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431.
Kakehasi FM Pinto JA Romanelli RM Carneiro M Cardoso CS Tavares Mdo C Melo VH Aguiar RA 《Memórias do Instituto Oswaldo Cruz》2008,103(4):351-357
Significant decrease in human immunodeficiency virus type 1 (HIV-1) vertical transmission has been observed worldwide in centers where interventions such as antiretroviral therapy (ART), elective cesarean section, and avoidance of breastfeeding have been implemented. This prospective cohort study aimed to assess the determinants of and the temporal trends in HIV-1 vertical transmission in the metropolitan area of Belo Horizonte, Brazil from January 1998 to December 2005. The rate of HIV-1 vertical transmission decreased from 20% in 1998 to 3% in 2005. This decline was associated with increased use of more complex ART regimens during pregnancy. Multivariate analysis restricted to clinical variables demonstrated that non ART, neonatal respiratory distress/sepsis and breastfeeding were independently associated with HIV-1 vertical transmission. When laboratory parameters were included in the model, high maternal viral load and non maternal ART were associated with HIV-1 vertical transmission. The results from this study confirm the impact of ART in the reduction of HIV-1 vertical transmission and indicate the need for improvement in the care and monitoring of mother and infant pairs affected by HIV-1. 相似文献
432.
Ademir Sergio Ferreira de Araujo Walderly Melgaço Bezerra Vilma Maria dos Santos Sandra Mara Barbosa Rocha Nilza da Silva Carvalho Maria do Carmo Catanho Pereira de Lyra Marcia do Vale Barreto Figueiredo Ângela Celis de Almeida Lopes Vania Maria Maciel Melo 《Antonie van Leeuwenhoek》2017,110(4):457-469
The Cerrado biome in the Sete Cidades National Park, an Ecological Reserve in Northeastern Brazil, has conserved its native biodiversity and presents a variety of plants found in other savannas in Brazil. Despite this finding the soil microbial diversity and community structure are poorly understood. Therefore, we described soil bacterial diversity and distribution along a savanna vegetation gradient taking into account the prevailing environmental factors. The bacterial composition was retrieved by sequencing a fragment of the 16S ribosomal RNA gene. The bacterial operational taxonomic units (OTUs) were assigned to 37 different phyla, 96 classes, and 83 genera. At the phylum level, a core comprised by Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes, Verrucomicrobia and Planctomycetes, was detected in all areas of Cerrado. ‘Cerrado stricto sensu’ and ‘Cerradao’ share more similarities between edaphic properties and vegetation and also present more similar bacterial communities, while ‘Floresta decidual’ and ‘Campo graminoide’ show the largest environmental differences and also more distinct bacterial communities. Proteobacteria (26%), Acidobacteria (21%) and Actinobacteria (21%) were the most abundant phyla within the four areas. All the samples present similar bacteria richness (alpha diversity) and the observed differences among them (beta diversity) were more related to the abundance of specific taxon OTUs compared to their presence or absence. Total organic C, N and P are the main abiotic factors structuring the bacterial communities. In summary, our findings show the bacterial community structure was clearly different across the Cerrado gradient, but that these environments share a bacterial phylum-core comprising Proteobacteria, Acidobacteria, Actinobacteria, Verrucomicrobia and Planctomycetes with other Brazilian savannas. 相似文献
433.
Interspecific variation in neighbour–stranger discrimination in ants of the Neoponera apicalis complex 下载免费PDF全文
BORIS YAGOUND MATHILDE CROWET CHLOÉ LEROY CHANTAL POTEAUX NICOLAS CH ÂLINE 《Ecological Entomology》2017,42(2):125-136
1. The ecological success of social insects lies in their ability to prevent the exploitation of colony resources by competitors or parasites. Nestmate recognition is therefore of crucial importance in maintaining the integrity of the colony. Furthermore, inter‐colony competitive relationships are often complex, as many species discriminate between neighbours and strangers, with reduced (the dear enemy phenomenon) or increased levels of aggression towards nearby colonies, depending on the species. In this context, between‐species comparisons could be particularly helpful to investigate the proximate causes underlying this context‐dependent phenomenon, but these are notoriously lacking. 2. Here an attempt was made to circumvent this drawback by studying three closely related sympatric ant species with very similar life histories that belong to the Neoponera apicalis complex. The present study investigated how nestmate recognition and inter‐colony competitive relationships were influenced by spatial, chemical and genetic distances between colonies. 3. It was found that one species, N. apicalis morph 7, showed a clear dear enemy phenomenon with no influence of chemical and genetic distances, suggesting the existence of a learning process. In contrast, N. apicalis morph 4 and Neoponera verenae morph 1 failed to show any strong discrimination between close and distant non‐nestmates. 4. These results are discussed in the light of the observed interspecific variation in nesting preferences, possibly constraining the opportunities of familiarisation between nearby nests, and modulating the competition for resources between colonies. 5. It is argued that this study further reinforces the relative threat level hypothesis as an ultimate explanation for neighbour–stranger discrimination processes. 相似文献
434.
Lamjed Mansour Carlos Azevedo Ângela Alves Saleh Al-Quraishy Abdel-Azeem S. Abdel-Baki 《Systematic parasitology》2017,94(1):123-131
A new myxosporean parasite, Auerbachia maamouni n. sp., infecting the gallbladder of the golden trevally Gnathanodon speciosus Forsskål, is described, based on morphology, ultrastructure, and small subunit (SSU) rRNA gene sequencing. Of 50 fish collected from the Red Sea in Jeddah, Saudi Arabia, five were found heavily infected with mature myxospores floating free in the bile. Mature spores are pyriform to club-shaped with smooth valves, and contain a single polar capsule with an S-shaped polar filament, arranged in 13–16 polar filament coils, oriented longitudinally, with an irregular distribution on the polar capsule matrix. Spores measure 15.8 (14–17) μm in total length in lateral view, 7.9 (6–9) μm in width in apical view, with spore body length of 6.2 ± 0.4 (5–7) µm. The ellipsoidal polar capsule has two adjusted lateral folds 7.6 (6–8) µm long and 2 (2–3) µm wide. The new species is distinguished from other species of the genus based on spore morphometry and molecular data. The phylogenetic tree constructed using Maximum Likelihood and Bayesian Inference analysis of SSU rDNA sequence data supported the phylogenetic position of A. maamouni n. sp. among the species of Auerbachia Meglitsch, 1968 sequenced to date. Analysis of the SSU rDNA sequence data also supported the assumption that Auerbachia is closely related to members of the genera Coccomyxa Léger & Hesse, 1907, Zschokkella Auerbach, 1910 and Myxidium Bütschli, 1882, whose members inhabit the gallbladder of marine teleost fishes. 相似文献
435.
436.
Glutaric Acid Administration Impairs Energy Metabolism
in Midbrain and Skeletal Muscle of Young Rats
Ferreira Gda C Viegas CM Schuck PF Tonin A Ribeiro CA Coelho Dde M Dalla-Costa T Latini A Wyse AT Wannmacher CM Vargas CR Wajner M 《Neurochemical research》2005,30(9):1123-1131
A genetic mice model of glutaric acidemia type I (GAI) has recently been developed, however affected animals do not develop
the striatal damage characteristic of patients with this disorder. Therefore, the initial aim of the present work was to induce
high glutaric acid (GA) concentrations in rat brain similar to those found in GAI patients through subcutaneous injection
of GA. High brain GA concentrations (up to 0.60 μmol/g ≅ 0.60mM) were achieved by a single subcutaneous injection of saline-buffered
GA (5 μmol/g body weight) to Wistar rats of 7–22 days of life. GA brain levels were about 10-fold lower than in plasma and
5-fold lower than in skeletal and cardiac muscles, indicating that the permeability of the blood brain barrier to GA is low.
We also aimed to use this model to investigate neurochemical parameters in the animals. Thus, we evaluated the effect of this
model on energy metabolism parameters in midbrain, in which the striatum is localized, as well as in peripheral tissues (skeletal
and cardiac muscles) of 22-day-old rats. Control rats were treated with saline in the same volumes. We verified that CO2 production from glucose was not altered in midbrain of rats treated with GA, indicating a normal functioning of the tricarboxylic
acid cycle. Creatine kinase activity was also not changed in midbrain, skeletal and cardiac muscles. In contrast, complex
I–III activity of the respiratory chain was inhibited in midbrain (25%), while complexes I–III (25%) and II–III (15%) activities
were reduced in skeletal muscle, with no alterations found in cardiac muscle. These data indicate that GA administration moderately
impairs cellular energy metabolism in midbrain and skeletal muscle of young rats. 相似文献
437.
438.
Marinho CR Barbosa LS de Azevedo AC Queiroz MM Valgode MA Coelho VM 《Memórias do Instituto Oswaldo Cruz》2003,98(7):937-938
The aim of this note was to record for the first time the finding of Hemilucilia segmentaria acting as biological vector of Dermatobia hominis, during a study of the diversity of Calliphoridae at Reserva Biol gica do Tinguá, Rio de Janeiro, Brazil. The insects were captured using traps baited with chicken vicera, for a period of 28-30 h twice per month. In the period of one year, 1987 insects were collected, 7.5% of which belonged to the H. segmentaria; of these a female was captured in May 2001, carrying a mass of 20 eggs on the left side of its abdomen. 相似文献
439.
Freire N Rodrigues-Silva R Machado-Silva JR Rey L 《Memórias do Instituto Oswaldo Cruz》2003,98(6):783-787
Experiments were carried out to analyze the biological characteristics of two sympatric isolates of Schistosoma mansoni derived from humans and murines in a low endemic transmission area (Sumidouro county, state of Rio de Janeiro, Brazil). Sympatric reared-laboratory Biomphalaria glabrata and C3H/He mice were used as experimental hosts. Parameters assessed comprised: precercarial period, infectivity and mortality (snails), prepatent period, infectivity (percentage of cercariae maturation into adult worm) and intestinal egg count (mice). The murine isolate showed a shorter precercarial period and higher infectivity than human isolate (p<0.05). This biological heterogenicity did not correspond to the vertebrate data because any biological parameter presented significant difference (p>0.05). These data suggest that both isolates are local sub-populations, providing support for the hypotheses that in a same biotope mixed populations or sub-populations circulate among their main host (human beings) and/or rodent as an anfixenous infection. 相似文献
440.
Assis GF Ceolin DS Marques ME Salvadori DM Ribeiro DA 《Journal of molecular histology》2005,36(8-9):483-489
While it has been clearly demonstrated that smoking is the most significant exogenous factor involved in oral carcinogenesis,
little is known about the global molecular and cellular changes that occur prior to the appearance of clinically detectable
symptoms. Thus, the aim of this study was to investigate the expressivity of bcl-2, bax and PCNA in the rat tongue mucosa
exposed to cigarette smoke by means of immunohistochemistry. A total of twelve male Wistar rats were distributed into 2 groups:
negative control and experimental group exposed to cigarette smoke during 75 days. After experimental period, no histopathological
changes in the tongue mucosa were detected in the negative control and the experimental group. On the other hand, an overexpression
of bcl-2 was detected (p < 0.01) throughout all layers of the epithelium, whereas bax did not show significant differences (p > 0.05). Also, the labeling index for bcl-2 and bax showed an increase 75 days after cigarette exposure (p < 0.01). PCNA-labeling index did not show remarkable changes between groups. Taken together, our results show that bcl-2
is overexpressed in the rat tongue keratinocytes after cigarette smoke exposure. 相似文献