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pms3 is the locus causing the original photoperiod-sensitive male sterility mutation of‘Nongken 58S' 总被引:8,自引:0,他引:8
Photoperiod-sensitive genic male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonica cultivar 'Nongken 58'. pms3 on chromosome 12 was determined to be the locus where the original PSGMS mutation occurred, changing the normal cultivar Nongken 58 to PSGMS Nongken 58S. Large amounts of RAPD and AFLP analyses were also conducted for the fine mapping of the pms3 genomic region, which resulted in 4 molecular markers linked to pms3. Although these markers somewhat increased the marker density of this region, the pms3 locus is still located in a marker-sparse region. 相似文献
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植物的功能基因组学研究进展 总被引:38,自引:1,他引:38
基因组研究计划包括以全基因组测序为目标的结构基因组学和以基因功能鉴定为目标的功能基因组学两方面的内容。目前基因功能鉴定的方法主要有:基因表达的系统分析(SAGE)、cDNA微阵列、DNA(基因)芯片、蛋白组技术以及基于转座子标签和T_DNA标签的反求遗传学技术等。本文对上述各种技术的优缺点以及它们在植物基因功能鉴定中的应用进行了综述。
Abstract: The genome projects comprise the structural genomics focusing on determining the complete sequences of the genome and the functional genomics focusing on elucidating the biological function of genes.The rapidly evolving tools for functional genomics research include Serial Analysis of Gene Expression (SAGE),cDNA microarray,DNA (or gene) chips,proteome project and the reverse genetics technique based on the well-established transposon tagging and T?DNA tagging systems.In this paper,the advantages and disadvantages of such techniques and application of these techniques in plant functional genomics research are reviewed and future prospective are also presented. 相似文献
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RFLP、RAPD、AFLP在水稻农垦58S和1514中多态性比较 总被引:8,自引:1,他引:7
本文用RFLP、RAPD和AFLP三种分子标记技术对农垦58SX1514组合及其F2极性集团进行了分析,比较了它们多成性和阳性的比率,结果显示,三种分子标记的多态性和与目的基因连锁的阳性比率分别为19.93%,5.23%;11.17%,0.76%和86.47%,7.52%。AFLP的多态性比率和阳性比率均为最高。分析探讨了三种分子标记技术的优缺点及其在区间高分辩率作图和筛选与目的基因连锁标记中的运 相似文献
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环境因子胁迫下水稻翻译延伸因子1A基因的诱导表达 总被引:4,自引:0,他引:4
利用差异显示PCR 技术(DD_PCR) 比较了籼稻( Oryzasativa L.ssp.indica)“窄叶青8 号”幼苗在盐胁迫与正常生长条件下基因表达的差异,克隆了水稻翻译延伸因子1A 蛋白(eEF1A) 基因家族中的一个新的成员( 称为REF1A) 。利用Northern 杂交对环境因子胁迫下该基因的表达进行了分析,结果翻译延伸因子1A 基因在水稻幼苗中的表达明显受盐胁迫和ABA胁迫所诱导,且ABA 胁迫处理对该基因转录的诱导要早于盐胁迫的诱导作用。此外,干旱(15% PEG6000) 处理、冷胁迫(4 ℃)和热激(37 ℃) 对水稻翻译延伸因子1A 基因的转录均有明显的诱导作用。上述结果说明翻译延伸因子1A基因的诱导表达可能是水稻细胞对逆境胁迫的一种适应性反应 相似文献
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