Isolation and identification of alkaline protease producer halotolerantBacillus licheniformis strain BA17

An alkaline protease producerBacillus licheniformis strain was isolated from Van Lake in Turkey. The strain is Gram positive, aerobic, motile, sporulating rod-shaped bacterium. Spores were ellipsoidal and positioned central in nonswollen sporangium. The cells were able to grow well at a pH range of 5.7–10. The optimal growth temperature was found to be 37 °C. Growth at a wide range of NaCl concentration (from 0 to 20%) showed that BA17 is halotolerant. Main fatty acid composition of BA17 was anteiso-C15:0 and iso-C15∶0. The strain was presumptively identified asB. licheniformis according to 16S rDNA gene sequence analysis. The most appropriate medium for the growth and protease production is composed of 0.5% yeast extract, 0.5% NaNO₃, 0.02% MgSO₄\7H₂O, 0.1% K₂HPO₄ and 0.5% maltose. The optimum temperature and pH of the alkaline protease of strain BA17 were found to be 60 °C and pH 11, respectively. The activity was completely lost in the presence of PMSF, suggesting that the preparation contains serine-alkaline protease(s).     

Screening Radiolocation Datasets for Movement Strategies With Time Series Segmentation

ABSTRACT Classical home range analysis is tailored to meet requirements of data with few points per individual with relatively large intervals between observations. The swift rise in Global Positioning System (GPS)-based studies requires the development of new analytical approaches because GPS data allow for more detailed analysis in time and space. The amount of data derived from GPS studies enhances the potential to more accurately separate movement strategies. We present a general, simple, conceptual approach to using large movement datasets to automatically screen and delimit spatial and temporal home ranges of individuals and movement strategies using time series segmentation. We used GPS data for moose (Alces alces) from a boreal Swedish population as an example. We tested predictions that our screening method could separate seasonal migration from dispersal and nomadic strategies by the movement profile, which includes several dimensions. Our analysis showed that broad strategies were detected using our simple analytical approach, which speeds up use of GPS data for management and research because the method can be used to calculate more objective spatial and temporal activity ranges in relation to movement strategies. Our examples illustrate the importance of using the time stamp on location data in describing home ranges and movements.     

Nisin production in a chitin-including continuous fermentation system with Lactococcus lactis displaying a cell wall chitin-binding domain

The limiting factors in the continuous production of nisin are high amount of biomass loss and low dilution rate application. In this study, a chitin-including continuous nisin fermentation system (CICON-FER) was constructed for high volumetric nisin production using nisin producer L. lactis displaying cell wall chitin-binding domain (ChBD) together with chitin in the reactor. In this respect, the highest binding conditions of relevant L. lactis cells to chitin were determined. Then the chitin flakes carrying nisin-producing L. lactis cells were used within the CICON-FER system at different dilution rates (0.1–0.9 h^?1) and initial glucose concentrations (20–60 g l^?1). The results revealed that the pH 7 conditions and the use of 100 mM sodium phosphate buffer with 0.1 % Tween 20 and Triton X-100 significantly increased the binding capacity of ChBD displaying L. lactis cells to chitin. The constructed CICON-FER system maintained the presence of the ChBD surface displaying L. lactis cells in the reactor system until 0.9 h^?1 dilution rate that resulted in a considerably high level of volumetric nisin production and productivity (10,500 IU ml^?1 and 9,450 IU ml^?1 h^?1, respectively) with the combination of a 0.9-h^?1 dilution rate and a 40-g l^?1 initial glucose concentration. In conclusion, an innovative nisin fermentation system that yielded the highest nisin production thus far and that was feasible for industrial application was created.     

Analysis of the fruit surfaces in Bupleurum L. (Umbelliferae) with SEM

20 Bupleurum L. taxa were investigated for micromorphological typology of the fruit surfaces with SEM. Considerably different types of sculpturing at species and subspecies level and similarities between related taxa were observed. A dichotomic key was prepared using the diagnostic surface patterns. Reticulate (sect. Bupleurum), rugose (sect. Aristata Godron subsect. Aristata) and rugulose-colliculate-verrucate (subsect. Juncea Briq.) types of surface structures were distinguished. Micromorphological characteristics may be valuable additional taxonomical criteria for the delimitation of infrageneric groups of species.     

Spectral sensitivity of the green photoreceptor of winged pea aphids

Intracellular recordings are obtained from photoreceptors in the retina of winged (alate) pea aphids Acyrthosiphon pisum (Harris). The responses to monochromatic light, applied in 10‐nm steps over the range 320–650 nm, reveal that all recordings are from green receptors and the spectral sensitivity function of these photoreceptors peaks at 518 nm. A comparison between the spectral sensitivity of the green receptors and extracellular electroretinogram recordings suggests that additional sensitivity to the short‐wavelength light (ultraviolet and/or blue) is also likely to be present in the compound eye of pea aphids. An analysis of the pea aphid genome, comparing its translated nucleotide sequences with the those of the opsin genes of other insect species, supports this electrophysiological finding, although it could not be established whether A. pisum, in addition to the green receptor, has both blue and ultraviolet receptors in the compound eye. The implications of these results for the visual ecology of herbivorous insects are discussed.     

J deflections on ECG in severe hypothermia and hypokalaemia: a case report

The J wave, also known as Osborn wave, is a deflection that can be observed on the surface ECG as a late delta wave, seen at the end of the QRS complex. In this case, a 75-year-old woman, after 1 day of continuous haemodialysis, showed a marked hypothermia (28.5°C) and severe hypokalaemia (1.7 mEq/l). Bradycardia was seen on the monitor and J waves were recognised on the ECG recording. After appropriate replacement of potassium and treatment of hypothermia, the J waves disappeared spontaneously.     

Multiple Pleistocene refugia and Holocene range expansion of an abundant southwestern American desert plant species (Melampodium leucanthum,Asteraceae)

Pleistocene climatic fluctuations had major impacts on desert biota in southwestern North America. During cooler and wetter periods, drought‐adapted species were isolated into refugia, in contrast to expansion of their ranges during the massive aridification in the Holocene. Here, we use Melampodium leucanthum (Asteraceae), a species of the North American desert and semi‐desert regions, to investigate the impact of major aridification in southwestern North America on phylogeography and evolution in a widespread and abundant drought‐adapted plant species. The evidence for three separate Pleistocene refugia at different time levels suggests that this species responded to the Quaternary climatic oscillations in a cyclic manner. In the Holocene, once differentiated lineages came into secondary contact and intermixed, but these range expansions did not follow the eastwardly progressing aridification, but instead occurred independently out of separate Pleistocene refugia. As found in other desert biota, the Continental Divide has acted as a major migration barrier for M. leucanthum since the Pleistocene. Despite being geographically restricted to the eastern part of the species’ distribution, autotetraploids in M. leucanthum originated multiple times and do not form a genetically cohesive group.