Identification of up-regulated genes in human uterine leiomyoma by sup-pression subtractive hybridization

In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88 genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes were firstly associated with UL. Three genes with notable difference were selected for Northern confirmation. Our results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showed up-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obvious expression in prostate, testis, liver, heart and skeletal muscle.     

两种微藻改善虾池环境增强凡纳对虾抗病力的研究

人工引入波吉卵囊藻和微绿球藻于凡纳对虾养殖环境中,检测与凡纳对虾抗病力有关因子变化和测定主要水质因子,研究微藻生态调控对凡纳对虾抗病力的影响。结果表明,引入波吉卵囊藻和微绿球藻能改善养殖水体的水质,凡纳对虾的血细胞数目,血清蛋白的含量以及酚氧化酶、超氧化物歧化酶,溶菌酶,抗菌酶的活性都较对照组显提高。因此,微藻生态调控是防止对虾疾病的重要技术措施。