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101.
Juliana Dänhardt Martin Green Åke Lindström Maj Rundlöf Henrik G. Smith 《Oikos》2010,119(7):1114-1125
Agricultural intensification in Europe has affected farmland bird populations negatively, both during summer and winter. Although the migratory period poses separate challenges on birds than breeding and wintering, the consequences of farming practices for birds during migration remain poorly investigated. We monitored abundance and species richness of migratory birds in autumn at matched pairs of organic and conventional farms situated either in intensively farmed open plains (homogeneous landscapes) or in small‐scale farming landscapes (heterogeneous landscapes) in southern Sweden. Total bird density did not differ between landscape types but was marginally higher on organic compared to conventional farms. When including taxonomic status in the model (passerines vs non‐passerines), we found significantly more birds on organic farms, and more non‐passerines in the homogeneous landscapes. The effect of farming practice and landscape type on density differed between functional groups. Omnivore density was higher in the homogeneous landscapes, and invertebrate feeders were marginally more abundant on organic farms. The effects of farming practice on the overall species richness and on the density of granivorous birds were landscape dependent. In the homogeneous landscapes, organic farms held a higher number of species and density of granivorous birds than conventional farms, but there was no such difference in the heterogeneous landscapes. Thus, organic farming can enhance abundance and species richness of farmland birds during migration, but the effect differs between landscape types and species. The effectiveness of organic farming was highest in the homogeneous landscape making it important to promote organic farming there. However, for some species during migration, increased heterogeneity in homogeneous landscapes may have negative effects. We propose that migratory bird diversity in homogeneous landscapes may be best preserved by keeping the landscape open, but that a reduced agricultural intensity, such as organic farming, should be encouraged. 相似文献
102.
103.
The airborne dispersal of the anamorphs of the Gibberella fujikuroi species complex was studied under pre- and postharvest maize (corn) production conditions using a 3-stage Andersen sampler.
The aim of this study was to identify and analyse the size distribution of such species in air samples. Differences were observed
between the concentration of large- and small-sized propagules (identified as aggregates and single microconidia, respectively),
but the difference was only significant during a high concentration period (October 2007, P = 0.009). No correlation was found between the concentration of fusaria found at different sampling heights (10 and 150 cm
above ground level). Fusarium isolates were collected and identified based on morphological characters and using species-specific PCR assays. The PCR analysis
confirmed morphological identification of F. verticillioides, F. proliferatum and F. subglutinans. High concentrations were found during the maize harvest, loading and corn shelling. Our results showed that the monitoring
of F. verticillioides should be performed at a single sampling height. 相似文献
104.
Viktória Jeney Edina Komódi Emőke Nagy Abolfazl Zarjou Gregory M. Vercellotti John W. Eaton György Balla József Balla 《Free radical biology & medicine》2009,46(5):616-623
Heme-mediated oxidative modification of low-density lipoprotein (LDL) plays a crucial role in early atherogenesis. It has been shown that hydrogen sulfide (H2S) produced by vascular smooth muscle cells is present in plasma at a concentration of about 50 µmol/L. H2S is a strong reductant which can react with reactive oxygen species like superoxide anion and hydrogen peroxide. The current study investigated the effect of H2S on hemin-mediated oxidation of LDL and oxidized LDL (oxLDL)-induced endothelial reactions. H2S dose dependently delayed the accumulation of lipid peroxidation products—conjugated dienes, lipid hydroperoxides (LOOH), and thiobarbituric acid reactive substances—during hemin-mediated oxidation. Moreover, H2S decreased the LOOH content of both oxidized LDL and lipid extracts derived from soft atherosclerotic plaque, which was accompanied by reduced cytotoxicity. OxLDL-mediated induction of the oxidative stress responsive gene, heme oxygenase-1, was also abolished by H2S. Finally we have shown that H2S can directly protect endothelium against hydrogen peroxide and oxLDL-mediated endothelial cytotoxicity. These results demonstrate novel functions of H2S in preventing hemin-mediated oxidative modification of LDL, and consequent deleterious effects, suggesting a possible antiatherogenic action of H2S. 相似文献
105.
106.
M. L. A. Haeck S. L. M. A. Beeres U. H?ke M. Palmen L. E. Couperus V. Delgado E. A. Logeman J. J. Maas R. J. M. Klautz M. J. Schalij H. F. Verwey 《Netherlands heart journal》2015,23(2):102-108
PurposeMechanical circulatory support with a continuous-flow left ventricular assist device (LVAD) may be a valuable treatment in end-stage heart failure patients for an extended period of time. The purpose of this study was to evaluate the safety and efficacy of implantation of a continuous-flow LVAD in end-stage heart failure patients within the first destination program in the Netherlands.MethodsA third-generation LVAD was implanted in 16 heart failure patients (age 61 ± 8; 81 % male; left ventricular ejection fraction 20 ± 6 %) as destination therapy. All patients were ineligible for heart transplant. At baseline, 3 and 6 months, New York Heart Association (NYHA) functional class, quality-of-life and exercise capacity were assessed. Clinical adverse events were registered.ResultsSurvival at 30 days and 6 months was 88 and 75 %, respectively. In the postoperative phase, 6 (38 %) patients required continuous veno-venous haemofiltration for renal failure and 2 (13 %) patients required extracorporeal membrane oxygenation because of severe right ventricular failure. During follow-up, NYHA functional class and quality-of-life improved from 3.7 ± 0.1 to 2.3 ± 0.1 and 57 ± 5 to 23 ± 3 at 6 months (P < 0.001), respectively. The 6 min walking distance improved from 168 ± 42 m to 291 ± 29 m at 6 months (P = 0.001).ConclusionContinuous-flow LVAD therapy is a promising treatment for patients with end-stage heart failure ineligible for heart transplant. 相似文献
107.
Overexpression of heat shock protein 27 (HSP27) increases gemcitabine sensitivity in pancreatic cancer cells through S‐phase arrest and apoptosis 下载免费PDF全文
Yang Guo Andreas Ziesch Sandra Hocke Eric Kampmann Stephanie Ochs Enrico N. De Toni Burkhard Göke Eike Gallmeier 《Journal of cellular and molecular medicine》2015,19(2):340-350
We previously established a role for HSP27 as a predictive marker for therapeutic response towards gemcitabine in pancreatic cancer. Here, we investigate the underlying mechanisms of HSP27‐mediated gemcitabine sensitivity. Utilizing a pancreatic cancer cell model with stable HSP27 overexpression, cell cycle arrest and apoptosis induction were analysed by flow cytometry, nuclear staining, immunoblotting and mitochondrial staining. Drug sensitivity studies were performed by proliferation assays. Hyperthermia was simulated using mild heat shock at 41.8°C. Upon gemcitabine treatment, HSP27‐overexpressing cells displayed an early S‐phase arrest subsequently followed by a strongly increased sub‐G1 fraction. Apoptosis was characterized by PARP‐, CASPASE 3‐, CASPASE 8‐, CASPASE 9‐ and BIM‐ activation along with a mitochondrial membrane potential loss. It was reversible through chemical caspase inhibition. Importantly, gemcitabine sensitivity and PARP cleavage were also elicited by heat shock‐induced HSP27 overexpression, although to a smaller extent, in a panel of pancreatic cancer cell lines. Finally, HSP27‐overexpressing pancreatic cancer cells displayed an increased sensitivity also towards death receptor‐targeting agents, suggesting another pro‐apoptotic role of HSP27 along the extrinsic apoptosis pathway. Taken together, in contrast to the well‐established anti‐apoptotic properties of HSP27 in cancer, our study reveals novel pro‐apoptotic functions of HSP27—mediated through both the intrinsic and the extrinsic apoptotic pathways—at least in pancreatic cancer cells. HSP27 could represent a predictive marker of therapeutic response towards specific drug classes in pancreatic cancer and provides a novel molecular rationale for current clinical trials applying the combination of gemcitabine with regional hyperthermia in pancreatic cancer patients. 相似文献
108.
109.
Marianne Danielsen Marius C. Codrea Klaus L. Ingvartsen Nicolas C. Friggens Emøke Bendixen Christine M. Røntved 《Proteomics》2010,10(12):2240-2249
Intramammary infusion of lipopolysaccharide (LPS) in cows induces udder inflammation that partly simulates mastitis caused by infection with Gram‐negative bacteria. We have used this animal model to characterize the quantitiative response in the milk proteome during the time course before and immediately after the LPS challenge. Milk samples from three healthy cows collected 3 h before the LPS challenge were compared with milk samples collected 4 and 7 h after the LPS challenge, making it possible to describe the inflammatory response of individual cows. Quantitative protein profiles were obtained for 80 milk proteins, of which 49 profiles changed significantly for the three cows during LPS challenge. New information obtained in this study includes the quantified increase of apolipoproteins and other anti‐inflammatory proteins in milk, which are important for the cow's ability to balance the immune response, and the upregulation of both complement C3 and C4 indicates that more than one complement pathway could be activated during LPS‐induced mastitis. In the future, this analytical approach may provide valuable information about the differences in the ability of individual cows to resist and recover from mastitis. 相似文献
110.
Martin Kellner Agnese Kolodinska Brantestam Inger Åhman Velemir Ninkovic 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(6):1133-1139
Recent studies have shown that volatile chemical interaction between certain barley (Hordeum vulgare) cultivars can cause reduced host plant acceptance by the aphid Rhopalosiphum padi, and that certain cultivars can induce this effect while others can respond. In this study, we tested whether inducing and
responding capabilities are linked to year of release in Swedish two-rowed spring barley. Eighteen cultivars released between
1897 and 1992 were tested in randomly selected subsets with pairwise combinations of volatile emitters and receivers. Significantly
reduced aphid acceptance as a result of exposure to volatiles from plants of a different cultivar were found in 24% of the
cultivar combinations. In general, older cultivars had a higher degree of aphid resistance after barley volatile treatment
than did younger cultivars. The inducing effect of the emitter was also related to date of emitter cultivar release but the
time relationship was reversed. Combinations with a younger volatile emitter and an older volatile receiver gave the strongest
reduction in aphid acceptance of treated plants. Linear relationships between microsatellite diversity of emitting cultivars
and their efficiency as inducers indicated that younger cultivars might have a more unique odour, whereas older cultivars
may be more sensitive to induction. 相似文献