国产姜科植物的染色体计数(2)

<正> 本文继续对国产姜科植物7属,14种和1栽培种作了染色体计数观察,其中9种和1栽培种是染色体计数的新纪录(表1,图1)。通过对大豆蔻Hornstedtia hainanensis的细胞学观察,初步确定大豆蔻属Hornstedtia的染色体基数为12(X=12)。     

应用改进的通用荧光PCR引物进行多重STR分型

通过设计通用荧光PCR引物并结合DNA测序系统建立了小鼠的多重STR分型方案.实验针对小鼠基因组设计了两对不同的通用引物序列,标记了FAM荧光的通用序列和"加尾"的位点特异性引物共同用于小鼠的多重PCR的STR基因分型.本研究优化了通用引物和特异性引物间的比例,优化了多重STR-PCR的反应条件,并最终利用该技术方案实现了五重STR分型.实验验证了该方案在多重STR分型中的可行性.与传统的荧光检测PCR产物方案相比,应用通用方案完成多重PCR反应大大节省了实验时间与经费.     

抗溴化脱氧尿嘧啶核苷(BrdUrd)单克隆抗体免疫细胞化学技术中排除非特异荧光干扰的计算机软件研究

由于非特异荧光信号的存在与干扰,在BrdU_1d/DNA二维分布图上G2M细胞簇总是高于G1细胞簇,并且在BrdUrd强度分布图上BrdUrd阴性细胞峰呈不对称性或出现另一小蜂,这造成计算机数据处理困难.经本软件处理后,可使细胞周期分相的“盒分析”(Box—Analysis)更容易、更正确.与利用电子线路补偿处理的方式相比,软件系统不需任何人为调节,自动修正补尝.此软件还可分别显示在各种不同条件下G1、G2M和S相细胞分布的变化.     

冷冻前后的低渗应激对小鼠孵化囊胚存活率的影响

The objective of this study to evaluate the effect of hypotonic stress on developmental potential of hatched blastocysts perivitrification. Hatched mouse blastocysts were vitrified in liquid nitrogen after equilibration in 10% or 20% GL for 5 min and in GFS40 for 30 sec respectively, the survival rates were 93%-97% after the frozen-thawed embryos were cultured in vitro for 24 h. There were no statistical difference between the frozen and the fresh group (P > 0.05). In order to evaluate effects of hypotonic stress on developmental abilities, fresh hatched mouse blastocysts were respectively exposed to 1.00 x, 0.50 x, 0.30 x, 0.25 x and 0.20 x PBS for 30 min, then cultured in mKRB for 24 h, the survival rates were 98%, 99%, 92%, 92% and 50% respectively. The rate in 0.20 X PBS group was significantly lower than in other groups (P < 0.01). When frozen-thawed embryos were directly treated with different osmotic solutions, the survival rates were 88%, 72%, 58%, 11% and 0 respectively in 1.00 x, 0.50 x, 0.30 x, 0.25 x and 0.20 x PBS group. The rate in 1.00 x PBS group was significantly higher than in other groups (P < 0.05). However, when frozen-thawed embryos were first cultured in vitro for 12 h, then exposed to 1.00 x, 0.50 x, 0.30 x, 0.25 x and 0.2 x PBS, the survival rates were 98%, 94%, 82%, 58% and 26% respectively. There was no statistical difference between 1.00 x and 0.50 x PBS group (P > 0.05). Although the rate in 0.30 x, 0.25 x and 0.20 x PBS group was significantly lower than in 1.00 x group(P < 0.01), it was significantly higher than in the same treatment group without in vitro culture(P < 0.05).     

微生物发酵棕榈粕研究进展

目前，棕榈粕因其低廉的价格和丰富的产量成为热门的动物饲料原料，但其高含量的抗营养因子纤维素阻碍了其在动物饲料中的高效应用。微生物发酵处理可以有效地降低棕榈粕中抗营养因子含量，提高其营养价值，并提升其品质。文章对棕榈粕的化学成分及微生物发酵棕榈粕、发酵棕榈粕在动物养殖中的应用研究进展进行综述，旨在为微生物发酵棕榈粕饲料的技术应用提供参考。     

贵州耳蕨属新资料

贵州耳蕨属新资料王筱英王培善（贵州科学院，贵阳５５０００１）ＮＥＷＭＡＴＥＲＩＡＬＳＦＯＲＰＯＬＹＳＴＩＣＨＵＭＦＲＯＭＧＵＩＺＨＯＵＷａｎｇＸｉａｏｙｉｎｇ，ＷａｎｇＰｅｉｓｈａｎ（ＧｕｉｚｈｏｕＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓ，Ｇｕｉｙａｎｇ５...     

Charcot-Marie-Tooth病与遗传性男子女性型乳房的自由组合1)

本文报道Charcot-Marie-Tooth病伴有/不伴有遗传性男子女性型乳房患者的家系，并对这两种种病的诊断、遗传方式和组合问题进行了研究，证实系两个单基因遗传的致病基因在一个家系中发生了自由组合。