全文获取类型
收费全文 | 4908篇 |
免费 | 304篇 |
专业分类
5212篇 |
出版年
2024年 | 7篇 |
2023年 | 44篇 |
2022年 | 65篇 |
2021年 | 153篇 |
2020年 | 99篇 |
2019年 | 117篇 |
2018年 | 159篇 |
2017年 | 158篇 |
2016年 | 182篇 |
2015年 | 234篇 |
2014年 | 312篇 |
2013年 | 382篇 |
2012年 | 453篇 |
2011年 | 505篇 |
2010年 | 380篇 |
2009年 | 238篇 |
2008年 | 282篇 |
2007年 | 185篇 |
2006年 | 170篇 |
2005年 | 153篇 |
2004年 | 124篇 |
2003年 | 114篇 |
2002年 | 108篇 |
2001年 | 59篇 |
2000年 | 67篇 |
1999年 | 47篇 |
1998年 | 26篇 |
1997年 | 17篇 |
1996年 | 19篇 |
1995年 | 11篇 |
1994年 | 23篇 |
1993年 | 19篇 |
1992年 | 33篇 |
1991年 | 26篇 |
1990年 | 21篇 |
1989年 | 11篇 |
1988年 | 19篇 |
1987年 | 16篇 |
1985年 | 13篇 |
1984年 | 14篇 |
1983年 | 10篇 |
1982年 | 9篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1975年 | 9篇 |
1974年 | 13篇 |
1973年 | 9篇 |
1972年 | 10篇 |
1971年 | 16篇 |
1966年 | 6篇 |
排序方式: 共有5212条查询结果,搜索用时 15 毫秒
71.
Carvalho Rodrigo A. Teresa Fabrício B. Tejerina-Garro Francisco L. 《Hydrobiologia》2021,848(2):515-529
Hydrobiologia - We investigated how the riverine network influences taxonomic and functional beta diversity patterns of fish assemblages in the mainstem/headwater (lateral) and upstream/downstream... 相似文献
72.
Lêda N. Regis Ridelane Veiga Acioli José Constantino Silveira Jr. Maria Alice Varjal Melo-Santos Wayner Vieira Souza Candida M. Nogueira. Ribeiro Juliana C. Serafim. da Silva Antonio Miguel Vieira Monteiro Cláudia M. F. Oliveira Rosangela M. R. Barbosa Cynthia Braga Marco Aurélio Benedetti Rodrigues Marilú Gomes N. M. Silva Paulo Justiniano Ribeiro Jr. Wagner Hugo Bonat Liliam César de Castro Medeiros Marilia Sa Carvalho André Freire Furtado 《PloS one》2013,8(7)
Aedes aegypti has developed evolution-driven adaptations for surviving in the domestic human habitat. Several trap models have been designed considering these strategies and tested for monitoring this efficient vector of Dengue. Here, we report a real-scale evaluation of a system for monitoring and controlling mosquito populations based on egg sampling coupled with geographic information systems technology. The SMCP-Aedes, a system based on open technology and open data standards, was set up from March/2008 to October/2011 as a pilot trial in two sites of Pernambuco -Brazil: Ipojuca (10,000 residents) and Santa Cruz (83,000), in a joint effort of health authorities and staff, and a network of scientists providing scientific support. A widespread infestation by Aedes was found in both sites in 2008–2009, with 96.8%–100% trap positivity. Egg densities were markedly higher in SCC than in Ipojuca. A 90% decrease in egg density was recorded in SCC after two years of sustained control pressure imposed by suppression of >7,500,000 eggs and >3,200 adults, plus larval control by adding fishes to cisterns. In Ipojuca, 1.1 million mosquito eggs were suppressed and a 77% reduction in egg density was achieved. This study aimed at assessing the applicability of a system using GIS and spatial statistic analysis tools for quantitative assessment of mosquito populations. It also provided useful information on the requirements for reducing well-established mosquito populations. Results from two cities led us to conclude that the success in markedly reducing an Aedes population required the appropriate choice of control measures for sustained mass elimination guided by a user-friendly mosquito surveillance system. The system was able to support interventional decisions and to assess the program’s success. Additionally, it created a stimulating environment for health staff and residents, which had a positive impact on their commitment to the dengue control program. 相似文献
73.
Unfolding kinetics of beta-lactoglobulin induced by surfactant and denaturant: a stopped-flow/fluorescence study 下载免费PDF全文
The beta-->alpha transition of beta-lactoglobulin, a globular protein abundant in the milk of several mammals, is investigated in this work. This transition, induced by the cationic surfactant dodecyltrimethylammonium chloride (DTAC), is accompanied by partial unfolding of the protein. In this work, unfolding of bovine beta-lactoglobulin in DTAC is compared with its unfolding induced by the chemical denaturant guanidine hydrochloride (GnHCl). The final protein states attained in the two media have quite different secondary structure: in DTAC the alpha-helical content increases, leading to the so-called alpha-state; in GnHCl the amount of ordered secondary-structure decreases, resulting in a random coil-rich final state (denatured, or D, state). To obtain information on both mechanistic routes, in DTAC and GnHCl, and to characterize intermediates, the kinetics of unfolding were investigated in the two media. Equilibrium and kinetic data show the partial accumulation of an on-pathway intermediate in each unfolding route: in DTAC, an intermediate (I(1)) with mostly native secondary structure but loose tertiary structure appears between the native (beta) and alpha-states; in GnHCl, another intermediate (I(2)) appears between states beta and D. Kinetic rate constants follow a linear Chevron-plot representation in GnHCl, but show a more complex mechanism in DTAC, which acts like a stronger binding species. 相似文献
74.
Edleide L. da Silva Cenira M. de Carvalho Ruth R. do Nascimento Adriana L. Mendonça Carlos E. da Silva Gláucia B. Gonçalves Maria do Rosário T. de Freitas & Antônio E. G. Sant'Ana 《Ethology : formerly Zeitschrift fur Tierpsychologie》2006,112(10):971-976
The Annona fruit borer, Cerconota anonella, causes significant damage to the fruits of Annona squamosa (custard apple) and A. muricata (soursop). The methods currently available for the control of this pest are costly and new techniques, possibly involving the use of pheromones for trapping or disrupting the mating cycle of the insect are required. In order to provide the basic information required for the development of new control systems, the reproductive behaviour of the moth was observed under laboratory conditions. The calling and courtship behaviours exhibited by virgin females and males of C. anonella commenced at the eighth hour of the scotophase and continued for a 3.5‐h period. Males were attracted by conspecific females as young as 1 d old, and showed a courtship behaviour composed of three steps: antennation, wing fanning and short flights. Mating mainly occurred when both males and females were between 2 and 5 d old, but maximum activity was observed on the third day after emergence. Receptive females elevated their wings, showing their abdomens where the abdominal hairpencils were already exposed. As part of the courtship repertoire and immediately prior to copula, males performed pronounced sideways movements of their abdomens, a behaviour that appears to be exclusive to C. anonella. 相似文献
75.
Marcelo R. de Carvalho Flávio A. Bockmann Dalton S. Amorim Carlos Roberto F. Brandão Mário de Vivo José L. de Figueiredo Heraldo A. Britski Mário C. C. de Pinna Naércio A. Menezes Fernando P. L. Marques Nelson Papavero Eliana M. Cancello Jorge V. Crisci John D. McEachran Robert C. Schelly John G. Lundberg Anthony C. Gill Ralf Britz Quentin D. Wheeler Melanie L. J. Stiassny Lynne R. Parenti Larry M. Page Ward C. Wheeler Julián Faivovich Richard P. Vari Lance Grande Chris J. Humphries Rob DeSalle Malte C. Ebach Gareth J. Nelson 《Evolutionary biology》2007,34(3-4):140-143
76.
P Venault L Prado de Carvalho C L Brown R H Dodd J Rossier G Chapouthier 《Life sciences》1986,39(12):1093-1100
Certain pharmacological properties of methyl beta-carboline-3-carboxylate (beta-CCM), a benzodiazepine receptor ligand, have been investigated in chicks. Although beta-CCM has been established previously as an "inverse agonist" of benzodiazepine receptors in rodents, having effects opposite to those of benzodiazepines in a variety of tests, in chicks this compound had a different pharmacological profile. Firstly, in contrast to the overt convulsant action of beta-CCM in other species, beta-CCM (0.05-40 mg/kg) did not produce convulsions by itself in chicks, but it was only proconvulsant. Secondly and most surprisingly, beta-CCM, like diazepam, produced in chicks a sedation which could be blocked by the benzodiazepine receptor antagonist Ro 15-1788. Thus it appears that beta-CCM can function both as an agonist and as an inverse agonist in this animal. 相似文献
77.
Cryptic diversity in the lizard genus Plica (Squamata): phylogenetic diversity and Amazonian biogeography 下载免费PDF全文
Deyla Paula de Oliveira Vinícius Tadeu de Carvalho Tomas Hrbek 《Zoologica scripta》2016,45(6):630-641
Intra‐ and interspecific genetic diversity of the lizard species Plica plica (9 localities) and Plica umbra (19 localities) from the Brazilian Amazon was analysed using two mitochondrial (16S rDNA and CO1) and one nuclear (prolactin receptor – PRLR) genes. We generated a maximum‐likelihood and Bayesian hypotheses of phylogenetic relationships, and using the bPTP and ABGD lineage delimiting methods inferred the most likely number of lineages within each species. Both methods delimited five distinct lineages in Plica plica and six lineages within Plica umbra. The nominal subspecies of Plica umbra was comprised of one lineage, while Plica umbra ochrocollaris was comprised of five lineages. In majority of the cases, lineages were restricted to the interfluves of major Amazonian rivers, and different lineages occupied distinct areas of endemism. Phylogenetic relationships of the lineages are largely concordant with the hypothesized formation of the areas of endemism. The geographic structuring of the clades and the delimitation of these clades as distinct lineages suggest the possibility that these lineages represent species. If the observed diversity of lineages within the genus Plica is characteristic of squamate reptiles of the Amazon region, the diversity of squamates is grossly underestimated. 相似文献
78.
Contemporary patterns of genetic diversity of cedrela fissilis offer insight into the shaping of seasonal forests in eastern South America 下载免费PDF全文
79.
Júlia Cristina Cardoso Carraro Maria Luisa Mansego Fermin Ignacio Milagro Larissa Oliveira Chaves Fernanda Carvalho Vidigal Josefina Bressan 《Biomarkers》2016,21(7):625-632
We analyzed whether global and inflammatory genes methylation can be early predictors of metabolic changes and their associations with the diet, in a cross-sectional study (n?=?40). Higher global methylation was associated to adiposity, insulin resistance, and lower quality of the diet. Methylation of IL-6, SERPINE1 and CRP genes was related to adiposity traits and macronutrients intake. SERPINE1 hypermethylation was also related to some metabolic alterations. CRP methylation was a better predictor of insulin resistance than CRP plasma concentrations. Global and inflammatory gene promoter hypermethylation can be good early biomarkers of adiposity and metabolic changes and are associated to the quality of the diet. 相似文献
80.
Ascorbate oxidase is present in homogenates of the flesh of Cucurbita maxima fruits. Its activity is independent of ascorbate concentration over th 相似文献