快速可靠的双链PCR产物直接测序法

利用T7DNA聚合酶在低温下仍具较高活性的特点,在热变性后低温下进行测序反应,使用该方法对多种PCR产物进行序列分析均取得较好的结果．     

巢县人年代位置新证据及其意义

本文银山地点钟乳石和骨石样的铀系年代。根据位于第２层顶部一钙板样３次分析的平均值,巢县人的上限年龄应为３１万年,据此似可将这一人类化石从目前分类的早期智人改划为晚期直立人。讨论了钟乳石铀系年代的可信度。银山下部地层骨化石样的分析结果,可引为二法一致的骨化石铀系年龄不足以保证数据真实的例证。     

从簇毛麦和摄威大麦草幼穗愈伤组织诱导植株再生

禾本科植物的组织培养和植株再生是一个比较困难的研究课题。除甘蔗等少数物种外,禾本科大部分种的幼叶愈伤组织不能或只能偶尔分化再生植株。近年来陆续报道来源于玉米(Zeamays)、Sorghum bicolor和黍属(Panicum)的一些种的胚的愈伤组织长期保存着胚胎发生和植株再生的能力。同时来源于水稻(Oryza sativa)、粗厚山羊草和大麦的杂种(Triticum     

蜗牛酶的制备和对酵母细胞壁的作用

1974年本刊第1期曾将蜗牛酶的制备方法做过简单介绍,几年来相继收到许多单位来信或来访索取详细资料,为此我们把有关蜗牛酶的制备方法及该酶对酵母细胞壁的溶解作用等方面的内容刊登出来,供有关科技人员参考。—编者     

食管癌病人放射治疗时外周淋巴细胞微核率的变化以及和染色体畸变率的关系

在辐射诱发的染色体畸变中,除了倒位和相互易位外,几乎都伴有断片的产生。在间期细胞中,这种染色体断片呈现为大小不等的圆形或椭圆形结构,称为微核。观察微核率的变化,称为微核测定。近年来,由于微核测定具有方法简便、观察迅速、鉴别比较容易等一系列特点,在辐射损伤的诊断、辐射防护药的筛选、辐射的细胞遗传学效应研究等     

小牛胸腺染色质的电镜观察

细胞核里的染色质是调节控制生物体新陈代谢和遗传变异的物质基础。要了解染色质在细胞不同生理周期结构与功能瞬间变化的辩证统一关系,间期核染色质的构型是首先需要搞清楚的。近十多年来,这方面的研究也就相应比较活跃。 1965年,DuPraw根据他本人在蜜蜂胚胎细胞染色质方面的工作,以及Ris、Ris和Chandler、Wolfe及Callan等在不同动、植物上的有关工作,提出“折叠的纤维”模型的设想,企图用此说明高等真核细胞染色质和染色体的基本结构。随着定量电镜检查法的应用,DuPraw和     

草鱼肠炎病病原研究的进展

草鱼肠炎病是草鱼中流行广、危害大的一种鱼病。自1953年以来,为了有效地控制此病,对防治方法及其病原进行了研究。1958年从病鱼中分离到一种肠型点状产气杆菌(Aeromonas punctata f.intestinalis),通过对健康鱼腹腔注射感染,获得了阳性结果,因而被定为草鱼肠炎的致病菌。       

从巢县人年代争议谈钟乳石、骨化石铀系定年——与陈铁梅先生商榷

从巢县人年代争议谈钟乳石、骨化石铀系定年─—与陈铁梅先生商榷沈冠军,房迎三（贵州大学化学系,贵阳５５００２５）（安徽省文物考古研究所,合肥２３００６１）《人类学学报》第１４卷第２期发表了陈铁梅（１９９５）先生的文章（下称陈文）,对不久前也在该刊发表的...     

Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7

A case-control study in 1979 on electrical wiring configurations and childhood leukemia had stimulated interest in the issue that extremely low frequency magnetic fields (ELF MF) may have harmful biologi-cal effects especially on the incidence of human can-cer[1]. Since then, a large number of studies have been conducted to follow up this important result[2]. The majority of these studies indicate a weak association between exposure to 50―60 Hz ELF MF and the in-cidence of cancer; however…     

Vitamin E is essential for seed longevity and for preventing lipid peroxidation during germination

Tocopherols (vitamin E) are lipophilic antioxidants synthesized by all plants and are particularly abundant in seeds. Despite cloning of the complete suite of tocopherol biosynthetic enzymes and successful engineering of the tocopherol content and composition of Arabidopsis thaliana leaves and seeds, the functions of tocopherols in plants have remained elusive. To address this issue, we have isolated and characterized two VITAMIN E loci (VTE1 and VTE2) in Arabidopsis that when mutated result in tocopherol deficiency in all tissues. vte1 disrupts tocopherol cyclase activity and accumulates a redox-active biosynthetic intermediate, whereas vte2 disrupts homogentisate phytyl transferase activity and does not accumulate pathway intermediates. Mutations at either locus cause significantly reduced seed longevity compared with the wild type, indicating a critical role for tocopherols in maintaining viability during quiescence. However, only vte2 mutants exhibited severe seedling growth defects during germination and contained levels of lipid hydroperoxides and hydroxy fatty acids elevated up to 4- and 100-fold, respectively, relative to the wild type. These data demonstrate that a primary function of tocopherols in plants is to limit nonenzymatic lipid oxidation during seed storage, germination, and early seedling development. The vte mutant phenotypes also explain the strong selection for retention of tocopherol biosynthesis during the evolution of seed-bearing plants.