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931.
Having historically been abundant throughout Europe, the house sparrow (Passer domesticus) has in recent decades suffered severe population declines in many urban and rural areas. The decline in rural environments
is believed to be caused by agricultural intensification, which has resulted in landscape simplification. We used giving-up
densities (GUDs) of house sparrows feeding in artificial food patches placed in farmlands of southern Sweden to determine
habitat quality during the breeding season at two different spatial scales: the landscape and the patch scale. At the landscape
scale, GUDs were lower on farms in homogeneous landscapes dominated by crop production compared to more heterogeneous landscapes
with mixed farming or animal husbandry. At the patch level, feeding patches with a higher predation risk (caused by fitting
a wall to the patch to obstruct vigilance) had higher GUDs. In addition, GUDs were positively related to population size,
which strongly implies that GUDs reflect habitat quality. However, the increase followed different patterns in homogeneous
and heterogeneous landscapes, indicating differing population limiting mechanisms in these two environments. We found no effect
of the interaction between patch type and landscape type, suggesting that predation risk was similar in both landscape types.
Thus, our study suggests that simplified landscapes constitute a poorer feeding environment for house sparrows during breeding,
that the population-regulating mechanisms in the landscapes differ, but that predation risk is the same across the landscape
types. 相似文献
932.
Pyrosequencing-derived bacterial, archaeal, and fungal diversity of spacecraft hardware destined for Mars 总被引:2,自引:0,他引:2
La Duc MT Vaishampayan P Nilsson HR Torok T Venkateswaran K 《Applied and environmental microbiology》2012,78(16):5912-5922
Spacecraft hardware and assembly cleanroom surfaces (233 m(2) in total) were sampled, total genomic DNA was extracted, hypervariable regions of the 16S rRNA gene (bacteria and archaea) and ribosomal internal transcribed spacer (ITS) region (fungi) were subjected to 454 tag-encoded pyrosequencing PCR amplification, and 203,852 resulting high-quality sequences were analyzed. Bioinformatic analyses revealed correlations between operational taxonomic unit (OTU) abundance and certain sample characteristics, such as source (cleanroom floor, ground support equipment [GSE], or spacecraft hardware), cleaning regimen applied, and location about the facility or spacecraft. National Aeronautics and Space Administration (NASA) cleanroom floor and GSE surfaces gave rise to a larger number of diverse bacterial communities (619 OTU; 20 m(2)) than colocated spacecraft hardware (187 OTU; 162 m(2)). In contrast to the results of bacterial pyrosequencing, where at least some sequences were generated from each of the 31 sample sets examined, only 13 and 18 of these sample sets gave rise to archaeal and fungal sequences, respectively. As was the case for bacteria, the abundance of fungal OTU in the GSE surface samples dramatically diminished (9× less) once cleaning protocols had been applied. The presence of OTU representative of actinobacteria, deinococci, acidobacteria, firmicutes, and proteobacteria on spacecraft surfaces suggests that certain bacterial lineages persist even following rigorous quality control and cleaning practices. The majority of bacterial OTU observed as being recurrent belonged to actinobacteria and alphaproteobacteria, supporting the hypothesis that the measures of cleanliness exerted in spacecraft assembly cleanrooms (SAC) inadvertently select for the organisms which are the most fit to survive long journeys in space. 相似文献
933.
934.
L Benga WP Benten E Engelhardt H Christensen M Sager 《Journal of microbiological methods》2012,90(3):342-349
The internal transcribed spacer (ITS) regions of members of Pasteurellaceae isolated from rodents, including the [Pasteurella] pneumotropica biotypes Jawetz and Heyl, [Actinobacillus] muris, "Hemophilus influenzaemurium" and Bisgaard taxon 17 were studied and their feasibility to discriminate these species was analyzed. The reference strains of all species analyzed showed unique species-specific ITS patterns which were further present in 49 clinical isolates of [P.] pneumotropica biotypes Jawetz and Heyl and [A.] muris allowing their identification by comparison to the reference strains pattern. Sequence analysis of the amplified fragments revealed in all species, with exception of "H. influenzaemurium", a larger ITS(ile+ala) which contained the genes for tRNA(Ile(GAU)) and tRNA(Ala(UGC)) and a smaller ITS(glu) with the tRNA(Glu(UUC)) gene. "H. influenzaemurium" revealed two each of the larger and respectively the smaller ITS fragments. Both the length and the sequence of each ITS type were highly conserved within the [P.] pneumotropica biotypes Jawetz and Heyl and [A.] muris strains tested. On the contrary, ITS sequences revealed significant interspecies variations with identity levels ranging from 61.2 to 89.5% for ITS(ile+ala) and 56.5 to 86.8% for ITS(glu). Sequences regions with significant interspecies variation but highly conserved within the species were identified and might be used to design probes for the identification of rodent Pasteurellaceae to the species level. 相似文献
935.
M. I. Pedersen N. Jepsen K. Aarestrup A. Koed S. Pedersen F. Økland 《Zeitschrift fur angewandte Ichthyologie》2012,28(2):189-193
The aim of this study was to assess escapement success of silver eels, Anguilla anguilla (L.), in a lowland river while passing a reservoir and a hydropower station. It was hypothesized that passage success would be lowest at the hydropower station and that survival and migration speed would be highest in the free‐flowing river section upstream the reservoir. Forty‐five female silver eels 56–86 cm in length were tagged with acoustic transmitters and released in November 2006. Their migration was monitored via automatic listening stations (ALS) in various sections of the river, covering a total migration distance of 64 km. Survival and progression rate of downstream migration was highest in the upstream river section and significantly lower in the reservoir. The eels apparently had trouble finding their way past the turbines and spent between 1.5 and 35 h in the forebay. The results show that within the study period, only 23% of the tagged eels reached the tidal limit, mainly due to difficulties in passing the hydropower dam. With such high loss‐rates, the escapement goals set in the management plan cannot be achieved. 相似文献
936.
Anne C. Lang Werner H?rdtle Helge Bruelheide Wenzel Kr?ber Matthias Schr?ter Henrik von Wehrden Goddert von Oheimb 《Ecological Research》2012,27(1):181-189
The aim of this study was to analyse the relation of horizontal and vertical canopy structure to tree functional diversity
of a highly diverse subtropical broad-leaved slope forest, stratified for different successional stages. This is of particular
interest because many key ecosystem processes and functions are related to the arrangement of forest canopies. We assessed
the effect of stand-related functional diversity (FDQ, measured as Rao’s quadratic entropy of leaf traits), together with other environmental variables on horizontal [measured
as relative crown projection areas (CPAr)] and vertical [relative crown overlap, coefficients of variation (CV) of crown positioning variables] structure of the upper
canopy at the local neighbourhood level. The analyses with mixed effects models revealed a negative relation (p = 0.025; estimate −0.07) between FDQ and CPAr. No significant effect of FDQ on vertical canopy structure has been found (p > 0.05). The findings are discussed with regard to resource partitioning and niche differentiation of canopy and sub-canopy
species. Successional stage positively impacted the CV of crown length (p = 0.019; estimate 0.03) but did not affect other response variables. The sloping terrain strongly influenced vertical canopy
structure as revealed by the significant effect of slope inclination on CV of crown length (p = 0.004; estimate −0.05) and of slope aspect on CV of mean crown height (p = 0.036; estimate −0.03). The high complexity of vertical crown positioning depending on the heterogeneous sloping terrain
of the study area may have obscured relations of FDQ to vertical canopy structure. 相似文献
937.
In marine food web studies, stable isotopes of nitrogen (δ15N) and carbon (δ13C) are widely used to estimate organisms’ trophic levels (TL) and carbon sources, respectively. For smaller organisms, whole
specimens are commonly analyzed. However, this “bulk method” involves several pitfalls since different tissues may fractionate
stable isotopes differently. We compared the δ15N and δ13C values of exoskeleton versus soft tissue, in relation to whole specimens, of three common Arctic amphipods in Svalbard waters:
the benthic Anonyx nugax, the sympagic (ice-associated) Gammarus wilkitzkii and the pelagic Themisto libellula. The δ15N values of the exoskeletons were significantly lower than those of the soft tissues for A. nugax (10.5 ± 0.7‰ vs. 15.7 ± 0.7‰), G. wilkitzkii (3.3 ± 0.3‰ vs. 8.3 ± 0.4‰) and T. libellula (8.6 ± 0.3‰ vs.10.8 ± 0.3‰). The differences in δ13C values between exoskeletons and soft tissues were insignificant, except for A. nugax (−21.2 ± 0.2‰ vs. −20.3 ± 0.2‰, respectively). The δ15N values of whole organisms were between those of the exoskeletons and the soft tissues, being similarly enriched in 15N as the exoskeletons (except G. wilkitzkii) and depleted in 15N by 1.2–3.7‰ compared to the soft tissues. The δ15N-derived TLs of the soft tissues agreed best with the known feeding preferences of the three amphipods, which suggest a potential
underestimation of 0.5–1.0 TL when stable isotope analyses are performed on whole crustaceans with thick exoskeletons. The
insignificant or small differences in δ13C values among exoskeletons, soft tissues and whole specimens, however, suggest low probability for misinterpretations of
crustaceans’ primary carbon source in marine ecosystems with distinctly different δ13C-carbon sources. 相似文献
938.
Pärn H Ringsby TH Jensen H Sæther BE 《Proceedings. Biological sciences / The Royal Society》2012,279(1726):144-152
Dispersal plays a key role in the response of populations to climate change and habitat fragmentation. Here, we use data from a long-term metapopulation study of a non-migratory bird, the house sparrow (Passer domesticus), to examine the influence of increasing spring temperature and density-dependence on natal dispersal rates and how these relationships depend on spatial variation in habitat quality. The effects of spring temperature and population size on dispersal rate depended on the habitat quality. Dispersal rate increased with temperature and population size on poor-quality islands without farms, where house sparrows were more exposed to temporal fluctuations in weather conditions and food availability. By contrast, dispersal rate was independent of spring temperature and population size on high-quality islands with farms, where house sparrows had access to food and shelter all the year around. This illustrates large spatial heterogeneity within the metapopulation in how population density and environmental fluctuations affect the dispersal process. 相似文献
939.
Sara Fuentes Karin Ljung Karim Sorefan Elizabeth Alvey Nicholas P. Harberd Lars ?stergaard 《The Plant cell》2012,24(10):3982-3996
Fruit growth and development depend on highly coordinated hormonal activities. The phytohormone gibberellin (GA) promotes growth by inducing degradation of the growth-repressing DELLA proteins; however, the extent to which DELLA proteins contribute to GA-mediated gynoecium and fruit development remains to be clarified. Here, we provide an in-depth characterization of the role of DELLA proteins in Arabidopsis thaliana fruit growth. We show that DELLA proteins are key regulators of reproductive organ size and important for ensuring optimal fertilization. We demonstrate that the seedless fruit growth (parthenocarpy) observed in della mutants can be directly attributed to the constitutive activation of GA signaling. It has been known for >75 years that another hormone, auxin, can induce formation of seedless fruits. Using mutants with complete lack of DELLA activity, we show here that auxin-induced parthenocarpy occurs entirely through GA signaling in Arabidopsis. Finally, we uncover the existence of a DELLA-independent GA response that promotes fruit growth. This response requires GIBBERELLIN-INSENSITIVE DWARF1–mediated GA perception and a functional 26S proteasome and involves the basic helix-loop-helix protein SPATULA as a key component. Taken together, our results describe additional complexities in GA signaling during fruit development, which may be particularly important to optimize the conditions for successful reproduction. 相似文献
940.
Candidate states of Helicobacter pylori's genome-scale metabolic network upon application of "loop law" thermodynamic constraints 下载免费PDF全文
Constraint-based modeling has proven to be a useful tool in the analysis of biochemical networks. To date, most studies in this field have focused on the use of linear constraints, resulting from mass balance and capacity constraints, which lead to the definition of convex solution spaces. One additional constraint arising out of thermodynamics is known as the "loop law" for reaction fluxes, which states that the net flux around a closed biochemical loop must be zero because no net thermodynamic driving force exists. The imposition of the loop-law can lead to nonconvex solution spaces making the analysis of the consequences of its imposition challenging. A four-step approach is developed here to apply the loop-law to study metabolic network properties: 1), determine linear equality constraints that are necessary (but not necessarily sufficient) for thermodynamic feasibility; 2), tighten V(max) and V(min) constraints to enclose the remaining nonconvex space; 3), uniformly sample the convex space that encloses the nonconvex space using standard Monte Carlo techniques; and 4), eliminate from the resulting set all solutions that violate the loop-law, leaving a subset of steady-state solutions. This subset of solutions represents a uniform random sample of the space that is defined by the additional imposition of the loop-law. This approach is used to evaluate the effect of imposing the loop-law on predicted candidate states of the genome-scale metabolic network of Helicobacter pylori. 相似文献