胡杨花粉辐射杂交可配性与杂种选育

本研究通过花粉辐射, 提高胡杨与其它杨树杂交的可配性,并获得箭胡毛杨P. thevestena×(P. euphratica+P. tomentosa ev.)和卢胡毛杨P.euramericana×(P.euphratica+P.tomentosa ev.)的人工杂种,筛选出的优良无性系已在河西走廊试种推广。 Abstract:The study was designed to increase the cross-compatibility between Euphrates Poplar and other poplars with pollens radiated byγ-ray.The artificial hybrids from P.thevestena×(P.euphratica+P.tomentosa) and P.euramericana×(P.euphratica+P.tomentosa) have been obtained.The selected good clones have been planted and spread in Hexi Corridor area of Gansu Province.     

Molecular phylogeny of Soims-laubachia （Brassicaceae） s.l., based on multiple nuclear and plastid DNA sequences,and its biogeographic implications

The Hengduan Mountains region of south-west China is a noted biodiversity,hotspot, but the geographic origins and historical assembly of its rich endemic flora, including the sky-island species ofSolms-laubachia Muschl. （Brassicaceae）, have been little studied. Previous molecular studies on the phylogeny of Solms-laubachia showed it to be paraphyletic, leading to considerable expansion not only of its taxonomic limits, but also its geographic range, with the inclusion of taxa from outside the Hengduan region. However, these studies provided little resolution of interspecific relationships, preventing inferences about historical biogeography within the clade. In the present study, new sequence data from two nuclear genes （LEAFY and G3pdh） and two chloroplast intergenic spacers （petN-psbM and psbM-trnD） were combined with existing markers to increase phylogenetic signals. Phaeonychium villosum （Maxim.） Al-Shehbaz was found to be nested within Solms-laubachia s.l. In general, phylogenetic relationships appear to be a good predictor of geography, with the Hengduan Mountain endemics embedded in a paraphyletic grade of species from the western Himalayas and central Asia, but they also imply morphological homoplasy, lncongruence was detected between the nuclear and chloroplast gene trees, perhaps resulting from incomplete lineage sorting of ancestral polymorphisms. The crown age ofSolms-laubachia s.l. was estimated to be approximately 1.42-3.68 mya, using Bayesian relaxed molecular clock analysis. Historical biogeographic analysis using a parametric dispersalextinction-cladogenesis model inferred central Asia and the western Himalayas as most probable ancestral range of Solms-laubachia s.l., and estimated higher rates of eastward expansion than westward during the diversification of descendant lineages. In summary, our results suggest that Solms-laubachia s.l. originated during the Pliocene in central Asia, and subsequently migrated eastward into the Hengduan Mountains, colonizing sky-island, alpine scre     

Inhibition of invasiveness and expression of epidermal growth factor receptor in human colorectal carcinoma cells induced by retinoic acid

INTRODUCTIONAsaderivativeofvitaminA,RAcaninhibittheproliferati0nofmanymalignantcel1sallde1icitdifferentiationinsometumorce1lsI1-5].RecentstudieshaveshownthatRAmodu1atessynthesisofover4Oproteinsthroughitsnucleicrecept0r[6].Forinstance,RAinducesthesynthesis0ffibronectin(FN)incertaintumorcellsandactivatesthegenecodingfOrB1sllbunitoflaminin(LN)causingitsexpressi0n[7,8].FNandLN'areknownt0bethemostimportantcomponentsofnon-c0llageng1ycoproteinsintheextracellu1armatrixandareclose1yrelatedt…     

Ac／Ds转座系统在水稻转化群体中的转座活性及转座子插入位点旁侧序列的分析

利用本实验室构建的转Ac(Ac TPase)及Ds(Dissociation)的水稻(Oryza sativa L.)转化群体,配置了Ae×Ds的杂交组合354个。检测了转基因植株的T-DNA插入位点右侧旁邻序列,研究了Ac/Ds转座系统在水稻转化群体中的转座活性。结果表明,有些转化植株T-DNA插入位点相同或相距很近,插入位点互不相同的占65.4％。检测到T-DNA可插入到编码蛋白的基因中。在Ac×Ds的F2代中,Ds因子的转座频率为22.7％。对Ac×Ds杂交子代中Ds因子旁侧序列的分析,进一步表明了Ds因子在水稻基因组中的转座活性,除了从原插入位点解离并转座到新的位点之外,还有复制——转座和小完全切离等现象。获得的旁侧序列中,有些序列与GenBank中的数据没有同源性,目前有2个DNA片段在GenBank登录。探讨了构建转座子水稻突变体库进行水稻功能基因组学研究的策略。     

New Species of Meconopsis （Papaveraceae） from Mianning,Southwestern Sichuan,China

Two new species of Meconopsis, M.pulchella and M.heterandra are described and characterized. Differences with similar species are discussed and photos showing the diagnostic features are provided.     

New Species of Meconopsis (Papaveraceae) from Mianning,Southwestern Sichuan

Two new species of Meconopsis, Mpulchella and Mheterandra are described and characterized. Differences with similar species are discussed and photos showing the diagnostic features are provided.     

大鼠早期三级卵泡中的卵母细胞能在联合培养系统中成批适当成熟

我们建立了由离体同步未分化颗粒细胞、动情前期垂体前叶和Δ-4雄烯二酮组成的“联合培养系统”。该联合培养系统可模拟正常生理状态下各相关细胞间的协同作用,更有效地诱发经DES 刺激后所获得的大量大鼠早期三级卵泡中的卵母细胞同步达到适当成熟,从而获得符合正常生理标准的成熟卵球,使卵巢内大量卵母细胞得到充分利用。我们的实验表明,经DES刺激,每侧幼龄大鼠卵巢中平均可取得处在早期三级卵泡中的189枚卵母细胞。在联合培养系统中培养,78%的卵母细胞排出第一极体达到成熟。这些成熟的卵母细胞中88%可正常受精,93%卵裂,经体外培养96h, 有59%的受精卵可发育到桑椹或囊胚。2-细胞期胚胎移植后可发育为健康个体。因此,该联合培养系统为研究大鼠卵母细胞成熟、受精和早胚发育的分子机制提供了非常有效的实验模型。     

Effect of the infectious laryngotracheitis virus (ILTV) glycoprotein G on virus attachment, penetration, growth curve and direct cell-to-cell spread

Glycoprotein G plays many important roles in al-phaherpesvirus except for the varicella-zoster virus (VZV), from which gG is absent[1]. The gG of most alphaherpesviruses is secreted after proteolytic proc-essing, as occurs in the herpes simplex virus 2 (HSV- 2)[2―4], bovine herpesvirus 1 (BHV-1)[5―7], bovine herpesvirus 5 (BHV-5)[8], equine herpesvirus 1 (EHV- 1)[9], equine herpesvirus 3 (EHV-3)[10], equine herpes-virus 4 (EHV-4)[11], feline herpesvirus 1 (FHV-1)[12], infectious la…     

不同钙盐对温州蜜柑抗冻性的影响

用20 mmol·L^-1氯化钙（CaCl₂）、醋酸钙（CaAc₂）和硝酸钙（Ca(NO₃)₂）喷施“国庆1号”温州蜜柑叶片,研究3种钙盐对其叶片抗冻性的影响.结果表明：喷施钙盐降低了叶片半致死温度（LT₅₀）,提高了温州蜜柑的抗冻性,CaCl₂处理的LT₅₀比对照降低了0.54 ℃,而CaAc₂和Ca(NO₃)₂处理分别比对照降低了1.34 ℃和1.35 ℃,表明CaCl₂处理效果较差,CaAc₂和Ca(NO₃)₂处理效果较好.钙盐处理还提高了“国庆1号”温州蜜柑叶片在低温（-9 ℃）胁迫下的SOD、POD活性及可溶性蛋白质、可溶性糖和脯氨酸含量,减少了叶片MDA的积累.