树舌灵芝中分离得到非还原性二糖—海藻糖

Trehalose, a non-reducing disaccharide, was separated for the first time from Ganoderma applanatum fruit body. The structure of trehalose was determined by electrospray ionization mass spectrometric and NMR data. The content of trehalose was determined by improved anthrone-sulphuric acid colorimetric method and it was 0.48% of dried weight of G. applanatum fruit body. Mannitol was simultaneously obtained during separation of trehalose from G. applanatum.     

从噬菌体多肽文库中筛选α—葡萄糖苷酶的抑制剂

以黑曲霉葡糖淀粉酶为靶分子,筛选噬菌体展示的多肽文库,经过3轮生物淘选法筛选,得到3个特异性结合的噬菌体克隆,分别命名为GB-1、GB2和GB-3。其插入多肽中都含有一对二硫键,破坏二硫键将大大降低噬菌体与葡糖淀粉酶的结合。化学合成的环状多肽KCHFEECLAY,其序列对应于结合力最强的一个克隆GB-1的N端的10个氨基酸残基,该多肽可以竞争性地抑制黑曲霉葡糖淀粉酶（Ki=0.2mmol/L)以及大鼠小肠的α-葡萄糖苷酶（Ki=1.4mmol/L)。     

短乳杆菌DM9218肌苷水解酶基因的异源表达及活性检测

目的探讨短乳杆菌DM9218肌苷水解酶基因A0008的异源表达及其对肌苷的分解活性检测。方法克隆来源于短乳杆菌DM9218基因组的肌苷水解酶基因A0008,构建原核表达载体,转入大肠埃希菌BL21诱导重组蛋白表达并纯化,进行体外酶活检测。结果成功构建了肌苷水解酶A0008-pET28a原核表达载体,表达并纯化出重组蛋白,酶活结果显示该重组蛋白具有水解肌苷的能力。结论短乳杆菌DM9218基因A0008可能编码肌苷水解酶并参与DM9218对肌苷的分解。     

应用全外显子组测序筛查一高原肺水肿家系易感基因

高原肺水肿(high-altitude pulmonary edema, HAPE)是一种高原特发性疾病,其发病与遗传因素有一定关联。本研究对一个HAPE相关的家系展开遗传学调查,然后利用外显子组测序筛查了包括先证者在内的6名HAPE病史成员以及先证者的母亲共7个成员的遗传变异,结果发现18个HAPE相关的潜在遗传变异(9个SNVs和9个Indels)。利用SIFT,PolyPhen-2和PROVEAN等3种软件对这些遗传变异进行蛋白功能危害性分析,结果发现定位于CFHR4基因的SNV(p.L85F)以及定位于OXER1基因的SNV(p.R176C)具有高危害性,且OXER1的功能与HAPE低氧诱导通路存在高度关联,它们可作为该家系中HAPE相关的候选病理性变异。此外,还有部分SNVs(NMB p.S150P、APOB p.I4194T和EIF4ENIF1 p.Q763P)以及Indels(KCNJ12 p.EE333-334E、ANKRD31 p.LMN251-253LN和OR2A14 p.HFFC175-178HFC),其遗传变异同样具有一定危害,可作为潜在的HAPE相关遗传变异。本研究首次通过外显子组测序直接筛选与一中国HAPE家系相关的遗传变异,为后续揭示HAPE发病机制提供了新线索。     

鼻息肉中HIF-1α、VEGF 和miR-200a 表达及其与复发相关性研究

目的:分析不同病理分型鼻息肉中HIF-1α、VEGF和miR-200a表达及其与复发相关性研究。方法:选取鼻息肉患者42例,在随访期间有15例鼻息肉复发。采用免疫组化SABC法检测鼻息肉及下鼻甲组织中HIF-1α、VEGF表达水平,采用q RT-PCR技术检测鼻息肉及下鼻甲粘膜组织中miRNA-200a表达量。对比分析不同病理分型HIF-1α、VEGF、miRNA-200a表达差异并分析鼻息肉复发的因素。结果:鼻息肉中miR-200a表达量明显低于下鼻甲粘膜组织(P0.05)。鼻息肉中HIF-1α、VEGF表达量明显高于于下鼻甲粘膜组织(P0.05)。鼻息肉病组织中miR-200a表达量明显低于单发鼻息肉、多发鼻息肉(P0.05);鼻息肉病标本中HIF-1α、VEGF表达量明显高于单发鼻息肉、多发鼻息肉组织(P0.05)。鼻息肉复发与鼻息肉的病理分型、HIF-1α、VEGF表达密切相关(P0.05)。结论:鼻息肉增生和局部血管的生成密切相关,HIF-1α可能是同构调节miR-200a表达,来控制VEGF及血管生成的。     

库车种子植物区系垂直分布格局分析北大核心CSCD

Kuche is located in the middle of the Tianshan Mountains in Xinjiang,the northern margin of the Tarim Basin. In terms of geographical division, the vegetation in this region belongs to the warm temperate shrub and subshrub desert area of Xinjiang. Based on field studies and relevant literature, the vertical distribution characteristics of the flora in Kuche were systematically analyzed. The results showed that there were approximately 960 species of seed plants belonging to 78 families and 376 genera in Kuche. Among these, there were nine species of Gymnosperm belonging to three families and three genera. There were 951 species of Angiosperm belonging to 75 families and 373 genara. The total number of families indicated that the highest peak at the elevation with 1 600-1 800 m. The total number of genera indicated that the highest peak at the elevation with 1 800-1 900 m. The total number of species showed that the highest peak at the elevation with 1 900-2 000 m, there were 59 families, 230 genera, 478 species. From the altitude with 2 200-2 300 m, the number of seed plants family, genus, species showed a decreasing trend with increasing altitude, and the distribution curve showed an unimodal peak distribution structure. North temperate elements were dominant in family and genera floristic composition. In vertical gradients, a peak of temperate distribution type appeared in 1 800-1 900 m(including 20 families), from the altitude gradient with 2 800-2 900 m showed a decreasing trend, to 3 600-3 700 m was the lowest(including six families); The temperate distribution peak of the genus appeared in 1 900-2 000 m, and then decreased with the increase of altitude. This study is very important for the characteristics of the altitude gradients on the flora of seed plants of Kuche Mountain in the area of plant germplasm resources and ecosystem diversity, and plant resources of domestication, cultivation and utilization. [ABSTRACT FROM AUTHOR]     

中国小菇属四新记录

报道了中国小菇属4个新记录种,即香小菇Mycena adonis、黄小菇Mycena luteopallens、绿缘小菇Mycena viridimarginata和基盘小菇Mycena stylobates,并提供了详尽的形态描述和线条图。研究标本存放在鲁东大学菌物标本馆（HMLD）和吉林农业大学菌物标本馆（HMJAU）。     

采自长白山的中国鳞伞属新记录

报道了采自吉林省长白山地区的中国鳞伞属 Pholiota新记录4种,即毕格劳鳞伞Pholiota bigelowii、密生鳞伞Pholiota condensa、群生鳞伞Pholiota gregariiformis和酒红褐鳞伞Pholiota vinaceobrunnea,并提供详尽的形态描述和线条图。研究标本存放在吉林农业大学菌物标本馆（HMJAU）。     

中国小香菇属二新种

报道来自中国东北的小香菇属2个新种,即半开小香菇Lentinellus dimidiatus Yu Liu & Tolgor Bau和吉林小香菇Lentinellus jilinensis Yu Liu & Tolgor Bau,文中提供特征描述及线条图,并通过构建系统发育树讨论该两个新种在小香菇属中的系统位置。     

BR—D96N光学薄膜的全息记录特性

BR D96N是具有显著光致变色特性的细菌视紫红质 (BR)经过基因定点突变的产物 ,与野生BR相比 ,它的M态寿命延长到了 5min ,因而表现出明显的饱和吸收特性和较低的饱和吸收光强 (0 .4mW /cm2 )。BR D96N用于全息记录时形成的是动态光栅 ,其特征量为光强而非曝光能量。实验表明 ,当再现光较弱时 ,衍射效率最大的记录光强基本对应于样品的饱和吸收光强。当记录光强一定时 ,再现光对所记录光栅具有擦除作用 ,再现光较弱时具有较高衍射效率 (1.8% ) ,但并不能得到高衍射光强 ,若要得到高衍射光强 ,存在着最佳再现光强 (80 μW /cm2 )。实验证明了在BR D96N薄膜上可以实现全息图像记录。