Field Surveys Suggest Biennial Reproduction Cycle and Competition-triggered Dispersal of the Endangered Chinese Crocodile Lizard

The endangered Chinese crocodile lizard(Shinisaurus crocodilurus) is a habitat specialist living in streams of mountain forests in southern China and northern Vietnam. Conservation efforts are increasing for recovering its wild populations. However, the growth, reproduction, and dispersal ability of crocodile lizards in the wild are largely unknown. We conducted field surveys of the crocodile lizard population in Daguishan National Nature Reserve, one of the largest extant wild populations of cr...     

遗传参数不同估计方法的比较

利用模拟方法比较了5种方差组分估计方法(ANOVA,Henderson-Ⅲ,MLMT,R EMLMT和MIVQUE)对遗传参数的估计效果。结果表明:REMLMT法在各种情况下均能得到较好的参数估值,估计的准确度相对较高;ANOVA方法在小群体和不均衡资料情况下估计效果最差。同时,群体含量和结构能影响各种方法的估计效果, 群体含量较小或资料来源的公畜数太少,将导致遗传参数的估计误差增大,准确性降低。 Abstract:Five methods for estimating components of (Co) variance,including ANOVA,Henderson-III,MLMT,REMLMT and MIVQUE were compared using computer simulation.The results showed that REMLMT could obtain more accurate estimates for all data sets,while the estimates obtained by ANOVA always had greater deviations from the true values,especial for small and well-unbalanced data sets.Also,the effects of estimation were dependent on structure of population size,accuracy of estimates would be decreased when number of sires was too few or data contained small number of animals.     

PCR方法用于奶牛早期胚胎的性别鉴定

根据牛Y染色体上的特异DNA序列合成一对引物, 通过PCR反应对奶牛早期胚胎进行性别鉴定。预测性别与移植胚胎产犊的实际性别相符率为80%。 Abstract:We have obtained the specific DNA segment from the bovine Y-chromosome and used it to design a pair of primer.The sex of embryos at the preimplantation stage have determined by using the polymerase chain reaction.10 months after uterine transfer showed that the rate of accuracy is 80%.     

奶牛和肉牛6个STR基因座遗传多态性研究The Polymorphism Distributions of Six STR Loci in Dairy Cattle and Beef Cattle

利用PCR技术和复合电泳银染技术检测奶牛和肉牛BM2113、BM1862、BMc701、BM2934、TGLA122、BM720等6个STR基因座的多态性分布，并计算该6个基因座的基因频率（Pi）、个体鉴别力（DP）、杂合度（H）、多态信息含量（PIC）、和非父排除概率（PE）。结果显示：6个STR基因座的基因型分布符合Hardy-Weinberg平衡，奶牛中6个STR基因座中BM2113 基因座的DP、H和PIC最高，TGLA122基因座的PE最高。 6个STR基因座的累积个体鉴别力 （CDP）为 0.99997 ，累积非父排除能力（CPE）为 0.98827 。肉牛中6个STR基因座中BM1862 基因座的DP、H、PIC、PE都是最高，6个STR基因座的累积个体鉴别力（CDP）为 0.99999，累积非父排除能力（CPE）为 0.99578 。结果表明，6个STR基因座可用于牛的遗传连锁分析、个体识别和亲子鉴定等研究领域。Abstract：The polymorphism distributions of six STR loci，BM2113，BM1862，BMc701，BM2934，TGLA122，and BM720 were detected in cattle by Polymerase Chain Reaction and multiplex gel electrophoresis followed by silver staining. Gene frequency (Pi)，power of discrimination (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (PE) were calculated．All loci obey Hardy-Weinberg equilibrium. DP, H and PIC of BM2113 locus, and PE of TGLA122 locus are the biggest among six STR loci in Holstein Friesian. Cumulate DP of six STR loci is 0.99997, Cumulate PE of six STR loci is 0.98827. DP, H, PIC and PE of BM1862 loci are the biggest among six STR loci in beef. Cumulate DP of six STR loci is 0.99999, Cumulate PE of six STR loci is 0.99578. These results showed that the six STR loci could be used as linkage analysis, individual identification and paternity test in cattle.     

NIDDM病人空腹血糖遗传度分析

应用数量性状遗传度估计方法(相关回归法), 对50户NIDDM病例核心家庭成员空腹血糖的遗传度进行估计,以探讨遗传和环境因素对血糖这一数量性状的决定作用。结果表明,经年龄和环境因素校正血糖后,用子女对中亲回归估计的空腹血糖遗传度为0.35768±0.2024, 说明遗传因素虽起一定作用,但环境因素的作用更不容忽视,即支持NIDDM为多因子遗传病。空腹血糖的遗传未显示母体效应。 Abstract:The heritabiligy of fasting glucose was estimated on the members of 50 core families by using the method of correlation and regression of quantitative character heritability so as to research the role of genetic and environmental factors in NIDDM.The result showed that the heritability of glucose,adjusted by age and environmental factors,of the offspring on mid-parent was 0.35768±0.2024.This suggested that in NIDDM genetic factors play a role to some extent,but the influence of environmental factors is more significant,i.e. NIDDM being considered as a multifactorial disease.The genetics of fasting glucose did not show the maternal effects.     

一种检测转基因植物细胞中β-葡萄糖苷酸酶(GUS)活性的实用方法

APracticalMethodforDetectionofGUSActivityinTransgenicPlantCellsYinZhongchaoXuYaoYangFanLiBaojian(BiotechnolgyResearchCenter,ZhongshanUniversity,Guangzhou510275)β－葡萄糖苦酸酶（GUS）基因是近年来在植物基因工程研究中应用得最为广泛的报告基因之一’‘’．其产物GUS活性的检测有多种方法,其中最常用的是组织化学染色法和荣光分析法．但前者为定性检测而且如操作不慎很容易产生假阳性,而后者虽可精确定量测定仅需要相应的配套仪器。我们采用的这种方法是首先将植物细胞粗提蛋白在非变性聚丙烯酚胺凝胶中进行…     

The Current Status of Bird Diversity in Yunnan Province

1IntroductionYunnanProvince,whichislocatedinsouthwestofChina(21?8'-29?8'N,97?1'-106?2'E),isgeographicallythemainpartofYunnan-GuizhouPlateau.Itstotalareaisabout383000squarekilometers.About94%ofthetotalareaaremountainousareas.Itslopesdownwardfromnorthwesttosoutheast.ThehighestpointisKageboPeakofMeliSnowMountain,atanelevationof6740m,inthenorthwestofYunnan.Thelowestpointis76.4mabovesealevelinYunjiang(RedRiver)outletofsoutheastofYunnan.YunnanProvinceiswithinthezoneofmonsoonclimateintropics…     

河豚鱼与人类基因组计划

河豚鱼与人类基因组计划周巍杨焕明刘国仰（中国医学科学院基础医学研究所;中国协和医科大学基础医学院,北京１００００５）ＰｕｆｅｒｆｉｓｈａｎｄＨｕｍａｎＧｅｎｏｍｅＰｒｏｊｅｃｔｓＺｈｏｕＷｅｉＹａｎｇＨｕａｎｍｉｎｇＬｉｕＧｕｏｙａｎｇ（Ｉｎｓｔｉｔ...     

试论光(温)敏核不育水稻异质性的遗传基础

试论光（温）敏核不育水稻异质性的遗传基础杨金水（复旦大学遗传学研究所,上海２００４３３）ＧｅｎｅｔｉｃＢａｓｉｓｏｆＨｅｔｅｒｏｇｅｎｅｉｔｙｏｆＰｈｏｔｏｐｅｒｉｏｄＳｅｎｓｉｔｉｖｅｏｒＴｈｅｒｍｏ－ｓｅｎｓｉｔｉｖｅＧｅｎｉｃＭａｌｅＳｔｅｒｉ...     

诱变技术在作物育种中的应用

本文简述了作物诱发育种的发展现状,重点介绍了当今应用最广泛的电离辐射和化学诱变的原理其及在作物育种上的最新研究成果。