Adsorption and adhesion of blood proteins and fibroblasts on multi-wall carbon nanotubes

This article concerns the investigation of blood protein adsorption on carbon paper and multi-wall carbon nanotubes (MWCNTs). Mouse fibroblast cell adhesion and growth on MWCNTs was also studied. The results showed that fibrinogen adsorption on carbon paper was much lower than that on MWCNTs, which means that platelets readily aggregate on the surface of MWCNTs. Mouse fibroblast cells implanted on MWCNTs tended to grow more prolifically than those implanted on carbon paper. The cell concentration observed on MWCNTs increased from 1.2×10⁵/mL for a single day culture to 2×10⁵/mL for a 7-day culture. No toxicity reaction was observed during the culturing period. These results indicated that MWCNTs possessed excellent tissue compatibility. Supported by open-program of State Key Laboratory of Bioelectronics, Southeast University.     

New Species of Meconopsis (Papaveraceae) from Mianning,Southwestern Sichuan

Two new species of Meconopsis, Mpulchella and Mheterandra are described and characterized. Differences with similar species are discussed and photos showing the diagnostic features are provided.     

不同钙盐对温州蜜柑抗冻性的影响

用20 mmol·L^-1氯化钙（CaCl₂）、醋酸钙（CaAc₂）和硝酸钙（Ca(NO₃)₂）喷施“国庆1号”温州蜜柑叶片,研究3种钙盐对其叶片抗冻性的影响.结果表明：喷施钙盐降低了叶片半致死温度（LT₅₀）,提高了温州蜜柑的抗冻性,CaCl₂处理的LT₅₀比对照降低了0.54 ℃,而CaAc₂和Ca(NO₃)₂处理分别比对照降低了1.34 ℃和1.35 ℃,表明CaCl₂处理效果较差,CaAc₂和Ca(NO₃)₂处理效果较好.钙盐处理还提高了“国庆1号”温州蜜柑叶片在低温（-9 ℃）胁迫下的SOD、POD活性及可溶性蛋白质、可溶性糖和脯氨酸含量,减少了叶片MDA的积累.     

A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation

p28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13sucl-agarose affinity chromatography. Sequence homology analysis of the full-length cDNA of p28 (Gene Bank accession number: AF 314091) indicated that it encodes a protein containing 224 amino-acids with about 55% identities and more than 70% positives to human, rat or mouse UCH-L1, and contains homological functional domains of UCH family. Anti-p28 monoclonal antibody, on injecting into the oocytes, could inhibit the progesterone-induced resumption of meiotic division in a dose-dependent manner. The recombinant protein p28 showed similar SDS/PAGE behaviors to the native one, and promoted ubiquitin ethyl ester hydrolysis, a classical catalytic reaction for ubiquitin carboxyl terminal hydrolases (UCHs). The results in this paper reveal that a novel protein, p28, exists in the toad oocytes, is a UCH L1 homolog, was engaged in the process of progesterone-induced oocyte maturation possibly through an involvement in prot     

大鼠早期三级卵泡中的卵母细胞能在联合培养系统中成批适当成熟

我们建立了由离体同步未分化颗粒细胞、动情前期垂体前叶和Δ-4雄烯二酮组成的“联合培养系统”。该联合培养系统可模拟正常生理状态下各相关细胞间的协同作用,更有效地诱发经DES 刺激后所获得的大量大鼠早期三级卵泡中的卵母细胞同步达到适当成熟,从而获得符合正常生理标准的成熟卵球,使卵巢内大量卵母细胞得到充分利用。我们的实验表明,经DES刺激,每侧幼龄大鼠卵巢中平均可取得处在早期三级卵泡中的189枚卵母细胞。在联合培养系统中培养,78%的卵母细胞排出第一极体达到成熟。这些成熟的卵母细胞中88%可正常受精,93%卵裂,经体外培养96h, 有59%的受精卵可发育到桑椹或囊胚。2-细胞期胚胎移植后可发育为健康个体。因此,该联合培养系统为研究大鼠卵母细胞成熟、受精和早胚发育的分子机制提供了非常有效的实验模型。     

Effect of the infectious laryngotracheitis virus (ILTV) glycoprotein G on virus attachment, penetration, growth curve and direct cell-to-cell spread

Glycoprotein G plays many important roles in al-phaherpesvirus except for the varicella-zoster virus (VZV), from which gG is absent[1]. The gG of most alphaherpesviruses is secreted after proteolytic proc-essing, as occurs in the herpes simplex virus 2 (HSV- 2)[2―4], bovine herpesvirus 1 (BHV-1)[5―7], bovine herpesvirus 5 (BHV-5)[8], equine herpesvirus 1 (EHV- 1)[9], equine herpesvirus 3 (EHV-3)[10], equine herpes-virus 4 (EHV-4)[11], feline herpesvirus 1 (FHV-1)[12], infectious la…     

中国隼姬蜂属一新种(膜翅目：姬蜂科)

记述采自河南省内乡县宝天曼自然保护区的姬蜂科Ichneumonidae一新种;斜隼姬蜂Neliopisthus inclivatus sp.nov.。本种与雅住址 N.elegans(Ruthe）相似,主要区别特征如下,斜住址姬蜂：触角比前长;触角鞭节30－34节;侧单眼长径（♂）为单复眼间距的0.3-0.4倍。后小脉强烈内斜,在接近下端（0.13）处曲折。雅隼姬蜂：触角不比翅长;触角鞭节不多于28节;侧单眼开径（♂）约为单复眼间距的0.91倍;后小脉仅上段内斜,在中央稍下方曲折。     

成骨不全显性遗传一家系五代28例报告

对成骨不全先证者上溯5代372人进行家系调查,发现该家系共有28例成骨不全,其中已死亡10例(成人、儿童各5例),均非死于本病;现生存18例(成人12例、儿童6例)。该28例均身材矮小、身高显著低于同龄正常者;均有蓝色巩膜;符合常染色体显性遗传缺陷的结缔组织病。现存活18例中,耳聋、听力下降6例;骨折9例(22例次),对照文献中分型标准,本组28例与Ⅰ型、Ⅲ型的部分临床特征相符,是否系介于Ⅰ型、Ⅲ型之间的亚型,有待于进一步研究。 Abstract:A pedigree investigation was carried out on 372 relatives in five generations of a proband of osteogenesis imperfecta.The results showed that there were 28 patients in the family.10 of them died from other causes(5 adults and 5 children respectively).Other 18 cases are alive(12 adults and 6 children respectively).The height of the 28 cases were significantly lower than those of the same age.Furthermore,they all had blue scleras.Their clinical manifestations conformed to those of autosomal dominant hereditary connective tissue disease.Among the living 18 cases,6 suffered from deafness or hearing loss and 0 suffered from fracture(22 person-time altogether).According to the classification criteria in the literature,the clinical manifestations of the 28 cases conformed to those of type I and type III,It is necessary to study further whether they belonged to the subtype between type I and type III.     

植物雄性不育的分子基础

不能产生功能花粉粒的植物称为雄性不育植物。Kolreuter早在1763年就观察到该现象。达尔文1890年也作过报道。后来的研究工作者分别对欧洲夏季薄荷、甜菜、玉米、高粱、小麦等作物的雄性不育现象展开了较系统的研究,为解释植物雄性不育现象奠定了遗传学、细胞学、生理生化学的基础、而对其分子基础的研究在很大程度上取决于分子生物学技术的发展。近十几年来,由于细胞器DNA分离研究技术的涌现,限制性内切酶技术