通过PEG法转化甘蓝获得转基因植株

通过PEG法将外源基因导入甘蓝(Brassica oleracea L.)下胚轴原生质体。以潮霉素和卡那霉素进行筛选,均成功地得到了抗性愈伤组织。对于潮霉素抗性愈伤组织,其绝对和相对转化频率分别为4.1×10~(-4)和1％～3％,在分化培养基上,抗性愈伤组织能够分化出芽。诱导生根后进行移栽,生长状况良好。Southem blotting分析表明外源基因已插入到植物细胞基因组中。对影响转化的一些因素进行了探讨。     

Effects of specific expression of iaaL gene in tobacco tapetum on pollen embryogenesis

The indoleacetic-acid-lysine synthetase (iaaL) gene from Pseudomonas syringae subsp. savastanoi was fused to tobacco tapetum-specific expression promoter TA29, and introduced into tobacco. The expression pattern of this chimeric gene was studied, and the endogenous indoleacetic acid (IAA) levels in different organs were assayed. The results demonstrated that TA29 promoter was only able to direct the specific expression of iaaL gene in transgenic tobacco anther, and resulted in the decrease of endogenous IAA levels in transgenic tobacco anther. No significant phe-notype variation was observed among the transgenic plants at the whole plant level. However, the percentage of pollen embryogenesis was reduced to 11 % when anthers of the transgenic plants were cultured on the modified hormone-free Nistch H (NH) medium, while those of both CK1 and CK2 (see sec. 1.2.2) were more than 50% ; when the an-thers were cultured on NH medium supplemented with 0. 2 mg/L IAA, the percentage of pollen embryogenesis re-stor     

几种念珠菌DNA总含量的流式细胞分析

应用流式细胞术(FCM)对处于稳定生长阶段的念珠菌属(Candida)的7种8株念珠菌进行了DNA总含量的流式细胞(FCM)分析。这8株念珠菌是:白念珠菌(C.albicans)2株,热带念珠菌(C.tropicalis),克柔念珠菌(C.krusei),近平滑念珠菌(C.parapsiolosis),乳酒念珠菌(C.kefyr),白念珠菌星形变种(C.stellatoidea),即血清B型白念珠菌,季也蒙念珠菌(C.guilliermondii)各一株。应用EB一步插入法染色,用鸡红细胞(CRBC)作为内参标准进行DNA总含量测定。分析结果表明:稳定生长阶段的组方图上,大多数念珠菌细胞处于DNA合成周期的G_0/G_1期;DNA总含量有明显的种间和种内差异。     

消灭传染病的国际协作（续三）儿童疫苗的构思

消灭传染病的国际协作（续三）儿童疫苗的构思大谷明（日本国立预防卫生研究所名誉所员）儿童疫苗构思的原委１９７４年,世界卫生组织（ＷＨＱ）采纳了扩大预防接种计划（ＥＰＩ）,指定对白喉、百日咳、破伤风、结核、麻疹及脊髓灰质炎等６种疾病推行到１９９０年全世界...     

瓠瓜的离体培养和植株的高效再生

９￣１０ｄ苗龄的瓠瓜无菌苗的带叶基（子叶片与子叶柄之间的部分）子叶在８种分化培养基上培养１５ｄ左右,结果在含３．５ｍｇ／Ｌ ＺＴ的培养基上能以较高的频率直接分化出芽;分化培养前对带叶基子叶及仅含子叶叶基的两种外植体纵向切割都能明显提高出芽率,不含子叶叶基的外植体不能分化出芽,决定出芽的外植体的部位是子叶叶基;纵向切割子叶叶基前预培养９ｄ以上出芽率大幅度提高。     

峨眉尖舌苣苔（苦苣苔科）花部形态发生及其系统学意义

观察了峨眉尖舌苣苔（Ｒｈｙｎｃｈｏｇｌｏｓｓｕｍｏｍｅｉｅｓｅ）花序及花的形态过程,并同近缘类群进行比较和分析,结论如下：１）峨眉大苣台花发生组织通常的总状花序分生组织已发生开变和位偏从而导致不平衡分化,形成花偏向于腹部一侧的变了形的总状花序,２）花冠和能育雄蕊的两侧性可能和退化雄蕊相关。３）该种雄蕊早期的发生和发育过程仍保持着祖先群群中轴肥座的系统发生式样,并在随后的发育过程中转变成侧膜胎座,４     

海南岛堆砂白蚁属的两个国内新纪录种

长颚堆砂白蚁 Cryptotermes dudleyi Bonks 分布 广东省海南岛(崖县、澄迈县);菲律宾,马来西亚,印度尼西亚,斯里兰卡,印度,新几内亚,澳大利亚,加勒比海沿岸,中南美,非洲。 兵蚁(图1,1—5)头前部深栗褐色,由额脊向后渐淡。头部略呈长方形。额区外截呈铲面状,其后缘成两宽弧形脊状隆起;在头顶中相切处成浅或深的中断。头盖缝Y状,     

鼎湖山散白蚁属的一新种(等翅目:犀白蚁科)

鼎湖散白蚊Reticulitermes dinghuensis,新种 兵族(图1—6,表1)