齿孔酸对高尿酸血症黄嘌呤氧化酶活性的影响

采用紫外分光光度法检测齿孔酸在体外对黄嘌呤氧化酶的作用,并进行动力学研究探讨其作用机制;采用酵母联合氧嗪酸钾诱导高尿酸血症小鼠模型,观察齿孔酸对高尿酸血症小鼠血清尿酸水平、血清黄嘌呤氧化酶活性、肝脏黄嘌呤氧化酶活性及血糖血脂的影响。研究发现,齿孔酸体在外能抑制黄嘌呤氧化酶活性,降低高尿酸血症小鼠血清尿酸水平、血清黄嘌呤氧化酶活性、肝脏黄嘌呤氧化酶活性,同时明显降低空腹血糖、总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平,升高高密度脂蛋白胆固醇水平,提高口服糖耐受量。结果表明,齿孔酸是黄嘌呤氧化酶竞争性抑制剂,还能缓解高尿酸血症小鼠糖脂代谢紊乱,对高尿酸血症及痛风的防治具有潜在意义。     

一种提高水稻FISH检出率的新方法-RFLP混合标记

分别以水稻1号染色体上混合标记的8个紧密连锁的RFLP(平均约1.7kb)和5号染色体的BAC克隆44B4(137kb),以及12号染色体单个RFLPRG397（约1.5kb）为探针,在水稻染色体上进行了荧光原位杂交(FISH)。结果表明,RFLP混合标记杂交的检出率为27%,大大高于单个RFLP的检出率(7%)。其检出率虽然低于BAC克隆44B4(60%),但它具有程序简单易行的特点,使基因原位定位更加高效。由于水稻中与已知功能基因紧密连锁的RFLP标记具有数量丰富、分布密集等优势,揭示了混合标记的RFLP在禾本科植物同线性和共线性分析中的广阔应用前景。此外,混合标记的RFLP还可以用于染色体的准确识别和核型分析。 Abstract：Using mix-labeled 8 RFLPs (average length is about 1.7 kb) as a probe,the autho rs carried out fluorescence in situ hybridization in rice, and detected the sing le RFLP RZ397 (1.5 kb)and the BAC clone 44B4 (137 kb) simultaneously. The detec tion rate of mix-labeled RFLP was 27%, much higher than single-labeled RFLP (7%) , though lower than BAC clone (60%). Mix-labeled RFLP is an easy and effective m ethod to locate genes for its simplicity and sufficiency of RFLPs linked with th e functional genes. In addition, Mix-labeled RFLP groups can be used as effectiv e markers in karotype analysis of rice and the analysis of colinearity or synten y among cereals.     

VeA调控曲霉发育致病的研究进展

全局性调控因子Ve A仅在真菌中存在,具有保守性。Ve A参与细胞的多过程调控,包括发育分化、次生代谢、氧化胁迫应答、侵染宿主致毒致病等。文中总结曲霉Ve A的相关调控功能和作用机理研究,以利于防控真菌传播存活及致毒致病措施的设计,促进抗真菌作物的育种,减少作物被曲霉等真菌及毒素污染;还提出进一步开展ve A基因调控功能研究的方向和作为防控真菌污染靶位点的应用策略。     

A Strategy to Optimize the Oligo-Probes for Microarray-based Detection of Viruses

DNA microarrays have been acknowledged to represent a promising approach for the detection of viral pathogens. However, the probes designed for current arrays could cover only part of the given viral variants, that could result in false-negative or ambiguous data. If all the variants are to be covered, the requirement for more probes would render much higher spot density and thus higher cost of the arrays. Here we have developed a new strategy for oligonucleotide probe design. Using type I human immunodeficiency virus (HIV-1) tat gene as an example, we designed the array probes and validated the optimized parameters in silico. Results show that the oligo number is significantly reduced comparing with the existing methods, while specificity and hybridization efficiency remain intact. The adoption of this method in reducing the oligo numbers could increase the detection capacity for DNA microarrays, and would significantly lower the manufacturing cost for making array chips.