云南绵羊线粒体DNA遗传多态性研究

本研究用12种识别六碱基的限制性内切酶对来自云南省3个地方绵羊品种11个样本的线粒体DNA(mtDNA)进行了限制性片段长度多态性分析(RFLP),结果表明,云南绵羊群体平均核苷歧异度为0.086%,平均遗传距离为0.00 4,说明mtDNA变异度很低,遗传多样性贫乏,提示云南绵羊可能起源于同一个共同祖先;昭通绵羊与德钦绵羊距离较远,大约分歧于30万年前,德钦绵羊与腾冲绵羊大约分歧于11万年前,而腾冲绵羊与昭通绵羊也于18万年前发生分歧。 Abstract:mtDNA from 11 sheeps samples of Deqin,Zhaotong and Tengchong in Yunnan province was analyzed with 12 restriction endonucleases.14 restriction types were observed which could sorted into 3 haplotypes.The estimated number of nucleotide substitutions per site(π)in Yunnan sheep was 0.086% and the mean genetic distance(P) was 0.004.This results might show a pattern of single origin of Yunnan sheep.Divergencetime of the native sheep flocks from three districts of Yunnan were estimated on the mean rate of sequence divergence of 0.01 per million years in mtDNA.     

裂果易发性不同的荔枝品种果皮中细胞壁代谢酶活性的比较

“糯米糍”荔枝裂果率极显著高于“淮枝”,前者果皮中的果胶酶、纤维素酶和果胶甲酯酶的活性高于后者,其中以果胶酶活性差异最明显,其次是纤维素酶,果胶甲酯酶差异最小;“糯米糍”细胞壁结合型的过氧化物酶(POD)和多酚氧化酶(PP0)活性明显高于“淮枝”,而水溶性POD和PP0的活性则两个品种间无明显差异。据此认为,果皮细胞壁水解酶活性以及细胞壁结合型的POD和PPO的活性高的荔枝品种,其裂果率也高。文章对细胞壁代谢相关酶类在果皮抗裂性形成中的作用进行了讨论。     

短暂脑缺血大鼠海马CA1区锥体细胞大电导Ca2+依赖K+通道活动降低

短暂脑缺血可对随后的损伤性脑缺血表现出明显的耐受.有研究表明大电导Ca2+依赖K+(BKCa)通道活动增强参与了缺血性脑损伤.采用膜片钳的内面向外式,观察了3 min短暂脑缺血后6 h、24 h以及48 h大鼠海马CA1区锥体细胞上BKCa通道活动的动态变化.短暂脑缺血后BKCa通道的单通道电导和翻转电位均未见明显变化,但通道的开放概率则在缺血预处理后的前24 h内显著降低.通道动力学分析显示通道关闭时间变长是短暂脑缺血后通道活动降低的主要原因,因为通道的开放时间未发生明显变化.结果提示短暂脑缺血所致的BKCa通道活动降低可能与缺血耐受的产生有关.     

Projects Funded by the Division of Botany （NSFC） in 2005： Retrospect and Prospect

The present paper briefly introduces information about proposals received and funded, statistics and analysis of evaluation of peer review, and supporting strategies and reforms in program reviewing and administration in the Division of Botany at the Natural Science Foundation of China （NSFC） in 2005. A list of general programs and the abstract of key programs funded by this Division in 2005 are also provided.     

棉属A染色体组的核型研究

本文报道了棉属A染色体组3个种(变种)的核型,阿非利加棉和草棉的核型公式均为20m+4sm(2sat)+2st(2sat),亚洲棉的核型公式为22m+2sm(2sa t)+2st(2 sat)。平均臂比和染色体长度比分别是:阿非利加棉1.63和1. 73,草棉1.87和1.74,亚洲棉1.58和1.62。其随体均大而明显,数目和位置相同。3个种(变种)的核型同质性与其分类学上同归于A染色体组是一致的。阿非利加棉和草棉的亲缘关系比二者与亚洲棉的密切,但它们存在核型构成的差异,这些差异同时也证实了系统演化史上阿非利加棉先于草棉的论点。     

Distribution of T-DNA carrying a Ds element on rice chromosomes

Rice is one of the most important crops in the world, and is widely studied as a model for cereal ge-nomics because of its small genome size (about 430 Mbp), and its colinearity at the sequence level with limited regions of other cereal genomes. In addition, there are a large number of rice databases document-ing molecular markers, genome sequences, EST se-quences and trait mutants[1—4]. Functional genomic studies of rice are increasing with the availability of the complete genome sequence. …     

欧亚大陆新近纪气候演化(NECLIME)研究进展与展望

"欧亚大陆新近纪气候演化"国际研究团体成立于2000年,其前期工作主要集中在欧洲。随着研究的不断深入,亚洲地区的工作开始受到越来越多的关注。2010年11月在昆明举办了首届亚洲NECLIME年会,今年5月下旬又在南京成功召开了NECLIME研讨会,讨论的内容也有了更多的拓展。为使国内相关研究人员对这一国际研究团体及其目标有更多的了解,本文拟对NECLIME的发展史、研究进展和南京会议作一简要介绍,并对NECLIME的今后工作进行展望,以期对国内的相关研究提供帮助。     

Construction of shRNA of Fulminant Hepatitis Related Gene mfgl2 and Investigation of Its Biological Effects in vitro

This study was designed to explore the RNA interference technique in inhibition of the expression of the mouse fibrinogen like protein 2(mfgl2),which has been reported to be involved in the development a variety of diseases including fulminant viral hepatitis.A plasmid named p-mfgl2shRNA,complementary to the sequence of mfgl2 was constructed,while another short hairpin RNA(shRNA) which was a mutated form of the mfgl2shRNA sequences was used as a control.A plasmid named pEGFP-mfgl2 expressing the mfgl2-EGFP fusion protein was also constructed for the screening of the effect of p-mfgl2shRNA on mfgl2 expression.By cotransfection of p-mfgl2shRNA and pEGFP-mfgl2 or pcDNA3.1-mfgl2 expression construct into CHO cells or HeLa cells,the inhibition of mfgl2 expression by mfgl2shRNA was analyzed by direct observation through fluorescent microscopy,FACS,RT-PCR and immunohistochemistry staining.The experiments showed the significant inhibitory effect of p-mfgl2shRNA on mfgl2 expression at 48h post-transfection in both CHO and Hela cell lines with the inhibitory efficiency as high as 80.1%.The study demonstrated that the construct of p-mfgl2shRNA successfully interfered with the mfgl2 expression in vitro.     

慢病毒GFP质粒的构建及脂质体介导鸡胚细胞中瞬时表达动力学初步研究

GFP(green fluorescent protein)是一种用于检测细胞的基因表达和蛋白定位的报告基因,pLenti6/V5-D-TOPO是高效的慢病毒类表达载体.实验利用PCR(polymerase chain reaction)从质粒pEGFP-N1中扩增了EGFP 基因片段,再利用高效、快速、定向的TOPO克隆法将扩增片段克隆到pLenti6/ V5-D-TOPO载体中,构建了既能高效转染细胞又能方便观察蛋白表达情况的pLEGFP重组载体.通过PCR法鉴定及瞬时转染,结果显示,GFP基因正确地插入载体中,并能够在Hela细胞及鸡胚X期细胞中瞬时表达,但重组和对照质粒瞬时转染效果存在明显差异.通过该研究证实分子量大小、质粒和脂质体比例是影响转染的关键因素,也为转基因的研究提供了一种方法.