紫锥菊不定根悬浮共培养中咖啡酸衍生物积累研究

为筛选出紫锥菊不定根共培养最佳组合及其接种量,以紫松果菊(Echinacea purpurea,Epu)、白色紫锥菊(Echinacea pallida,Epa)和狭叶紫锥菊(Echinacea angustifolia,Ean)3种药用紫锥菊不定根为材料,在500 m L三角瓶中加入200 m L的0.5 MS+1.0 mg/L IBA培养基进行了悬浮培养,调查了接种量5 g/L、7 g/L和10 g/L及不定根组合方式对共培养紫锥菊不定根生物量、次生代谢产物积累及抗氧化活性的影响。结果表明,接种量为7 g/L时的Epa+Epu共培养不定根干重(6.75 g/L)最高,此组合中不仅多酚(40.31 mg/g DW)和黄酮(34.29mg/g DW)含量高,其产量(多酚272.09 mg/L,黄酮231.46 mg/L)也最高。紫锥菊不定根共培养组合中,只有Epa+Epu不定根中6种咖啡酸衍生物都具有,而且此共培养不定根还刺激产生Epa和Epu单一培养时未检测到的活性物质咖啡酸,其中接种量为7 g/L时的共培养Epa+Epu不定根中总咖啡酸衍生物产量最高,达136.22mg/L,是Epu单独培养时的1.35倍,Epa单独培养时的1.73倍。还原能力和DPPH自由基清除率也是接种量为7 g/L时的Epa+Epu不定根组合较高。此结果为大规模生产具有抗肿瘤抗氧化药用价值的紫锥菊医学药材提供技术参数。     

PCR介导的cDNA文库矩阵排列筛选方法

描述了一种快速、有效的ｃＤＮＡ文库筛选策略。其主要过程是将ｃＤＮＡ文库重组子进行矩阵排列,进而用特异引物进行ＰＣＲ逐级筛选以分离目的基因．     

Construction of serial deletants and chimeras of multi-kringle containing molecules and primary analysis of their functions

In comparison of amino acid sequences of 4 kringles of both macrophage stimulating protein (MSP) and hepatocyte growth factor (HGF), consensus motif sequence was determined. According to this consensus sequence, a pair of universal primers were designed. In combination with specific upstream or downstream primer of MSP or HGF respectively, serial fragments containing variant number of kringle (from 1 to 4) can be obtained by once PCR. By ligating the C terminal and N terminal fragments with different combination, serial deletants and chimeras of MSP and HGF were constructed. Sequence analysis showed that the degeneracy for universal primers and the sequences of those constructed deletants and chimeras are desired. Biological assay of these deletants revealed that wild type MSP can inhibit the growth of some tumor cell lines and that kringle 1 of MSP is essential for function as that of HGF.     

Gene therapy for rat renal anemia with implantation of erythropoietin-transgenic myoblasts

To investigate whether an erythropoietin (EPO) gene-based therapy could serve as an alternative to the repeated injection of rhEPO in treatment to renal anemia, the genetically modified myoblasts of rats, named Myo/ EPO, were implanted through intramuscular injection to model rats with renal anemia. The hemoglobin (Hb) and hematocrit (HCT) of the rats increased from (92. 5±3.0) g/L and 0.29 ±0.04 to the peak values of (103.8 ±5.0) g/L and 0. 32 ±0. 04 respectively 14 d after implantation, and sustained the pre-implantation level for 90 d. Otherwise, the control rats implanted with Myo/X, which carried the parent retroviral vector, gradually became severe in anemia. The PCR detection for hEPO cDNA in the rat muscle adjacent to injection sites indicated that the Myo/EPO cells survived for a long period in the muscle of rats. The results primarily demonstrate that myoblast gene transfer of EPO is effective for the treatment of rat renal anemia.     

遗传工程植物的病毒抗性

经典的常规抗病育种法虽有其成功的好例子,但其成功率是有限的,因为这些抗性基因仅存在于互交不孕型类型中,可能与不需要的性状连锁着,或可能是多基因的和难于转移的。 分子遗传学和植株转化技术使得利用新途径来提高植物的抗性成为可能,因为它们可克服上述限制。迄今为止,有用的细菌基因和病毒基因已成功地转移入许多植物中。     

枳多芽苗的诱导

枳幼苗茎段的腋芽在含BA(0.5毫克/升)和NAA(2毫克/升)的MS培养基上能诱导出大量丛生芽,丛生芽苗茎段的腋芽在含BA(0.5-2毫克/升)的MS继代培养基上又可增殖,将无根幼苗分别转至含IAA、IBA和NAA(均为1毫克/升)的MS培养基上则生根形成完整植株。     

大河猪几个数量性状遗传参数的初步估计

大河猪是云南省东北部地区具有一定代表 性的地方猪种,一般可分为八卦头、二八卦头、 红毛与黑毛等类型。猪的繁殖和生长发育情况 是重要的经济性状,测定这些性状的遗传参数, 对制定选种方法、设计选择指数、预测选育进 展、提高选种效果,都有一定的现实意义。近几 年国内发表了大白猪和新淮猪遗传参数的研究 报告^[1-2],对推动我国猪育种工作起了很好的作 用。遗传参数虽然具有通性的一面,但也随着 品种、畜群、饲养条件的不同而有差异。我们对 富源县大河种猪场的大河猪进行了繁殖性状和 生长发育性状的遗传力和遗传相关的统计,以 期对大河猪的选育提高工作提供参考。     

大河猪若干性状近交效应分析

近亲交配是猪育种工作中经常采用的一种 繁殖手段,它有分化种群,纯合种群的作用。合 理而有目的地采用近交,可使猪群基因纯合,有 利性状的固定,从而加速品系的建立,促进品种 形成以及提高杂交优势率。但对猪的近交效 果,历来看法不一〔1,2,470     

大亚基rRNA基因D1/D2区序列相同或相似的子囊菌酵母ITS序列比较和核型分析

下面每个组内的酵母菌:I)Candida aaseri和Candida butyri;Ⅱ)Candida boleticola, Candida laureliae和Candida ralunensis;Ⅲ) Candida zeylanoides和Candida krissii以及Ⅳ) Pichia farinosa和Candida cacaoi因具有相同或只有一个碱基差异的大亚基(26S)rRNA基因D1/D2区序列,而被认为属于同一个种。本研究对其ITS序列和电泳核型进行了比较分析。结果表明前3个组内的种具有完全相同的ITS序列,第Ⅳ组的两个种只有1个碱基差异, 但是各组内的核型并不完全一致。第Ⅳ组内的两个种具有完全相同的核型,证实他们属于同一个种。第Ⅱ组内的C.laureliae和C.ralunensis也具有完全相同的核型,可以肯定二者也属于同一个种,该组内的C.boleticola的核型与前二者不完全一样,但染色体分子量范围相似,也可能与前二者属于同一个种。第Ⅰ和Ⅲ组内各种的核型具有明显差异,对组内种间的同物异名关系未提供支持。