江苏省盐城滩涂野生动物资源调查研究

盐城滩涂野生动物资源丰富.据调查和现有资料统计,共有脊椎动物743 种,其中兽类31种,鸟类394 种,爬行类26 种,两栖类8 种,鱼类284 种,区系成分以古北界种占明显优势.有国家Ⅰ级重点保护野生动物17 种,国家Ⅱ级重点保护野生动物79 种,而且是保护丹顶鹤Grus japonensis、麋鹿Elaphurus davidianus等珍稀物种的关键地区.对盐城滩涂野生动物资源的特点进行了分析.     

二种抗有丝分裂化合物诱发小鼠联会复合体损伤的研究

以抗有丝分裂化合物秋水仙素和对苯二酚处理雄性小鼠,分析了减数分裂前期细胞联会复合体出现的各类损伤。二种化合物在减数分裂前期都诱发各种特殊倾向性的联会复合体损伤(如联会复合体断裂、联会异常等现象)。联会复合体分析,可以作为监测减数分裂过程中源染色体联会异常所引起的染色体异常分离和染色体结构损伤的手段。 Abstract:Two anti-mitotic chemicals(colchicines and hydroquinone)were assayed for their effects on synaptonemal complex(SC)damage in male mice.The tested chemicals significantly induced SC anomalies including SC breakage,asynapsis and non-homologous.It is concluded that SC analysis could be used to pre-screen aneugenes and clastogenes in mammalian germinal cells.     

The herbal compound geniposide rescues formaldehyde-induced apoptosis in N2a neuroblastoma cells

The herbal medicine Tong Luo Jiu Nao(TLJN)contains geniposide(GP)and ginsenoside Rg1 at a molar ratio of 10:1.Rg1 is the major component of another herbal medicine,panax notoginseng saponin(PNS).TLJN has been shown to strengthen brain function in humans,and in animals it improves learning and memory.We have previously shown that TLJN reduces amyloidogenic processing in Alzheimer’s disease(AD)mouse models.Together this suggests TLJN may be a potential treatment for patients with dementia.Because chronic damage of the central nervous system by formaldehyde(FA)has been presented as a risk factor for age-associated cognitive dysfunction,in the present study we investigated the protective effect of both TLJN and GP in neuron-like cells exposed to FA.FA-exposed murine N2a neuroblastoma cells were incubated with TLJN,its main ingredient GP,as well as PNS,to measure cell viability and morphology,the rate of apoptosis and expression of genes encoding Akt,FOXO3,Bcl2 and p53.The CCK-8 assay,cytoskeletal staining and flow cytometry were used to test cell viability,morphology and apoptosis,respectively.Fluorescent quantitative real-time PCR(qRT-PCR)was used to monitor changes in gene expression,and HPLC to determine the rate of FA clearance.Treatment of N2a cells with 0.09 mmol L?1 FA for 24 h significantly reduced cell viability,changed cell morphology and promoted apoptosis.Both TLJN and GP conferred neuroprotection to FA-treated N2a cells,whereas PNS,which had to be used at lower concentrations because of its toxicity,did not.Our data demonstrate that TLJN can rescue neuronal damage caused by FA and that its main ingredient,GP,has a major role in this efficacy.This presents purified GP as a drug or lead compound for the treatment of AD.     

Deoxyribozymes inhibit the expression ofperiod1 genein vitro

Throughout biology, a broad range of biochemical and physiological processes oscillate with approxi-mately 24-h rhythms or circadian rhythms as synchro-nizing with the rhythmic environment (day/night cy-cles, seasons, etc.). The circadian rhythms are under the control of an endogenous oscillator, the circadian clock[1]. Period (per), the first genetically identified circadian mutant[2], is assumed to be a key molecule in the regulation and functioning of the mammalian cir-cadian clock which is…     

红花玉兰等5个玉兰种花粉形态观察及分类学意义

通过对红花玉兰（Magnolia wufengensis L. Y. Ma et L. R. Wang）等5个玉兰种的花粉的光学显微镜和扫描电子显微镜观察拍照,测量光学显微镜下的花粉大小并对其进行统计学分析。结果表明：光学显微镜下,木兰属5个种的花粉均呈现椭圆形或近圆形,各种之间大小、形状方面有较大差异,种内部也存在不同程度的差异;扫描电子显微镜下,各种花粉均为舟形,且具有闭合、长及两端的远极萌发单沟等一致特征,花粉表面纹饰、突起等特征在种内部比较稳定,不同种之间差异明显,可以为木兰属植物种的划分提供比较准确的依据。     

齿孔酸对高尿酸血症黄嘌呤氧化酶活性的影响

采用紫外分光光度法检测齿孔酸在体外对黄嘌呤氧化酶的作用,并进行动力学研究探讨其作用机制;采用酵母联合氧嗪酸钾诱导高尿酸血症小鼠模型,观察齿孔酸对高尿酸血症小鼠血清尿酸水平、血清黄嘌呤氧化酶活性、肝脏黄嘌呤氧化酶活性及血糖血脂的影响。研究发现,齿孔酸体在外能抑制黄嘌呤氧化酶活性,降低高尿酸血症小鼠血清尿酸水平、血清黄嘌呤氧化酶活性、肝脏黄嘌呤氧化酶活性,同时明显降低空腹血糖、总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平,升高高密度脂蛋白胆固醇水平,提高口服糖耐受量。结果表明,齿孔酸是黄嘌呤氧化酶竞争性抑制剂,还能缓解高尿酸血症小鼠糖脂代谢紊乱,对高尿酸血症及痛风的防治具有潜在意义。     

鸟类羽毛色素的合成机理与调控机制

鸟类作为色彩最丰富的陆生脊椎动物,其体表覆盖着颜色多样的羽毛,在伪装、择偶、信号识别等多方面具有重要功能,因此羽毛颜色引起了研究者的极大兴趣。羽毛颜色总体分为由化学物质产生的色素色和由物理结构产生的结构色,其中常见色素有两大类。根据近年来对羽毛色素的研究进展,本文总结了黑色素和类胡萝卜素的类型、合成途径、获取途径以及相关基因,为深入研究羽毛色素合成、代谢的分子调控机制提供科学依据。     

Comparing the frequencies of restriction fragment length polymorphisms for dystrophin gene in Chinese with those from Japanese and Caucasian populations

The restriction fragment length polymorphisms distribution and frequency of dystrophin gene in Chinese were studied by using 14 subclones of the entire 14kb cDNA for the dystrophin as hybridization probes. Allelic fragments were detected in hybridization patterns of PvuⅡ/la, Taq Ⅰ/2b-3, Taq Ⅰ/5b-7, and Xba Ⅰ/10. Among them, the allelic fragments (26kb and 3.8kb) in PvuⅡ/2b-3 pattern and the allelic fragments (10.0kb and 8.4kb) in Taq Ⅰ/5b-7 patterns had never been reported previously. Compared with the data from Caucasians and Japanese, it indicated that there was a significant difference (P<0.01) of the allelic fragment frequency in Taq Ⅰ/2b-3 and Xba Ⅰ/10 patterns between Chinese and Caucasians. The frequencies of allelic fragments A2 (5.6kb) in Taq Ⅰ/8 and A2 (10.Tkb) in EcoR Ⅴ/9 were high in Caucasians, yet had not been detected in Chinese, the differences were also highly significant. But in Chinese and Caucasians, the B1B2 allelic frequencies in Taq Ⅰ/5b-7 are the same. As to the frequency of the allelic fragments A1A2 and B1B2 in Pvu Ⅱ/la, there was no significant difference between Chinese and Japanese.     

Pathogenesis and Associated Diseases of Kaposi＇s Sarcoma-associated Herpesvirus

Kaposi＇s sarcoma-associated herpesvirus （KSHV） is the primary,etiological agent of Kaposi＇s sarcoma, primary effusion lymphoma and muticentric Castleman＇s disease. In common with the other herpesviruses, KSHV exhibits both latent and lyric life cycles, both of which are characterized by distinct gene expression profiles and programs. KSHV encodes proteins which play essential roles in the inhibition of host adaptive and innate immunity, the inhibition of apoptosis, and the regulation of the cell cycle. KSHV also encodes several proteins which have transforming and intrcellular signalling activity.     

紫花苜蓿逆境胁迫诱导相关转录因子MsDREB1基因克隆与分析

利用RT-PCR方法,从紫花苜蓿中扩增得到一个新的转录因子MsDREB1基因cDNA,克隆该cDNA并进行序列分析,结果表明该cDNA包含一个长651bp的开放阅读框,编码一条含216个氨基酸的多肽,分子量约为24．9kDa,等电点为6．11。蛋白质Blast数据显示,该多肽属于EREBP／AP2家族DNA结合蛋白的典型成员。进一步克隆了该基因的基因组DNA,序列分析表明该基因无内含子。Southern blot分析表明,该基因在紫花苜蓿基因组中以2拷贝存在。