排序方式: 共有54条查询结果,搜索用时 562 毫秒
41.
以一个鸡的小卫星cMS18为探针,对五指山小型猪、长白猪及枫泾猪进行了DNA指纹分析。结果表明,五指山小型猪的相似系数(0.721)显著高于长白猪(0.503)和枫泾猪(0.484),在遗传背景上也与后两者相距甚远。家系分析表明, 鸡的小卫星探针产生的猪的DNA指纹图的遗传符合孟德尔规律。
Abstract:A chicken minisatellite probe cMS18 was used to generate DNA fingerprints of Wuzhisshan microping,Landrace pig and Fengjing pig.It was revealed that similarity coefficient of Wuzhishan microping(0.721)was much higher than both that of Landrace pig(0.503)and of Fengjing pig(0.484).It was shown that Wuzhishan micropig was genetically distant to Landrace pig and Fengjing pig.Analysis of a porcine pedigree using cMS18 probe manifested that the segregation of DNA fingerprint bands was consistent with the Mendelian fashion. 相似文献
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李胜 顾牛范 冯国鄞 刘万清 沈韬 贺林 LI Sheng GU Niu-fan FENG Guo-yin LIU Wang-qing SHEN Tao HE Lin 《遗传》1998,20(6):15-16
在研究5-羟色胺2A受体基因多态性与精神分裂症的关联分析中,调查了20 2例精神分裂症患者及202例正常对照。各相匹配组间比较未发现基因型和等位基因频率的显著性差异。结果提示,在中国人群中5-羟色胺2A受体的静态T102C突变与精神分裂症之间不存在关联。
Abstract:Association study between in the T102C polymorphism serotonin 2A receptor gene and schizoprenia was performed in 202 schizophrenics and 202 normal controls.No significant differences of genotype and allele frequencies between the matched groups were found.Our results,which are different from some other studies,excluded the association between a silent T102C change and schizophrenia in the Chinese population. 相似文献
43.
用Amp-FLP方法分析江浙沪汉族人群DIS80位点多态性 总被引:10,自引:0,他引:10
冯哲 张雁征 杨颖 张工梁 陈仁彪FENG Zhe ZHANG Yan-Zheng YANG Ying ZHANG Gong-Liang CEHN Ren-Biao 《遗传》1996,18(4):15-18
采用PCR结合PAGE电泳及银染技术,即Amp-FLP方法,分析了148名江浙沪汉族人中D1S80位点的多态性分布,观察到20种等位基因,53种基因型。首次在国际上发现重复单元数为39的等位基因。该位点观察杂合性为0.86,个体鉴别力为0.96,多态性信息含量为0.83,基因频率分布符合Hardy-Weinb erg法则。本文结果提示,采用Amp-FLP技术分析D1S80位点多态性,样本分型具有较高的准确性及灵敏度,适用于人类民族演变、法医学个体指认、亲子鉴定、遗传病基因连锁分析等研究领域。 相似文献
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XIE Kabin ZHANG Jianwei XIANG Yong FENG Qi HAN Bin CHU Zhaohui WANG Shiping ZHANG Qifa & XIONG Lizhong . National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research 《中国科学:生命科学英文版》2005,48(5):445-451
Rice has become a model plant for genomic studies of monocot species, because of its relative small ge-nome size (430 Mb), high synteny with other impor-tant crop species such as maize, barley and wheat, the release of draft sequences of both indica[1] and japon-ica[2] genomes, and the near completion of the map-based sequencing of rice genome by the Interna-tional Rice Genome Sequencing Project. Currently, more than 340 Mb of non-overlapping genomic se-quences including completely sequenced… 相似文献
45.
冯明亮 季芸 陆琼 马俊 稽月华 杨颖FENG Ming-liang JI Yun LU Qiong MA Jun JI Yue-hua YANG Ying 《遗传》2002,24(4):403-174
利用多重PCR和四色荧光(5-FAM,JOE,NED和ROX)自动化检测技术调查上海地区汉族人群D3S1358、vWA、FGA、D8S1179、 D21S11、 D18S51、D5S818、D13S317、D7S820等9个STR基因座多态性分布并计算该9个基因座的基因频率(Pi)、个体鉴别力(DP)、无偏倚期望杂合性(H)、多态性信息含量(PIC)和非父排除概率(PE)。结果显示:9个STR基因座的基因型分布符合Hardy-Weinberg平衡,9个STR基因座中FGA基因座的DP值最高为0.9584,D8S1179的H值最高为0.9403,D18S51的PIC值最高为0.8560,D18S51的PE值最高为0.7391,9个STR基因座累积个体鉴别力(CDP)为0.9999996,累积非父排除能力(CPE)为0.99991。9个STR基因座适合作为中国人群的遗传标志,用于人类学、遗传疾病基因连锁分析、法医学亲子鉴定和个体识别等研究领域。
Abstract:By multiplex amplification and four fluorescent technique,the polymorphism distributions of nine STR loci,D3S1358,vWA,FGA,D8S1179,D21S11,D18S51,D5S818,D13S317 and D7S820 were investigated in Shanghai Han population.Gene frequency (Pi),power of discrimination (DP),polymorphism information content (PIC) expected heterozygosity (H) and probability of paternity exclusion (PE) were calculated.All loci meet Hardy-Weinberg equilibrium.DP of FGA locus,H of D8S1179 locus,PIC of D18S51 locus and PE of D18S51 locus are the biggest among nine STR loci.Cumulate DP (CDP) of nine STR loci is 0.9999996,Cumulate PE (CPE) of nine STR loci is 0.99991.Nine STR loci could be used as the genetic markers of Chinese population in the studies of anthropology,linkage analysis of genetic disease genes,individual identification and paternity test in forensic medicine. 相似文献
46.
YONG LI JIAN-FENG LU CONG-JING FENG XIN KE WEN-JUN FU 《Insect Science》2007,14(2):93-100
Venom and ovarian proteins in braconid and ichneumonid wasps play an important role in the successful parasitism of their host, especially for immune suppression immediately after oviposition. In this study, we compared the effect of venom and ovarian proteins collected from the female wasps of Macrocentrus cingulum, a polyembryonic parasitoid of the larvae of Ostriniafunacalis, on the encapsulation capacity of Sephadex A- 25 beads at 4 h and 24 h post-injection both in vivo and in vitro. The results showed that the ovarian proteins significantly interfered with the encapsulation capacity of hemocytes in a dose-dependent manner. Spreading and viability of hemocytes in O. furnacalis was not disrupted by venom and ovarian proteins at various concentrations injected. It seems likely that the ovarian proteins from M. cingulum play a role in suppressing the encapsulation capacity of host hemocytes. 相似文献
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YIN Kang WANG XiuFang MA Huan XIE Ying FENG JingJing YANG QinQing & Lü ZhanJun Hebei Key Lab of Laboratory Animal Department of Genetics Hebei Medical University Shijiazhuang China 《中国科学:生命科学英文版》2010,53(5):606-612
The presence of Alu repeats downregulates the expression of the green fluorescent protein(GFP) gene.We found that SV40PolyA(PolyA,240 bp),in either orientation,eliminated the inhibition of GFP gene expression induced by Alu repeats when it was placed between the GFP gene and the Alu repeats.In this study,4 different segments(each 60 bp) were amplified from antisense PolyA(PolyAas) by PCR,and inserted upstream of Alu14 in pAlu14 plasmid(14 Alu repeats inserted downstream of the GFP gene in vector pEGFP-C1 in... 相似文献
49.
LIANG Hui CHEN Hankui SHEN Yayun FENG Qisheng JIN Wei HUANG Wei & ZENG Yixin . Cancer Center Sun Yat-sen University Guangzhou China . Chinese National Human Genome Center at Shanghai Shanghai China 《中国科学:生命科学英文版》2004,47(5):449-453
Nasopharyngeal carcinoma (NPC) is an endemic cancer with geographic and ethnic distribution within southern China and southeastern Asia, particularly in the delta area of Pearl River in Guangdong Province, where the incidence is up to 10 to 30/100000 per year. Epidemiological studies have demonstrated that the involvement of genetic factors along with Epstein- Barr virus (EBV) infections and other environmental factors are contributing to the pathogenesis of this disease in the area with … 相似文献
50.
Dong-Dong QIN Xiao-Li FENG Xun-Xun CHU Chun-Lu LI Long-Bao LV Yuan-Ye MA Xin-Tian HU 《动物学研究》2013,34(2):146-153
In animal societies, some stressful events can lead to higher levels of physiological stress. Such stressors, like social rank, also predict an increased vulnerability to an array of diseases. However, the physiological relationship between social rank and stress varies between different species, as well as within groups of a single species. For example, dominant individuals are more socially stressed at times, while at other times it is the subordinate ones who experience this stress. Together, these variations make it difficult to assess disease vulnerability as connected to social interactions. In order to learn more about how physiological rank relationships vary between groups of a single species, cortisol measurements from hair samples were used to evaluate the effects of dominance rank on long-term stress levels in despotic and less stringent female rhesus macaque hierarchal groups. In despotic groups, cortisol levels were found not to be correlated with social rank, but a negative correlation was found between social rank and cortisol levels in less stringent hierarchies. Low ranking monkeys in less stringent groups secreted elevated levels of cortisol compared to higher ranking animals. These data suggest that variations in the strictness of the dominance hierarchy are determining factors in rank related stress physiology. The further consideration of nonhuman primate social system diversity and the linear degree of their hierarchies may allow for the development of valid rank-related stress models that will help increase our understanding and guide the development of new therapeutics for diseases related to human socioeconomic status. 相似文献