长江口中华绒螯蟹放流亲蟹的繁殖力评估

在长江口中华绒螯蟹亲蟹生殖洄游期间, 通过研究中华绒螯蟹放流群体和自然群体繁殖力随壳宽的变化规律, 比较放流群体和自然群体繁殖力的差异, 从而评估人工增殖放流亲蟹的繁殖力。结果显示, 随着壳宽的增大, 中华绒螯蟹放流群体和自然群体的繁殖力都显著增加(P<0.05)。在相同壳宽范围内, 放流群体和自然群体的繁殖力之间没有显著差异(P>0.05)。回归分析显示, 放流群体繁殖力(F)与壳宽(CW)呈幂函数关系: F=3.979CW6.208(R2=0.822);自然群体F与CW呈幂函数关系: F=1.696CW6.636(R2=0.673)。协方差分析显示, 放流群体与自然群体F与CW的两条曲线在显著性为0.05时拟合较好。研究结果表明, 放流群体与自然群体的繁殖力与壳宽之间无显著性差异, 推断放流亲蟹能够适应长江口天然水域环境, 并与自然群体的繁殖力水平相当。     

Establishment of a highly efficient hematopoietic differentiation model from human embryonic stem cells for functional screening

Human embryonic stem cells(hESCs)have been successfully differentiated into hematopoietic progenitor cells with colony formation capacity and further into various kinds of blood cells including erythrocytes,megakaryocytes,neutrophils,nature killer cells and T lymphocytes[1 7].Nevertheless,the differentiation efficiency is extremely low.     

Basal physiological parameters in domesticated tree shrews(Tupaia belangeri chinensis)

Establishing non-human primate models of human diseases is an efficient way to narrow the large gap between basic studies and translational medicine. Multifold advantages such as simplicity of breeding, low cost of feeding and facility of operating make the tree shrew an ideal non-human primate model proxy. Additional features like vulnerability to stress and spontaneous diabetic characteristics also indicate that the tree shrew could be a potential new animal model of human diseases. However, basal physiological indexes of tree shrew, especially those related to human disease, have not been systematically reported. Accordingly, we established important basal physiological indexes of domesticated tree shrews including several factors: (1) body weight, (2) core body temperature and rhythm, (3) diet metabolism, (4) locomotor rhythm, (5) electroencephalogram, (6) glycometabolism and (7) serum and urinary hormone level and urinary cortisol rhythm. We compared the physiological parameters of domesticated tree shrew with that of rats and macaques. Results showed that (a) the core body temperature of the tree shrew was 39.59±0.05 °C, which was higher than that of rats and macaques; (b) Compared with wild tree shrews, with two activity peaks, domesticated tree shrews had only one activity peak from 17:30 to 19:30; (c) Compared with rats, tree shrews had poor carbohydrate metabolism ability; and (d) Urinary cortisol rhythm indicated there were two peaks at 8:00 and 17:00 in domesticated tree shrews, which matched activity peaks in wild tree shrews. These results provided basal physiological indexes for domesticated tree shrews and laid an important foundation for diabetes and stress-related disease models established on tree shrews.     

新辅助化疗对骨肉瘤多药耐药相关蛋白表达的影响

目的:探讨不同新辅助化疗方案对多药耐药相关蛋白在骨肉瘤组织中表达的影响。方法:采用RP-PCR技术以及免疫组化技术检测48例骨肉瘤患者在不同新辅助化疗方案实施前后多药耐药相关蛋白在mRNA以及蛋白水平的表达变化。结果:在同一新辅助化疗方案实施前后以及不同新辅助化疗方案之间,肿瘤组织的多药耐药蛋白的mRNA及蛋白表达均无显著性差异。结论:不同新辅助化疗方案的实施对骨肉瘤多药耐药蛋白表达的影响有限,其多药耐药性可能主要决定于肿瘤本身,检测多药耐药蛋白的表达有利于制定个体化化疗方案。     

利用基因芯片对一高保守基因进行肿瘤相关分析

本文对大规模人类cDNA 测序过程中获得的一条高保守基因进行了初步功能研究,生物信息学研究发现该基因在人类、小鼠、果蝇、拟南芥和裂殖酵母中都有很高的保守性,其他分析预测该基因可能具有肿瘤相关性。RT-PCR分析表明,该基因在成人和胎儿组织中广谱表达。利用基因芯片分析该基因在7例肝癌、5例胰腺癌、2例喉癌和2例肺癌中表达情况,结果证实了该基因的肿瘤相关性,并且提示该基因在不同肿瘤类型中可能处于不同的地位。 Abstract:Preliminary function research of a highly conserved human gene,which was cloned from human fetal cDNA library during large-scale cDNA sequencing,is illustrated in this article.Bioinformatics analysis indicates that this gene is highly conserved in human、mouse、fruit fly、thaliana and fission yeast.Other bioinformatics analysis implies its relevance with tumors.RT-PCR analysis shows its wide-ranging expression patterns.Its expression in 16 cancer cases(including 7 liver cancer cases、5 pancreas cancer cases、2 larynx cancer cases and 2 lung cancer cases) is studied by using gene microarray analysis.The result shows its relevance with tumors and implies it may have different status in different classification of tumors.     

Cloning and characterization of a mouse liver-specific gene mfrep-1, up-regulated in liver regeneration

Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-l/LFIRE-1), a liver-specific protein, is a member of fibrinogen superfamily that exerts various biological activities. However, the function of HFREP-l/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mouse fibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity to HFREP-l/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectively in mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA during regeneration after 70% partial hepatectomy (PHx) in mice, mfrep-1 mRNA increased in the regenerating liver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress the induction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNA continued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced express     

Research of blastocyte-like structure in chicken

Embryonic stem (ES) cells known to be one of the most brilliant stars enlightening the biology science are a major tool to investigate embryonic development and differentiation, functional genomics and applied in medicine territory for cellular therapy. Even though some scientists have already established cultural sys-tems for selection and maintenance of putative avian ES cells, the study on them still drops behind the mammalian and proceeds slowly. The avian ES cells can be used to produce…     

AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis

In nature, plants are subject to changes of tempera-ture. Thus, like other organisms, plants have evolved strategies for preventing damage caused by rapid changes in temperature and for repairing what damage is unavoidable. Heat stress responses have been well documented in a wide range of organisms. In all spe-cies studied, the heat shock (HS) response is charac-terized by a rapid production and a transient accumu-lation of specific families of proteins known as heat shock proteins (Hsps) th…     

Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute

Disuccinimidyl suberate (DSS) intramolecularly cross-linked hemoglobin (Hb) was developed as a novel red blood cell substitute. A multi-angle laser light scattering detector coupled with size exclusion HPLC was applied to determine the molecular weight of the modified Hb. SDS-PAGE was also used as a complement. It was proved that 83.8% of the product was intramolecularly cross-linked Hb with weight-average molecular weights (Mw) of 67.5 kD, 12% was dimeric Hb with Mw of 146.6 kD, and 4.2% was trimeric Hb with Mw of 306.4 kD. The tetramer structure of the cross-linked Hb was stable as shown in size-exclusion chromatography using a mobile phase containing 1 mol/L MgCI2. Analysis by LC-MS demonstrated that the reaction of DSS with Hb mainly took place between the two a subunits within a Hb molecule, resulting in stabilization of the tetramer structure. However, the cross-linking was not site-specific. The P50 of the cross-linked Hb decreased from 21.8 mmHg to 14.3 mmHg, and the Hill coefficient decreased from 2.22 to 1.41. Result of isoelectric focusing showed that the pi of DSS cross-linked Hb was in the range of 4.6-5.2, similar to that of serum albumin. The safety of DSS cross-linked Hb was favored by animal tests on rats and guinea pigs. Exchange transfusion experiment with DSS cross-linked Hb using rats as a model indicated no pressor effect or other significant side effects. The characteristics and properties of DSS cross-linked Hb were also compared with that of diaspirin cross-linked Hb reported in the literature.     

DNA sequence comparative analysis of the 3pter-p26 region of human genome

Most proterminal regions of human chromosomes are GC-rich and gene-rich. Chromosome 3p is an exception. Its proterminal region is GC-poor, and likely to lose heterozy-gosity, thus causing a number of fatal diseases. Except one gap left in the telomeric position, the proterminal region of human chromosome 3p has been completely sequenced. The detailed sequence analysis showed: (i) the GC content of this region was 38.5%, being the lowest among all the human proterminal regions; (ii) this region contained 20 known genes and 22 predicted genes, with an average gene size of 97.5 kb. The previously mapped gene Cntn3 was not found in this region, but instead located in the 74 Mb position of human chromosome 3p; (iii) the interspersed repeats of this region were more active than the average level of the whole human genome, especially (TA)n, the content of which was twice the genome average; (iv) this region had a conserved synteny extending from 104.1 Mb to 112.4 Mb on the mouse chromosome 6, which was 8% larger in size, not in accordance with the whole genome comparison, probably because the 3pter-p26 region was more likely to lose neocleitides and its mouse synteny had more active interspersed repeats.