褐家鼠精母细胞中联会复合体的研究Ⅳ.常染色体联会复合体的发育和偶线期节的研究

采用表面铺展－SDS处理、硝酸银和磷钨酸(Phosphotungstic acid,PTA)染色电镜技术,研究了褐家鼠精母细胞中常染色体联会复合体（Synaptonemal complex, SC）的发育及偶线期节（Zygotene nodule, ZN）。在褐家鼠精母细胞的细线期,常染色体轴心（Axial cores, ACs ）已形成,同源轴心在空间上靠近,偶线期SCs 开始形成,到粗线期SCs 完全形成,于双线期SCs 开始解体。在双线期除了个别SCs 侧生组分分开外,大多数SCs 发生碎片化（fragmentation）。在偶线期未配对的ACs 和SCs 侧生组分及中央组分上均发现电子密度高的球形或椭圆形的节状结构――偶线期节,ZNs 在同源染色体配对过程中起很重要的作用。 Abstract　Zygotene nodules(ZNs) and development of the brown ratRattus norvegicus caracohave been studied, by surface spresding with SDS treatment, silver and phosphotungatic acid(PTA) staining techniques. The results as follows: Chromosomal axial cores(ACs) formed during leptotene nuclei in spermatocytes of the brown rat, some homologous axial cores were close to each other. The fomation of SCs starts at zygotene, completed at pachytene and disintegrates at diplotene. In diplotene, lateral elements of a few SCs separate and the presence of fragmented SCs takes place. During zygotene on unpaired ACs and lateral elements and central elements of SCs, there are dense spherical or elliptical nodules-zygotene nodules(ZNs). They play an important part in the process of homologue pairing.     

氯化钇和氯化镨引起的人淋巴细胞DNA分子损伤的研究 A Study of DNA Damage in Human Lymphocytes Induced by Yttrium Chloride and Praseodymium Chloride

用单细胞凝胶电泳法检测了稀土化合物氯化钇和氯化镨对人外周血淋巴细胞的DNA损伤效应。结果表明,与对照相比,3种不同浓度的氯化钇和氯化镨均可引起淋巴细胞DNA受损后DNA迁移率的显著升高,受损伤细胞的百分率与对照差异明显,提示氯化钇和氯化镨具有一定的遗传毒性。 Abstract:The effect of DNA damage in human lymphocytes induced by yttrium chloride and praseodymium chloride was detected using SCG assay.The results showed that a highly significant increase in DNA migration in DNA-damaged lymphocytes was induced by three different concentrations of yttrium and praseodymium compared with the control,The percentage of DNA-damaged have genetic toxicity.The relevant points to this study are discussed.     

Scalene node biopsy; an analysis of 42 cases

Scalene node biopsy was done in 42 patients with previously undiagnosed intrathoracic lesions. A definite histologic diagnosis was obtained in 25 patients. Scalene node biopsy is not only a valuable diagnostic procedure for intrathoracic lesions, but many times it may obviate the necessity of exploratory thoracotomy. If a diagnosis of carcinoma of lung is established by scalene node biopsy, the operability of the patient and the type of pulmonary resection should be carefully evaluated.     

盐生植物碱地肤对盐碱胁迫的生理响应特点

以天然盐生植物碱地肤(Kochia sieversiana)为材料,采用人工生态模拟方法对其施加0~480mmol/L的盐胁迫(摩尔比1∶1的NaCl和Na2SO4)和碱胁迫(摩尔比1∶1的NaHCO3和Na2CO3),测定了盐碱胁迫下的相对生长率(RGR)、叶绿素含量及丙二醛含量.结果表明:低浓度的盐胁迫(80mmol/L)对碱地肤的生长具有刺激作用,其在高达480mmol/L的盐胁迫或400mmol/L的碱胁迫下仍能存活并维持一定的生长,具有强抗盐性和强抗碱性;在相同盐浓度下,碱地肤受碱胁迫的伤害强度和对碱胁迫所做出的胁变反应均大于对盐胁迫.可见,碱地肤的抗盐性强于抗碱性,受碱胁迫的伤害大于盐胁迫,维持高含水量可能是碱地肤适应高强度盐碱胁迫的重要特性.