Accumulation of cadmium and its effects on growth, development and hemolymph biochemical compositions in Boettcherisca peregrina larvae （Diptera： Sarcophagidae）

Newly emerged larvae of the fleshfly, Boettcherisca peregrina were exposed to two different CdCl2 concentrations of 100μg/g and 400 mg/g diet fresh weight （DFW）. They were administered in the diets until the end of larval stage. Cd-exposed larvae accumulated significant amounts of Cd and this accumulation increased with the exposure dose and time. The body weights were lightened and lengths of larvae were shortened considerably after Cd exposure, especially at the higher Cd concentration. The total larval duration was also extremely affected due to Cd exposure. The average duration was prolonged significantly by 14 h at the lower Cd concentration, while it was increased by 33.7 h over controls at the higher Cd concentration. A significant decrease in contents of either soluble proteins, total lipids or caloric values in the hemolymph occurred due to Cd exposure throughout the entire tested period but after 120 h of Cd exposure. In contrast, when exposed to Cd with its higher concentration, total sugar contents in the hemolymph were increased strikingly over the whole tested time, except after 96 h of Cd exposure, while they were not apparently altered except after 24 h of Cd exposure at the lower concentration. Thus, it is suggested that Cd exposure shows significant adverse impact on the growth and development, as well as metabolism, in larvae of this fleshfly, depending on its exposure time and dose.     

Possible Involvement of K^＋/Na^＋ in Assessing the Seed Vigor Index

More substances leaked from a higher-vigor seed sample than from a lower-vigor sample. This indicates that, in some cases, electric conductivity does not represent seed vigor level very well, especially for high-vigor seeds. Results from germination, germination index, leachate conductivity, and the ratio of K^＋/Na^＋ from three-seed lots of Chinese cabbage （Brassica pekinensis （Louv.） Rupr） showed that K^＋/Na^＋ correlated well with germination and germination index. The ability of K^＋/Na^＋ to indicate well changes in vigor was further supported by investigation in soybean （Glycine max （L.） Merr.） seeds and another cultivar of Chinese cabbage seeds. Thus, seed leakage of K^＋/Na^＋ can accurately indicate seed vigor, whereas the conductivity test failed to do so. Furthermore, K^＋/Na^＋ showed up bigger quantitative differences in vigor level than did the conductivity test. This findings provide a more sensitive and accurate index for the assessment of seed vigor. The mechanisms of Na^＋ and K^＋ ion transport are also discussed.     

皖南汉族的跖纹研究

本文分析了安徽皖南地区520例(男女各260人)汉族健康人的跖纹样本。追踪了跖纹A、B、C、D、E和P线止区,发现A、B、C、D线多止于1区;E 线主要走向13区;p三叉缺失较多,P远侧射线止区较高,而腓侧射线止区较低。母趾球纹为L>W>A。各趾间区真实花纹依次用为Ⅲ>Ⅱ>Ⅳ。足小鱼际远侧真实花纹较多,其他跖区较少。在性别、民族和人种间进行了比较,并对差异的原因进行了讨论。     

RAPD重建的大豆属植物的亲缘关系

利用８种ＲＡＰＤ引物（ＯＰＨ－２、ＯＰＨ－３、ＯＰＨ－５、ＯＰＨ－１２、ＯＰＨ－１５、ＯＰＨ－１６、ＯＰＨ－１８和ＯＰＨ－２０）对大豆属的２１份植物材料,其中包括Ｇｌｙｃｉｎｅ亚属的１０个种和Ｓｏｊａ亚属的３个种,进行了基因组指纹图谱构建。通过对获得的基因组指纹图谱的量化分析,利用Ｕｎｗｅｉｇｈｔｅｄｐａｉｒｇｒｏｕｐｗｉｔｈｍａｔｈｅｍａｔｉｃａｖｅｒａｇｅ（ＵＰＧＭＡ）对大豆属中的各个种进行了亲缘关系重建。重建的亲缘关系表明：Ｇ．ｔｏｍｅｎｔｅｌｌａ种中存在３种不同的进化类型,其分化距离已大于某些种种间的分化距离,它们可能是被形态遮蔽的３个种。Ｓｏｊａ亚属内３个种的分化关系同前人的研究推断相同,其亲缘关系很近,这一结果支持将这３个种归并为一个种的观点。但是重建的亲缘关系未能显示出大豆属两个亚属的划分格局。     

Nucleotide sequence of coat protein gene for GPV isolate of barley yellow dwarf virus and construction of expression plasmid for plant

GPV is a Chinese serotype isolate of barley yellow dwarf virus (BYDV) that has no reactionwith antiserum of MAV, PAV, SGV, RPV and RMV. The sequence of the coat protein (CP) of GPV isolate of BYDV was identified and its amino acid sequence was deduced. The coding region for the putative GPV CP is 603 bases nucleotides and encodes a Mr 22218 (22 ku) protein. The same as MAV, PAV and RPV, GPV contained a second ORF within the coat protein coding region. This protein of 17024 Mr (17 ku) is thought to correspond to the Virion protein genome linked (Vpg). Sequence comparisons of the CP coding region between the GPV isolate of BYDV and other isolates of BYDV have been done. The nucleotide and ammo acid sequence homology of GPV has a greater identity to the sequence of RPV than those of PAV and MAV. The GPV CP sequence shared 83.7% of nucleotide similarity and 77.5% of deduced amino add similarity, whereas that of the PAV and MAV shared 56.9%. 53.2% and 44.1%. 43.8% respectively. According to BYDV-GPV CP seque