The finding of the genus Clonostachys （Hyphomycetes） in China

Clonostachys phyllophila and Clonostachys rogersoniana were isolated from rhizosphere soil of Allium tuberosum in Chaoyang, Liaoning Province. It is the first report of the genus in China. The species was described morphologically and illustrated in this paper, the culture was stored in the Culture Collection of Liaoning Center of Culture Collection （LCCC）.     

利手、优势足及扣手的遗传方式初探

采用Slater区分单基因和多基因遗传的计算模式及Smith无偏分析方法对21个家系资料的分析表明：利手、优势足、扣手特征均为常染色体单基因显性遗传,R型为显性性状。虽然环境因素对这类特征的表现也有一定的影响,但遗传因素仍起主要作用。 Abstract:The data of 21 families were analyzed by the method of Slater's calculating model to differentiate between single-gene and multi-gene heredity and by the method of non-deviation analysis.The results showed that the hereditary mode of handedness or preferential foot or hand-clasping is the dominant heredity of single gene of autosome,and the right type of all of them is the dominant character.In a way,although environmental factors affected the phenotypes of these characters,hereditary factors were also the decisive ones.     

Novel Cellulase Profile of Trichoderma reesei Strains Constructed by cbh1 Gene Replacement with eg3 Gene Expression Cassette

The filamentous fungus Trichoderma reesei is consi-dered to be the most efficient cellulase producer, and hasa long history in the production of hydrolytic enzymes,which was widely used in the food and feed industriesand recently also used in the textile,…     

披碱草X球茎大麦属间杂种的减数分裂研究

对于ＥｌｙｍｕｓｔｓｕｋｕｓｈｉｅｎｓｉｓＨｏｎｄａ（２ｎ＝６ｘ＝４２,ＳＳＨＨＹＹ）和ＨｏｒｄｅｕｍｂｕｌｂｏｓｕｍＬ．（２ｎ＝４ｘ＝２８,ＩＩＩＩ）属间杂种的体细胞有丝分裂和花粉母细胞（ＰＣＭ）减数分裂进行了分析和研究。结果表明：（１）该属间杂种的根尖体细胞染色体数目稳定（２ｎ＝５ｘ＝３５）,但其ＰＭＣ的染色体数日却有较大的变异（２ｎ＝２２～２ｎ＝４ｎ）,即在该属间杂种的一些ＰＭＣ中产生了部分染色体嵉亩Щ蛑馗矗唬ǎ玻└檬艏湓又郑玻睿剑担剑常担校停玫娜旧迮涠允艿停砻鳎牛簦螅酰耄酰螅瑁椋澹睿螅椋笥耄龋猓酰欤猓铮螅酰淼娜旧遄橹涞牟糠滞葱院艿停唬ǎ常龋猓酰欤猓铮螅酰淼耐慈旧遄椋á颍┑呐涠孕形谠又种忻飨允艿揭种疲唬ǎ矗┰谀承┤旧灞欢Я说模校停弥校淙旧遄榕涠允飨栽黾樱饪赡苡胩囟ㄈ旧宓亩в泄兀欢У哪程跞旧骞憧赡苄乓种茘染色体配对的因子Ｅｌｙｍｕｓｔｓｕｋｕｓｈｉｅｎｓｉｓ,Ｈｏｒｄｅｕｍ,属间杂种,减数分裂,染色体配对控制     

鹅观草属三个种的形态变异与核型的研究

本文通过对鹅观草属的三个种:鹅观草(Roegneria kamoji Ohwi)、纤毛鹅观草(R. ciliars (Trin.) Nevski)和竖立鹅观草(R. japonensis(Honda)Keng)的形态学比较和核型分析研究了该三个种的系统关系。R. kamoji与R. ciliaris、R. japonensis形态上的差异明显,而后二个种差异甚小,难以进行划分。R. kamoji的核型公式为:2n=6x=42=30m+8sm+4SAT;R. ciliaris为:2n=4x=28=18m+6sm+4SAT;R. japonensis为:2n=4x=28=18m+6sm+4SAT。t-测验的结果表明R. ciliaris与R. japonensis的核型无显著差异。因此笔者认为R. ciliaris和R. japonensis应合并为一种系内的分类群。     

原位杂交技术在稻属研究中的应用

在文献综述的基础上,对原位杂交技术在稻属的应用进行了介绍,主要包括3个方面:(1)特定 DNA序列的定位;(2)构建染色体的物理图谱及其与遗传图谱之间的关系;(3)稻属基因组间关系的研究。     

小鼠胚胎核移植实验的初步报告

Abstract:Oocytes recovered from Fallopian tubes of female mice of Kun-Ming-Bai strain were enucleated by microsurgery and a single blastomere aspirated from 2-cell stage embryo derived from C57 was injected into the perivitelline space of earh enucleated oocyte.Then those coupled cells were exposed to PEG solution and cultured in an atmosphere of 5% Co2 at 37℃ for four hours.After that,couples which had been fused to one cell were transferred to the oviducts of pseudopregnant foster mothers.Six offsprings were obtained from this transplantation. Key words:Mouse,Embryo,Nuclear transplantation     

转基因栽培稻基因漂移是否会带来环境生物安全影响?

转基因通过基因漂移可以渐渗到作物的野生近缘种,由此而导致的环境风险是全球广泛关注的生物安全问题.有3个关键因素可以决定环境风险的程度:特定空间距离的转基因漂移频率,转基因在野生近缘种中的表达水平,以及转基因为野生近缘种群体带来的适合度效应.本文将根据现有研究结果,从上述3方面对转基因漂移到非转基因栽培稻、杂草稻和野生稻造成的潜在环境影响进行回顾.栽培稻品种之间的基因漂移频率很低,可以通过空间隔离或其他方法使其降低到可忽略的水平.在共同分布的环境中,栽培稻基因(包括转基因)向杂草稻和野生稻的漂移不可避免.尽管抗虫转基因(Bt或Bt/CpTI)在栽培稻和野生近缘种杂交后代中可以正常表达,但由于在低虫压环境中,抗虫转基因不会明显改变野生近缘种的适合度,抗虫转基因漂移所造成的环境影响十分有限.因此对基因漂移而言,抗虫转基因栽培稻的商品化种植应该比较安全.然而,抗除草剂转基因渐渗到杂草稻或野生稻会改变群体的适合度,可能会引起不可预测的环境后果.     

IL-4-independent inhibition of IL-12 responsiveness during Leishmania amazonensis infection

Leishmania amazonensis induces a nonhealing infection in C3H mice, whereas infection with Leishmania major is self-healing. We found that C3H mice infected with L. amazonensis exhibited decreased IL-12 production, which could account for the susceptibility to this organism. However, exogenous IL-12 administration failed to induce a healing immune response. The failure of L. amazonensis-infected C3H mice to respond to IL-12 was associated with a specific defect in IL-12 receptor beta2 (IL-12Rbeta2) mRNA expression by CD4+ T cells. Furthermore, decreased IL-12Rbeta2 mRNA expression correlated with a decrease in the IL-12-signaling capacity of the lymph node (LN) cells. IL-4 did not contribute to susceptibility or down-regulation of the IL-12Rbeta2 subunit, because IL-4-/- mice remained susceptible to L. amazonensis infection, even after IL-12 administration, and CD4+ cells from infected IL-4-/- mice also had reduced expression of IL-12Rbeta2 mRNA. These results demonstrate that regulation of the IL-12 receptor, independent of IL-4, is a point of control for the immune response to leishmaniasis. In contrast to experimental L. major infections, where host genetics control susceptibility, these studies demonstrate that the lack of IL-12 responsiveness may be dictated by the pathogen, rather than the host.