口红吊兰衰老过程中整体与部分被遮挡叶片超微弱发光比较

以口红吊兰叶片为试材,用生物超微弱发光探测技术研究衰老过程中整体叶片与部分被遮挡叶片延迟发光的变化.结果表明:光照整体叶片与光照部分叶片延迟发光的曲线平行,而整体叶片与被遮挡部分叶片延迟发光的规律不同;幼叶与老叶整体延迟发光衰减快,被遮挡部分延迟发光衰减慢;成熟叶片整体延迟发光衰减慢,而被遮挡部分的延迟发光衰减快.研究认为:在同一外部条件下,整体叶片与被遮挡部分叶片延迟发光变化规律不同,植物叶片的这种发光现象可能在生物系统内部有自己独特的规律.     

全反式维甲酸联合辛二酰苯胺异羟肟酸诱导MCF-7 细胞凋亡

目的:探讨全反式维甲酸(ATRA)和辛二酰苯胺异羟肟酸(SAHA)单独及联合应用对乳腺癌MCF-7细胞凋亡的影响。方法:培养人乳腺癌MCF-7细胞,待细胞进入对数生长期,加入一定浓度的ATRA和(或)SAHA,采用流式细胞术检测细胞的凋亡情况。结果:ATRA和SAHA单药及联合应用早期凋亡率均高于对照组,但24 h时测得联合组早期凋亡率低于单药组,48 h、72 h联合组早期凋亡率高于SAHA组却低于ATRA组,差异均有统计学意义(P0.05);联合组死亡细胞和晚期凋亡细胞率之和高于单药组及对照组,差异有统计学意义(P0.05)。结论:ATRA和SAHA单独或联合作用于乳腺癌MCF-7细胞均可促进细胞凋亡,两药联合应用时诱导细胞凋亡作用更强。     

Raman microspectroscopic study of biomolecular structure inside living adhesive cells

Cells adhesion is very important for many physiological processes. Using advanced Raman microspectroscopic technique, we selected T Leukemia cells (Jurkat) as the materials and obtained simultaneously conformation information of various biomolecules inside the whole living cells. By comparing the Raman microspectroscopic spectra of single and adhesive cancer cells, we found for the first time that when cells adhered, the conformation of the biomolecules (DNA, protein, carbohydrates and lipids) inside the cells had different changes: (i) the backbone of double-stranded DNA maintained orderly B-form or modified B-form conformation, whereas the groups of its deoxyribose and bases were modified; (ii) the conformational changes of the main chain and the side chain in the protein were obviously variant. The lines intensity belonging to α-helix and β-sheet decreased, while that of β-turn increased. Tyrosine and tryptophane residues of the protein changed from “buried state” to “exposed state”; the lines intensity of its sulfhydryl group also increased; the conformation of its disulfide bond changed from two kinds to three kinds. These facts suggest that the cells adhesion causes changes in H-bonds organization of the main chain and environment of the side chain in the protein; (iii) the groups of the carbohydrates were also modified simultaneously; (iv) the conformation of the lipids bilayers of the membranes changed obviously; the order parameter for lateral interaction between chains decreased gradually with the increase of number of the adhesive cells. So cells adhesion resulted in an increase in fluidity of the membrane and ion permeability on the membrane.     

心理咨询对血友病患者心境障碍的干预效果

目的:观察心理咨询对血友病心境障碍患者的干预效果。方法:选择2014年1月~2015年4月在哈尔滨医科大学附属第四医院血液内科接受治疗的血友病患者63例,年龄从18~55岁且具有良好的思维能力和判断能力,并获得知情同意的,用心理咨询的方法前后用汉密尔顿焦虑量表和汉密尔顿抑郁量表进行测查,得出结论。结果:在对63例血友病的患者心理咨询及测查的结果看,血友病患者中有抑郁情绪的比率从88.9%降低到63.5%,抑郁情绪的平均值从17.62降到9.98差异显著。有焦虑情绪的比率从77.8%降低到57.1%,平均值从20.87降到12.06差异显著。具有统计学意义,这充分说明了心理咨询对血友病患者是有效果的。结论:用心理咨询的方法对血友病患者进行心理干预是有效的。     

植物生长调节剂对花叶开唇兰原球茎增殖和分化的影响

应用正交设计法探讨3种植物生长调节剂对花叶开唇兰原球茎增殖和分化的影响。结果表明,6-BA对原球茎增殖和分化作用显著,NAA和KT作用不显著;原球茎增殖最佳培养基为改良KC+6-BA 2.0 mg/L+NAA 1.5 mg/L+KT 0.2 mg/L,原球茎分化最佳培养基为改良KC+6-BA 1.0 mg/L+NAA 0.5 mg/L。     

从HFRS患者的PBMC建立BLCL及其意义

体外研究汉滩病毒(Hantaan virus,HTNV)S基因及其5’端、3’端在BLCL(EBV-transformed B lymphoblastoid cell line,BLCL)内稳定表达的意义,为核蛋白T细胞表位的研究奠定基础。本研究从肾综合征出血热(HFRS)患者的外周血单个核细胞(PBMC)建立与HTNV特异性CTL同源的患者自身的EB病毒转化的B淋巴母细胞系,将含有不同HTNVS基因片段的重组真核表达载体转染入BLCL,获得长期稳定表达,作为靶细胞系,为下～步进行CTL杀伤试验提供靶细胞系。设计4条引物,用PCR方法从PBV220-S22原核质粒中扩增出s基因全读码框(37—1326bp)及s基因5’端(37—501bp),S基因3’端(502—1326bp),用TA克隆将其克隆入pcDNA3．1／V5．His-TOPO载体中,成功构建pcDNA3．1-S及pcDNA3．1-S—N、pcDNA3．1-S-C真核表达载体,并通过脂质体转染至BLCL细胞中,进行了稳定表达。间接免疫荧光成功检测到pcDNA3．1-S及pcDNA3．1-S-N、pcDNA3．1-S．C在BLCL细胞中的表达。pcDNA3．1-S及pcDNA3．1-S—N、pcDNA3．1一S—C真核表达载体有较高的转染效率,目的基因能在宿主细胞中长期稳定表达,有利于研究HTNV-S基因在T细胞表位研究中的意义。建立的特异性CTL克隆对表达完整NP、NP羧基和氨基端肽段的靶细胞均有比较明显的杀伤效应,平均杀伤率分别为50．2％、39．0％和25-4％。HTNV-NP优势T细胞表位可能主要位于病毒核蛋白的羧基端。     

电镜观察黄芪对豚鼠I型超敏反应早期反应和晚期反应的治疗作用

目的:研究黄芪对豚鼠Ⅰ型超敏反应早期反应和晚期反应超微结构的影响.方法:马血清致敏豚鼠1周后,黄芪粉稀释液(2.7×10-2g/kg体重)胃管给药.2周后发敏,出现典型发敏症状者立即麻醉,取十二指肠下段及肺组织作早期反应材料,发敏后5 h仍存活者,取上述同样组织做晚期反应材料.结果:通过透射电镜(TEM)超微结构的观察发现,Ⅰ型超敏反应早期反应中即有典型的平滑肌收缩,腺体分必增加,毛细血管扩张等功能性障碍,还伴有线粒体肿胀,嵴断裂、空泡性变;肌丝溶解、空泡变;小肠上皮细胞微绒毛肿胀,断裂、丢失等现象;同时早期反应的肠粘膜固有层中有较多的凋亡细胞存在.晚期反应中除有嗜酸粒细胞浸润的炎症外,尚有少量纤维渗出及Ⅱ型肺泡细胞的增生,可见Ⅱ型肺泡细胞的的凋亡.黄芪能明显抑制Ⅰ型超敏反应的上述早期反应及晚期反应.结论:Ⅰ型超敏反应的早期反应除有功能障碍外,还有器质性损伤(坏死和凋亡两种形式);黄芪对豚鼠Ⅰ型超敏反应有显著治疗作用.     

The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg

The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured and used as nucleus donor for the construction of interspecies embryos by transferring them into enucleated rabbit eggs. All the three kinds of somatic cells were able to reprogram in rabbit ooplasm and support early embryo development, of which mammary gland cells were proven to be the best, followed by uterus epithelial cells and skeletal muscle cells. The experiments showed that direct injection of mammary gland cell into enucleated rabbit ooplasm, combined with in vivo development in ligated rabbit oviduct, achieved higher blastoeyst development than in vitro culture after the somatic cell was injected into the perivitelline space and fused with the enucleated egg by electrical stimulation. The chromosome analysis demonstrated that the genetic materials in reconstructed blastocyst cells were the same as that in panda somatic cells. In addition, giant panda mitochondrial DNA (     

牛肌肉生长抑制素基因突变的遗传效应与育种应用

肌肉生长抑制素（myostatin,MSTN）基因主要在骨骼肌中表达,参与调控骨骼肌的生长发育。MSTN基因在不同物种中具有极强的进化保守性,同时还具有较多的突变多态性。在牛的不同品种中,存在不同位点的有义突变,突变型牛均表现为骨骼肌发达,呈现双肌表型,生长速度与产肉率显著提高。同时,该基因突变也引起显著的生理性遗传效应。对国内外肉牛的MSTN基因突变类型、突变后遗传效应及在肉牛育种应用等方面作了重点阐述,以期为我国地方品种肉牛改良和选育研究提供参考。