全文获取类型
收费全文 | 247篇 |
免费 | 11篇 |
专业分类
258篇 |
出版年
2024年 | 1篇 |
2023年 | 12篇 |
2022年 | 9篇 |
2021年 | 10篇 |
2020年 | 11篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 11篇 |
2016年 | 19篇 |
2015年 | 10篇 |
2014年 | 14篇 |
2013年 | 58篇 |
2012年 | 14篇 |
2011年 | 15篇 |
2010年 | 11篇 |
2009年 | 10篇 |
2008年 | 9篇 |
2007年 | 5篇 |
2006年 | 6篇 |
2004年 | 5篇 |
2003年 | 3篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1995年 | 1篇 |
1993年 | 2篇 |
1992年 | 1篇 |
排序方式: 共有258条查询结果,搜索用时 0 毫秒
101.
Canan Y. Karakaş Hande Tekarslan Şahin Benan İnan Didem Özçimen Yıldız Ö. Erginer 《Biotechnology progress》2019,35(6):e2876
Reactive oxygen species can bind protein, DNA, lipids, and carbohydrates and thus cause an oxidation reaction that induces various syndromes such as cardiovascular diseases, degenerative disease, and cancer types in the human body. Bioactive compounds, such as PUFA, EPA, DHA, and carotenoids in algae, have a chain ring and protect the tissue from chemical damage and reverse the symptoms of some diseases. Algal bioactives also have various biological properties such as anticoagulants, antiviral, antiangiogenic, antitumor, anti-inflammatory, antioxidant, antiproliferative, and immune modulation properties. This study aimed to show in vitro cytotoxic activity effect of Chlorella protothecoides and Nannochloropsis oculata microalgal extracts loaded nano–microparticles on A-172 (Homo sapiens brain glioblastoma) and HCT-116 (H. sapiens colon colorectal carcinoma) cell lines because of the increasing importance of algal biotechnology. MTT viability tests were performed on HUVEC, A172, and HCT 116 cells with particles obtained at optimum process parameters. The cell viability rates of encapsulated particles were also compared with pure algae extracts. Microalgal extracts loaded nano–micro particles showed very promising results for cytotoxic effect on cancer cells. 相似文献
102.
TUNA UYSAL KUDDISI ERTUĞRUL ALFONSO SUSANNA NÚRIA GARCİA‐JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2009,159(2):280-286
Twenty‐two chromosome counts are reported in 16 species, four subspecies and two varieties of the genus Centaurea. These are mostly Turkish local endemics of section Cheirolepis, a complicated group from the Eastern clade of the Jacea group. Twenty‐one reports are new. Prevalence of the basic chromosome number x = 9 among the eastern sections of the Jacea group is confirmed. © 2009 The Linnean Society of London, Botanical Journal of the Linnean Society, 2009, 159 , 280–286. 相似文献
103.
İpek Özcan Özlem Abacı Alev Haliki Uztan Buket Aksu Hayal Boyacıoğlu Tamer Güneri Özgen Özer 《AAPS PharmSciTech》2009,10(3):1024-1031
Chitosan-based carriers have important potential applications for the administration of drugs. In the present study, topical
gel formulations of terbinafine hydrochloride (T-HCl) were prepared using different types of chitosan at different molecular
weight, and the antifungal inhibitory activity was evaluated to suggest an effective formulation for the treatment of fungal
infections. The characteristics of gel formulations were determined with viscosity measurements and texture profile analysis.
Stability studies were performed at different temperatures during 3 months. The ex vivo permeation properties were studied through rat skin by using Franz diffusion cells. The antifungal inhibitory activity of
formulations on Candida species and filamentous fungi was also examined with agar-cup method. The microbiological assay was found suitable for determination
of in vitro antifungal activity of T-HCl. A marketed product was used to compare the results. The antifungal activity of T-HCl significantly
increased when it was introduced into the chitosan gels. A higher drug release and the highest zone of inhibition were obtained
from gels prepared with the lowest molecular weight chitosan (Protasan UP CL 213) compared to that of other chitosan gels
and marketed product. These results indicated the advantages of the suggested formulations for topical antifungal therapy
against Candida species and filamentous fungi. 相似文献
104.
Metin BÜLBÜL Olcay HİSAR Şükrü BEYDEMİR Mehmet ÇİFTÇİ Ö. İrfan KÜFREVIOĞLU 《Journal of enzyme inhibition and medicinal chemistry》2013,28(4):371-375
The in vitro and in vivo inhibitory effects of 5-(3α, 12α-dihydroxy-5-β-cholanamido)-1,3,4-thiadiazole-2-sulfonamide (1), 5-(3α, 7α, 12α-trihydroxy-5-β-cholanamido)-1,3,4-thiadiazole-2-sulfonamide (2), 5-(3α, 7α, 12α-triacetoxy-5-β-cholanamido)-1,3,4-thiadiazole-2-sulfonamide (3) and acetazolamide on rainbow trout (Oncorhynchus mykiss) (RT) erythrocyte carbonic anhydrase (CA) were investigated. The RT erythrocyte CA was obtained by affinity chromatography with a yield of 20.9%, a specific activity of 422.5?EU/mg protein and a purification of 222.4-fold. The purity of the enzyme was confirmed by SDS-PAGE. Inhibitory effects of the sulfonamides and acetazolamide on the RT erythrocyte CA were determined using the CO2-Hydratase method in vitro and in vivo studies. From in vitro studies, it was found that all the compounds inhibited CA. The obtained I50 value for the sulfonamides (1), (2) and (3) and acetazolamide were 0.83, 0.049, 0.82 and 0.052?μM, respectively. From in vivo studies, it was observed that CA was inhibited by the sulfonamides (1), (2) and (3) and acetazolamide. 相似文献
105.
Aim
The objective of this study was to examine whether MT plays a protective role against the damage in the liver by administering carbontetrachloride (CCl4) to rats.Main method
28 male Wistar albino (n = 28, 8 weeks old) rats have been used in the study. The rats were distributed into 4 groups according to their live weights. The groups were: (i) negative control (NC): normal water consuming group to which no CCl4 and milk thistle (MT) is administered; (ii) positive control (PC): normal water consuming group to which no CCl4 is administered but MT is administered; (iii) CCl4 group: normal water consuming and group to which CCl4 is administered (2 ml/kg live weight, ip); and (iv) CCl4 + MT group: CCl4 and MT administered group (2 ml/kg live weight, ip). Caspase-3, caspase-9, bax, and bcl-2 protein syntheses were examined via western blotting. MDA determination in liver tissue was made using spectrophotometer.Key findings
MDA amount has decreased in the CCl4 + MT group in comparison to CCl4 group whereas caspase-3 and caspase-9 has increased and bax and bcl-2 has decreased.Significance
These results show that MT protects the liver against oxidative damage. 相似文献106.
İbrahim Esirden Muhammet Tanç Claudiu T. Supuran Muharrem Kaya 《Bioorganic & medicinal chemistry letters》2017,27(1):86-89
The synthesis of novel tetrazole/sulfonamide derivatives based on octahydroacridine, xanthene and chromene scaffold by using microwave (MW) assisted techniques is reported in this study. These synthesized hybrid compounds were assayed for the inhibition of carbonic anhydrase (CA, EC 4.2.1.1). The inhibitory activities were determined against three cytosolic human isoforms (hCA I, II and VII) and one membrane-associated (hCA IV) isoform. Some of the newly synthesized sulfonamides showed micromolar to nanomolar inhibitory activity against these enzymes. 相似文献
107.
Many of the cancer cells produce energy with accelerated glycolysis and perform lactic acid production even under normoxic conditions called the “Warburg effect”. Metabolism can directly or indirectly regulate the apoptotic mechanism so that cancer cells take advantage of reprogrammed metabolism to avoid apoptosis. The aim of this study is to examine the mechanism of apoptosis by incubating human lung carcinoma cells (A549) under different metabolic conditions in hypoxia or normoxia environments. A549 cells were incubated in the normoxic or hypoxic condition that contained 5 mM glucose (Glc 5), 25 mM glucose (Glc 25), or 10 mM galactose (OXPHOS/aglycemic), and the mechanism of apoptosis was investigated. In the hypoxia condition, the rate of early apoptosis in aglycemic OXPHOS cells was increased (15.5% ±7.1). In addition, the activity of caspase-3 (6.1% ± 0.9), caspase-9 (30.4% ± 0.9), and cytochrome c expression level increased; however, the mitochondrial membrane potential (51.9% ± 0.4) was found to be decreased. Changing the amount of oxygen in glycolytic cells had no effect on apoptosis. However, it has been determined that apoptosis is stimulated under hypoxia conditions in aglycemic cells in which galactose is used instead of glucose. Considering that the majority of cancer cells are hypoxic, these data are important in determining targets in therapeutic intervention. 相似文献
108.
We investigated the microscopic structure of transverse sections of the oculomotor, trochlear and abducens nerves of Arabian foals using stereological methods. Bilateral nerve pairs from 2-month-old female Arabian foals were analyzed. The tissues were embedded in plastic blocks, then 1 µm thick sections were cut and stained with osmium tetroxide and methylene blue-azure II. Stereology was performed using light microscopy. Morphometry showed that the right and left pairs of nerves were similar. The transverse sectional areas of the oculomotor, trochlear and abducens nerves were 1.93 ± 0.19 mm2, 0.32 ± 0.06 mm2 and 0.70 ± 0.08 mm2, respectively. The oculomotor nerve exhibited a significantly greater number of myelinated axons (16755 ± 1279) and trochlear (2656 ± 494) and the abducens nerves (4468 ± 447). The ratio of the axon diameter to myelinated nerve fiber diameter was 0.58, 0.55 and 0.55 for the oculomotor, trochlear and abducens nerves, respectively. Of the three nerves studied, the abducens nerve exhibited the greatest nerve fiber area, myelin area, nerve and axon diameters, and myelin thickness. The ratio of small myelinated nerve fibers was greatest in the oculomotor nerve. 相似文献
109.
Polyphenol oxidase (PPO) of nettle (Urtica dioica L.) was extracted and purified through (NH4)2SO4 precipitation, dialysis, and CM-Sephadex ion-exchange chromatography and was used for its characterization. The PPO showed activity to catechol, 4-methylcatechol, L-3,4-dihydroxyphenylalanine (L-DOPA), L-tyrosine, p-cresol, pyrogallol, catechin and trans-cinnamic acid. For each of these eight substrates, optimum conditions such as pH and temperature were determined and L-tyrosine was found to be one of the most suitable substrates. Optimum pH and temperature were found at pH 4.5 and 30°C respectively and Km and Vmax values were 7.90?×?10?4?M, and 11290?EU/mL for with L-tyrosine as substrate. The inhibitory effect of several inhibitors, L-cysteine chloride, sodium azide, sodium cyanide, benzoic acid, salicylic acid, L-ascorbic acid, glutathione, thiourea, sodium diethyl dithiocarbamate, β-mercaptoethanol and sodium metabisulfite were tested. The most effective was found to be sodium diethyl dithiocarbamate which acted as a competitive inhibitor with a Ki value of 1.79?×?10?9?M. In addition one isoenzyme of PPO was detected by native polacrylamide slab gel electrophoresis. 相似文献
110.
Hosseinpour Arash Ilhan Emre Özkan Güller Öztürk Halil İbrahim Haliloglu Kamil Cinisli Kağan Tolga 《Journal of plant biochemistry and biotechnology.》2022,31(4):751-764
Journal of Plant Biochemistry and Biotechnology - Wheat is the second important cereal crop worldwide due to nutritional composition and role in meeting daily energy needs. Salinity is an abiotic... 相似文献