研究方法达人

一次感冒激发了Stanley Fields应用于检测蛋白质相互作用的双杂交系统的发明。幸运的是，他对研究生物复杂系统新方法的热情并没有消退。     

广东省兴宁县红细胞葡糖-6-磷酸脱氢酶缺乏症的基因频率

遗传性红细胞葡萄糖-6一磷酸脱氢酶 (GOD）缺乏症在我国长江以南各省,尤其是 华南地区比较多见。它是导致伯氨哇嘛类药物 性溶血、蚕豆病、某些感染性溶血以及新生儿黄 疽的重要原因。了解此种致病基因在群体中的 分布,对溶血性疾病的防治和群体遗传学、遗传 流行病学以及人类学都有理论和实践意义。我 国广东、广西、云南、浙江、湖南、河南、四川、福 建、江苏、宁夏等省（区）某些地区都对本症的发 生率（incidence）进行过一些调查。但由于调查 对象的挑选不够严格,以及多采用间接反映 GOD活性的高铁血红蛋白还原试验筛选,故 难以准确计算本症的基因频率。我们于1980 年3月,采用了特异性较强的四氮哩蓝纸片法, 结合荧光斑法及四氮哩蓝定量法,对经严格挑 选的广东兴宁县居民和学生1,545人进行了 GOD缺乏症的群体普查,现将结果报告如下。     

Characterization and Phylogenetic Implications of the Complete Mitogenomes of Two Species in the Genus Zhangixalus(Anura: Rhacophoridae)

Mitochondrial genomes(mitogenomes) in frogs are essential for reconstructing the phylogenetic relationships and shedding light on the molecular evolution in these animals. However, there are only seven complete Rhacophoridae genomes that have been reported to date. In this paper, two complete mitogenomes of the Chinese whipping frog(Zhangixalus chenfui) and Emei tree frog(Z. omeimontis) were described, each of which was 20520 and 19782 bp in size, and had A+T contents of 64.26% and 63.83%.The tw...     

Inverse of Rensch's Rule of Allometry for Sexual Size Dimorphism in Bufo andrewsi

Difference in body size between males and females(sexual size dimorphism:SSD) and its variation are a common phenomenon in animal kingdom.Rensch’s rule predicts that the degree of SSD variation increases with the enlarged mean body size when males are larger than females and decreases when females are larger than males.Here,whether the patterns of variations in SSD in the Andrew’s toad(Bufo andrewsi)follow Rensch’s rule was tested using unpublished data from 14 populations and published data fro...     

A New Species of the Amolops mantzorum Group (Anura: Ranidae: Amolops) from Northwestern Hunan Province,China

We described anew species of the Amolops mantzorum group(Anura:Ranidae) from northwestern Hunan Province,China based on morphological and molecular evidence.The new species differs from its congeners by a combination of the following characteristics:moderate body size of SVL 403-409 mm in adult males(N=3),and 52.6-57.7 mm in adult females(N=3);head length about equal to or larger than head width(HDL/HDW 1.01-1.03);skin on dorsum smooth;dorsolateral folds absent,but developed a series of elongate...     

On the Validity of the Generic Nomen Liuixalus Li,Che, Bain,Zhao, and Zhang, 2008 (Anura: Rhacophoridae)

<正>Dear Editor,The genus Liuixalus Li, Che, Bain, Zhao, and Zhang, 2008 is a group of small-bodied Asian rhacophorid tree frogs and currently includes six species distributed in southern China and northern Vietnam(AmphibiaChina, 2022; Pham et al., 2018; Qin et al., 2015). The type species of Liuixalus, L. romeri(Smith, 1953), was originally assigned to the genus Chiromantis by Smith(1953) based on morphology. However, using molecular phylogenetic analysis, Li et al.(2008) found that C. rom...     

二化螟幼虫的田间分布

本文通过采用平均拥挤度的概念,运用动态分析方法,对二化螟(Chilo suppressalis WALKER)幼虫在稻田的空间格局进行了系统的研究.就聚集强度、格局纹理及动态的扩散过程进行了描述,综合地分析了各龄期幼虫空间格局的形成与演变,并揭示了幼虫种群在稻田的分布特征及分布形成的内在过程,明确了二化螟幼虫空间分布研究在生态学及害虫管理上的意义.     

中华蜜蜂工蜂触角感受器的扫描电镜观察

对中华蜜蜂(Apis cerana)工蜂触角感受器的扫描电镜观察,见到在触角上有九种类型的感受器,它们是板形感器、腔锥感器、坛形感器、钟形感器、锥形感器、毛形感器A、毛形感器B、毛形感器C和D、缘感器以及各种类型的刚毛等.对于这些感受器的外部形态和分布部位进行了详细地观察和描述,发现中华蜜蜂与西方意蜂(Apis mellifera)有差异.     

胰腺的病理生理学

本文主要论述胰腺的外分泌并着重于胰腺的炎症。在阐明胰腺炎发病学方面以及改善胰腺诊断方面的进步,使这一疾病症状表现的机制得以相当明了,并使治疗变得更加合理而有效。胰腺的生理学我们对胰腺生理学的知识是在研究了下列问题后获得的:胰腺外分泌及其调节机制;胆管和胰腺导管系统的解剖学和生理学;胰腺的蛋白质代谢以及胰腺导管压力改变对胰腺刺激所引起的反应。胰腺的外分泌胰液是一种主要由重碳酸