鸡胚脑细胞磷脂和能量代谢的模拟微重力生物效应的31P-NMR谱研究

利用31P-NMR方法对回转器旋转的鸡胚脑细胞内磷酸单脂(PME)、磷酸双脂(PDE)及ATP和pH值进行了定量分析, 以研究对脑细胞磷脂代谢及能量代谢的模拟微重力生物效应. 实验结果显示出: 对E13鸡胚旋转24 h引起了ATP和PME及pH值水平的显著性升高. E15鸡胚经24 h旋转对脑细胞磷脂代谢及能量代谢产生类似影响, 但皆未达显著性差异水平. 还利用孔雀石绿方法测量旋转对鸡胚脑细胞 ATPase活性的影响. 从实验结果观察到: E13和E15鸡胚经24 h的旋转引起了ATPase活性的显著下降, 前者比后者更敏感. 停止旋转24 h后, 鸡胚脑细胞所出现的上述变化, 除ATP仍然高于对照外, 其他指标皆可以恢复.     

脉冲电场对人红细胞膜结构影响的冷冻断裂研究

对外加脉冲电场处理的人红血球冷冻断裂和蚀刻的复型观察中发现在强电场作用下,细胞周围有颗粒状和纤维状结构。结合ＳＤＳ电泳分析证明了它们是由于在电场作用下,红血球膜的带３蛋白和膜骨架蛋白（血影蛋白）脱出的结果。在强电场作用下,由于膜蛋白和膜骨架蛋白的脱出造成了对细胞膜的损伤,使细胞膜稳定性降低,细胞易变形和形成伪足。由于膜蛋白的脱出,多余的自由脂进入细胞质内而形成泡状结构。外电场改变了蛋白－蛋白以及蛋     

脉冲电场对鸡胚脑细胞Ca~(2 )和cAMP含量的影响

利用ｆｕｒａ－２／ＡＭ荧光方法和放射免疫方法分别研究了脉冲电场对１１天和１４天胚龄的悬浮鸡胚脑细胞内Ｃａ２＋和环腺苷酸（ｃＡＭＰ）含量的影响,并探讨了在脉冲电场作用下这两种信号物质变化的相关性。实验结果表明：当电激液中［Ｃａ２＋］为１ｍｍｏｌ／Ｌ时,在宽度为１００微秒．强度为０．５,１．０,２．０ｋＶ／ｃｍ的脉冲电场作用下,１１天胚龄的鸡胚脑细胞内钙离子浓度（以［Ｃａ２＋］ｉ表示）与对照相比显著上升,其升高值随着脉冲强度的增加而增加,而细胞内ｃＡＭＰ含量与对照相比显著降低;用ＥＧＴＡ络合电激液中游离Ｃａ２＋以后,虽然细胞内［Ｃａ２＋］ｉ显著上升,细胞内ｃＡＭＰ含量没有显著变化。１４天胚龄的鸡胚脑细胞内［Ｃａ２＋］ｉ在脉冲电场作用下显著上升,而细胞内ｃＡＭＰ含量没有显著变化。脉冲电场对１１天和１４天胚龄的鸡胚脑细胞膜和细胞内Ｃａ２＋库Ｃａ２＋转移系统的开放均有显著作用,此作用具有对脉冲强度的依赖性。为了解物理信号跨细胞膜传导的方式提供了初步的实验根据     

Spontaneous and light-induced photon emission from intact brains of chick embryos

Photon emission (PE) and light-induced photon emission(LPE) of intact brains isolated from chick embryos have been measured by using the single photon counting device. Experimental results showed that the intensi-ty level of photon emission was detected to be higher from intact brain than from the medium in which the brain was immerged during measuring, and the emission intensity was related to the developmental stages, the healthy situation of the measured embryos, and the freshness of isolated brains as well. After white light illumination, a short-life de-layed emission from intact brains was observed, and its relaxation behavior followed a hyperbolic rather than an expo-nential law. According to the hypothesis of biophoton emission originating from a delocalized coherent electromagnetic field and Frohlich's idea of coherent long-range interactions in biological systems, discussions were made on the signifi-cance of photon emission in studying cell communication, biological regulation, living system'     

用电子自旋共振技术对大豆根瘤菌1132-2活性的研究

用电子自旋共振(ESR)方法对液体培养的大豆根瘤菌1132—2的完整细胞在常温下进行了测量,并得到了g=2.0024的自由基信号.该信号的强度随菌的培养时间而改变,培养的菌处在对数生长期的信号最大.     

Anchoring of c-myc on nuclear matrix proteins in process of mouse thymic T lymphocyte proliferation induced by ConA

Isolation and characteriation of functional nudear matrix proteins involved in DNA anchoring and gene expression is one of the major subjects of current nudear matrix research. Southwestern blotting (DNA-protein hybridization) was applied to studying the anchoring of c-myc on the nudear matrix proteins in mouse thymic T lymphocytes. The results showed that c-myc bound to the lamin, p34 and p36 nudear matrix proteins specifically. In the process of mouse thymic PNA T lymphocytes proliferation induced by ConA, the anchoring of c-myc on p34 and p36 nudear matrix proteins changed dynamically.     

超慢生型大豆根瘤菌的生理生化和共生特性的研究

超慢生型大豆根瘤菌(ESG,extra—slow-gfowing soybean rhizobium)是不同于大豆另两类共生体——慢生型大豆根瘤菌(Bradyhizobium,japonicum)和快生型大豆根瘤菌(Sin-orhizobium fredii)的新类群。它们在生长速率和生理学特性等方面均表现出较大的差异。根瘤类菌体的扫描结果表明,ESG的类菌体形态为杆状,与另外两群相近,但发现有“Y”形类菌体。ESG利用碳源范围较窄,抗生素自然耐受性比慢生型低,在柠檬酸盐培养基上不生长;代时超长,已测定的7个菌株代时为23．3-41．9h。细胞成分N,c分析结果表明,ESG在三个类群中N含量最高,C含量最低。温室盆栽试验证明ESG中大部分菌株的固氮酶活性和植株含氮量与生产用菌株相当。ESG菌株可以在绿豆上结瘤并有固氮酶活性。     

血影内Th~（3＋）在脉冲电场作用下外渗的动态研究

利用改进后的Ｔｂ／ＤＰＡ荧光方法对在脉冲电场作用下人血影内Ｔｂ离子外渗的动力学过程进行了系统的研究。对不同场强和脉宽的电场处理的离子外渗量与时间的关系和电穿孔总面积随时间的变化进行了测量。结果表明,离子外渗的主要方式是由膜两侧浓度梯度导致的离子自由扩散。当脉冲宽度或强度两者之一给定的情况下,均存在临界场强和临界脉宽,在临界点以上电场作用下,膜上出现明显的电穿孔。在实验条件下,电穿孔的面积在２００－３００ｍｓ时达最大值,孔的面积和扩展速度与电场参数有关。临界点以下电场处理后,虽仍能测出离子的外渗,膜上可能未出现明显的电穿孔。     

海洋甲藻Pyrocistis elegans电激发光的研究

利用培养的海洋甲藻Ｐｙｒｏｃｉｓｔｉｓｅｌｅｇａｎｓ（Ｐ．ｅｌｅｇａｎｓ）作为模型,研究外电场对细胞发光强度－时间曲线的影响。实验结果表明,细胞在外电场作用下所产生的发光（电激发光）行为与细胞本身有关,电激发光是一种延迟发光,其松驰过程不遵从指数衰减规律而表现出双曲线的规律。从物理学的角度讨论了细胞电激发光的相干性及外电场对生命系统和非生命系统的作用的区别,前者更重要的是信息作用,从而提示了研究外电场对细胞作用以及细胞与环境和细胞与细胞间通讯的新方法。     

Dynamic changes of [Ca~(2 )]_i in cerebellar granule cells exposed to pulsed electric fields

Intracellular free Ca2 concentration ([Ca2 ]i) in embryonic chick cerebellar granule cells loaded with fluo-3/AM and exposed to a single pulsed electric field was investigated using a confocal laser scanning microscope and fluorescent microscope equipped with CCD video imaging system. The results showed that [Ca2 ]i increased immediately and rose to the peak rapidly as the cells exposed to a single pulsed electric field. The amplitude and rate of the increases of [Ca2 ]i depend on the intensity of external electric field. In the presence of Ca2 chelant EGTA or Ca2 channels blocker La3 in the pulsation solutions, the increase of [Ca2 ]i was still observable. It was also observed that [Ca2 ]i of different intracellular areas in the cell elevated simultaneously while the peak of the increase of [Ca2 ]i in the poles of the cell preceded to the peak in its somata and recovered to a plateau within a short time.