanic HEIRS Study participants aged 25-29 years did not differ significantly from the prevalence of C282Y homozygosity in older White or Hispanic HEIRS Study participants. The prevalences of reports of iron overload-associated abnormalities were not significantly different in these 16 C282Y homozygotes and in HFE wt/wt control participants aged 25-29 years who did not report having hemochromatosis or iron overload. We conclude that C282Y homozygotes aged 25-29 years diagnosed by screening infrequently report having iron overload-associated abnormalities, although some have elevated SF. Screening using an elevated TfSat criterion would fail to detect some C282Y homozygotes aged 25-29 years.     

植物细胞质膜离子通道研究进展

多种有机和无机离子作为重要的营养物质、渗透物质、辅酶和信号分子, 参与植物生殖、生长发育和逆境反应等多种生物学过程。离子通道是离子跨质膜和内膜运动的重要渠道和动态调控因子, 直接影响和调控细胞内离子浓度及亚细胞分布的动态变化。目前, 植物尤其是模式植物拟南芥(Arabidopsis thaliana)的多个离子通道家族被先后鉴定出来, 其中部分离子通道蛋白定位在细胞质膜上, 其基本生物学功能, 诸如蛋白结构、离子选择性和通透性、门控特点、活性调控机理以及不同离子通道之间的协同关系等均取得重要进展。该文概要介绍近年来植物细胞质膜离子通道方面的研究进展。     

虾青素功能米: 生物强化新思路, 优质米培育新资源

虾青素是一种红色的类酮胡萝卜素, 与其它类胡萝卜素或维生素E相比具有更强的抗氧化活性, 在延缓衰老、提高免疫力、防治糖尿病和心血管疾病等方面均有功效, 目前已广泛应用于制药、保健和日化等行业。但其合成必需基因BKT仅存在于部分微藻、细菌和酵母中, 大多数高等动、植物中并不存在。人类仅可以从三文鱼、贝类和鳟鱼等海洋生物中少量摄取虾青素。水稻(Oryza sativa)作为世界上最重要的粮食作物, 是全世界近1/2 (我国2/3)人口的主粮。稻米中缺乏类胡萝卜素前体, 因此无法自主合成虾青素, 且多年来在水稻中人工合成虾青素的尝试均未获得成功。近期, 中国科学家首次利用自创的多基因垛叠表达系统, 成功获得了富含β-胡萝卜素的黄金米, 富含角黄素的角黄素米和富含虾青素的赤晶米, 实现了从前体、中间产物到终产物的精准合成。     

注意缺损多动障碍关联于DXS7位点 Association of Attention-Deficit Hyperactivity Disorder and the DXS7 Locus

采用单体型相对风险和传递/不平衡检验方法,在中国人群中对注意缺损多动障碍（ADHD）与DXS7位点进行了遗传关联分析。结果表明,以父母双亲为对照,DXS7位点157bp等位基因与ADHD具有显著关联(χ2=9.10, P<0.05)并连锁(χ2=9.53, P<0.05)。提示,ADHD关联和连锁于DXS7位点。 Abstract　The present study was designed to assess the genetic association between attention-deficit hyperactivity disorder(ADHD) and DXD7 locus polymorphism in Chinese population, with haplotype-based haplotype relative risk(HHRR) and the transmission/disequilibrium test(TDT) analysis. We found significant association(χ2=9.10, P<0.05) and linkage (χ2=9.53, P<0.05) between the 157bp DXS7 allele and DSM-Ⅲ-R-diagnosed ADHD(N=54) in trios composed of father, mother and affected offspring. Our results suggest that ADHD was associated and in linkage with DXS7 locus.     

应用SSP-PCR/SSO方法进行中国辽宁汉族人HLA-DRB1基因的遗传多态性研究

对159名中国辽宁汉族个体的基因组DNA进行分析,共检出42种等位基因,其中以DRB1*09012(12.8%)、*0701(10.7%)、*1501(10.4%) 最为常见,其次为DRB1*1201(7.9%)、*1202(7.5%)、*1101(6.6%)、*0301(5.0%)。并发现辽宁汉族人DRB1等位基因频率与白种人间存在明显差异,揭示不同人种有其自己的主要等位基因。同时对本技术在HLA-DRB1分型应用中的优点进行了讨论。 Abstract　HLA-DRB1 alleles of 159 Chinese donors from Liaoning Han population were determined by using a set of 23 sequence specific oligonucleotide probes directed to various DRB1 alleles. The samples were first amplified and divided into 8 groups by allele/group specific primers. The SSOs enable us to identify 58 different DRB1subtypes. 42 alleles were detected in the study of this population. Among them, the DRB1*09012(12.8%), *0701(10.7%), *1501(10.4%), *1201(7.9%), *1202(7.5%), *1101(6.6%), *0301(5.0%) are the most frequent. The significant difference was found in Chinese northern Han population by comparing the gene frequencies in Caucasoid samples, suggesting that there were principal DRB1 alleles in different races.     

STOCHASTIC RESONANCE MEASURED BY THEMUTUAL INFORMATION IN THE NEURONSTHAT TRANSMIT CHAOTIC SPIKE TRAINS

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Retrovirus—mediated herpes simplex virus thymidine kinase gene therapy approach for hepatocellular carcinoma

INTRODUCTI0NHepatocellularcarcinoma(HCC)is0ne0fthem0stc0mm0nhumanmalignancies,causinganestimatedl,250,OOOdeatht0llperyearworldwide[1].Thep0orprognosisencounteredintreatment0fsuchcarcinomaismainlycausedbylatediagn0sisandinsufficiency0feffectivestrategies,especiallyforadvanced-stagedpatients.However,recentknowledge0fpathogenesisofHCCatm0lecularlevelprovidesanalternativeappr0achwhenc0nsideringgenetherapyastreatmelltf0rHCC.Am0ngthevari0usgenetherapystrategiesincancer)itwaJsrep0rtedthatth…     

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在45只切断双侧缓冲神经的Sprague-Dawley 大鼠,应用细胞外记录方法, 观察了颈动脉内注射腺苷对76 个最后区 (AP) 神经元自发放电活动的影响。所得结果如下(1) 在记录到的42个自发放电单位中, 颈动脉内注射腺苷(25 μg/kg) 引起其中29个单位的放电频率由6.26±0.75 下降至4.74±0.76 spikes/s (P<0.01), 6 个单位放电频率由4.13±0.77增加至4.72±0.83 spikes/s (P<0.05),另外7个单位放电频率无明显变化, 而血压和心率在实验中无变化; (2)在应用非选择性腺苷受体拮抗剂8-苯茶碱(8-phenyltheophylline, 15 μg/kg) 的10个单位, 腺苷对放电的抑制效应可被完全阻断; (3) 应用选择性腺苷A