Metabolic action of cortisone; D-amino acid oxidase and apparent creatinine formation

Data are presented demonstrating that the d-amino acid oxidase of rat liver is lowered on adrenalectomy and restored to normal by treatment with cortisone. This reaction is responsible for data obtained which indicated that creatine and creatinine formation were under the control of cortisone. The α-keto-γ-methiolbutyric acid formed from the oxidation of methione reacts in the Jaffe reaction as “creatinine.” During the course of incubation of the liver enzyme system a portion of the α-keto-γ-methiolbutyric acid appears in a bound form and is measured by way of the Jaffe reaction as “creatine.” Measurements of creatine formation by a method not subject to these errors show that creatine formation in the rat liver enzyme is very small although creatine formation in guinea pig liver can readily be demonstrated.     

选择性标记基因和V-erbB基因对哺乳动物细胞的转移及其转录产物的检测

近十几年来,应用DNA转移的方法将克隆基因或细胞总DNA导入离体培养的哺乳类细胞,已成为研究真核细胞基因调控的一个重要方法。一般要获得克隆基因稳定表达的细胞系,需要用选择性标记的基因。对于具有TK~-、aprt~-或hprt~-缺陷的细胞,通常可以用相应的互补基因作为选择基因。对于不具有特殊遗传缺陷的细胞,可以用显性转移基因Xgpt,neo或dhfr基因作为选择基因,加     

贵州省副省长访问古脊椎动物与古人类所

２００１年３月１４日 ,贵州省副省长龙超云一行在中国科学院办公厅副主任蒋崇德和资源环境科学技术局副局长范蔚茗陪同下抵达中国科学院古脊椎动物与古人类研究所 ,与所领导及贵州海相三叠系课题组成员进行座谈。朱敏所长首先向客人介绍了所的历史沿革及现在研究所的基本情况 ,着重介绍了围绕知识创新工程所做的工作及取得的成果。贵州省古生物资源非常丰富 ,化石门类多样 ,但省领导却感到摸不清这个家底 ,龙省长此行重要目的之一就是向中国科学院的专家了解贵州省的古生物资源在我国以及世界上所处的位置。龙省长对古生物工作极为重视 ,她…