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991.
Álvaro Fernández-Llamazares Jordina Belmonte Martí Boada Sara Fraixedas 《Aerobiologia》2014,30(2):111-122
The magnitude and complexity of the current erosion of plant biodiversity call for the development of interdisciplinary tools that enable an early detection of its effects and the establishment of effective management strategies. Indeed, plant sciences face the complex task of identifying the ecological information needed for the conservation challenge. Along this line should be placed the approach of aerobiology to gather the essential information for the development of plant recovery guidelines. In this work, we aim to discuss the potential role of airborne pollen monitoring in providing relevant data for the protection of plants and its potential applications to the management of plant diversity. To this end, we review three study cases where aerobiological monitoring can provide significant insights on conservation science. The present study is a contribution to plant conservation biology through long-term aeropalynological sampling, on the basis that pollen records constitute a suitable indicator for evaluating resource conservation of vegetation responding to environmental fluctuations. In view of its position between botany and meteorology, the contribution of aerobiological knowledge to biodiversity conservation can be very relevant and should be explored thoroughly. 相似文献
992.
993.
Juan Diego Ibáñez‐Álamo Magdalena Ruiz‐Rodríguez Juan José Soler 《Journal of avian biology》2014,45(4):354-358
Nestlings of many bird species produce fecal sacs, excrements encapsulated within a mucous covering. Although it facilitates parents' removal of feces from nests, which would improve hygienic conditions for developing nestlings, no functional (i.e. adaptive) explanation of fecal sac production has been previously investigated. We propose that the mucous covering would isolate enteric pathogenic bacteria, thereby preventing contamination of nestlings and parents. This antimicrobial hypothesis therefore predicts that density of bacteria would be drastically reduced from the inside to the outside of nestlings' droppings, and that the fecal sac covering would inhibit other bacterial grow. We tested these predictions by means of culturing bacteria obtained from different parts of the sac and inhibition tests. In accordance with the hypothesis, bacterial loads of the outside of fecal sacs were significantly lower than those estimated from the inside of the covering. In addition, we did not find evidence of antimicrobial activity of the covering, which suggests that the hypothesized bacterial isolation function is accomplished by a physical rather than a chemical protection. Bacterial density of the liquid that permeates out after 23 min does not differ with that estimated for the inside of the sac, suggesting short‐term effects of fecal sacs as bacterial barrier. These findings highlight the major role of bacterial infections as a selective pressure for explaining the evolution of traits that, as the covering of fecal sacs, facilitate nest sanitation in this group of animals. 相似文献
994.
Hervey Rodríguez-González Javier Orduña-Rojas José P. Villalobos-Medina Manuel García-Ulloa Arturo Polanco-Torres Ely S. López-Álvarez Magnolia Montoya-Mejía Alfredo Hernández-Llamas 《Journal of applied phycology》2014,26(6):2453-2459
The algae Ulva lactuca and Gracilaria parvispora are abundant in the Gulf of California, rich in nutrients, and may be used as a source of protein in balanced diets for shrimp. This study tests whether their meal, as a partial inclusion in diets for juvenile Litopenaeus vannamei, is feasible. Percentages of inclusion were 5, 10, and 15 %. Results showed that final weight, weight gain, and specific growth rate varied significantly among diets (P?0.05). There were significant differences in growth among the trials of amount of inclusion of meal when using U. lactuca (P?0.05), and no significant differences among the trials when using G. parvispora (P?>?0.05). In general, better results were obtained when using G. parvispora compared with U. lactuca. When compared to the control diet (without inclusion), diets that included 10 and 15 % U. lactuca meal yielded a significantly lower growth (P?0.05), but no significant differences were detected when using U. lactuca 5 % meal (P?>?0.05), suggesting the feasibility of inclusion to this limited percentage. No significant differences were detected between the control and the three treatments with G. parvispora, suggesting the possibility of using higher percentages of inclusion. We conclude that both seaweeds may be used as a component in preparing feed for juvenile L. vannamei. 相似文献
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997.
José C. Brito Raquel Godinho Fernando Martínez‐Freiría Juan M. Pleguezuelos Hugo Rebelo Xavier Santos Cândida G. Vale Guillermo Velo‐Antón Zbyszek Boratyński Sílvia B. Carvalho Sónia Ferreira Duarte V. Gonçalves Teresa L. Silva Pedro Tarroso João C. Campos João V. Leite Joana Nogueira Francisco Álvares Neftalí Sillero Andack S. Sow Soumia Fahd Pierre‐André Crochet Salvador Carranza 《Biological reviews of the Cambridge Philosophical Society》2014,89(1):215-231
Deserts and arid regions are generally perceived as bare and rather homogeneous areas of low diversity. The Sahara is the largest warm desert in the world and together with the arid Sahel displays high topographical and climatic heterogeneity, and has experienced recent and strong climatic oscillations that have greatly shifted biodiversity distribution and community composition. The large size, remoteness and long‐term political instability of the Sahara‐Sahel, have limited knowledge on its biodiversity. However, over the last decade, there have been an increasing number of published scientific studies based on modern geomatic and molecular tools, and broad sampling of taxa of these regions. This review tracks trends in knowledge about biodiversity patterns, processes and threats across the Sahara‐Sahel, and anticipates needs for biodiversity research and conservation. Recent studies are changing completely the perception of regional biodiversity patterns. Instead of relatively low species diversity with distribution covering most of the region, studies now suggest a high rate of endemism and larger number of species, with much narrower and fragmented ranges, frequently limited to micro‐hotspots of biodiversity. Molecular‐based studies are also unravelling cryptic diversity associated with mountains, which together with recent distribution atlases, allows identifying integrative biogeographic patterns in biodiversity distribution. Mapping of multivariate environmental variation (at 1 km × 1 km resolution) of the region illustrates main biogeographical features of the Sahara‐Sahel and supports recently hypothesised dispersal corridors and refugia. Micro‐scale water‐features present mostly in mountains have been associated with local biodiversity hotspots. However, the distribution of available data on vertebrates highlights current knowledge gaps that still apply to a large proportion of the Sahara‐Sahel. Current research is providing insights into key evolutionary and ecological processes, including causes and timing of radiation and divergence for multiple taxa, and associating the onset of the Sahara with diversification processes for low‐mobility vertebrates. Examples of phylogeographic patterns are showing the importance of allopatric speciation in the Sahara‐Sahel, and this review presents a synthetic overview of the most commonly hypothesised diversification mechanisms. Studies are also stressing that biodiversity is threatened by increasing human activities in the region, including overhunting and natural resources prospection, and in the future by predicted global warming. A representation of areas of conflict, landmines, and natural resources extraction illustrates how human activities and regional insecurity are hampering biodiversity research and conservation. Although there are still numerous knowledge gaps for the optimised conservation of biodiversity in the region, a set of research priorities is provided to identify the framework data needed to support regional conservation planning. 相似文献
998.
JAVIERABEL MENENDEZ MENENDEZ TOMÁS ALARCÓN BRUNA COROMINAS-FAJA ELISABET CUYÀS Eugeni López-Bonet ÁNGEL MARTÍN 《Cell cycle (Georgetown, Tex.)》2014,13(3):358-370
In the science-fiction thriller film Minority Report, a specialized police department called “PreCrime” apprehends criminals identified in advance based on foreknowledge provided by 3 genetically altered humans called “PreCogs”. We propose that Yamanaka stem cell technology can be similarly used to (epi)genetically reprogram tumor cells obtained directly from cancer patients and create self-evolving personalized translational platforms to foresee the evolutionary trajectory of individual tumors. This strategy yields a large stem cell population and captures the cancer genome of an affected individual, i.e., the PreCog-induced pluripotent stem (iPS) cancer cells, which are immediately available for experimental manipulation, including pharmacological screening for personalized “stemotoxic” cancer drugs. The PreCog-iPS cancer cells will re-differentiate upon orthotopic injection into the corresponding target tissues of immunodeficient mice (i.e., the PreCrime-iPS mouse avatars), and this in vivo model will run through specific cancer stages to directly explore their biological properties for drug screening, diagnosis, and personalized treatment in individual patients. The PreCog/PreCrime-iPS approach can perform sets of comparisons to directly observe changes in the cancer-iPS cell line vs. a normal iPS cell line derived from the same human genetic background. Genome editing of PreCog-iPS cells could create translational platforms to directly investigate the link between genomic expression changes and cellular malignization that is largely free from genetic and epigenetic noise and provide proof-of-principle evidence for cutting-edge “chromosome therapies” aimed against cancer aneuploidy. We might infer the epigenetic marks that correct the tumorigenic nature of the reprogrammed cancer cell population and normalize the malignant phenotype in vivo. Genetically engineered models of conditionally reprogrammable mice to transiently express the Yamanaka stemness factors following the activation of phenotypic copies of specific cancer diseases might crucially evaluate a “reprogramming cure” for cancer. A new era of xenopatients 2.0 generated via nuclear reprogramming of the epigenetic landscapes of patient-derived cancer genomes might revolutionize the current personalized translational platforms in cancer research. 相似文献
999.
Kinga Tóth Reinhold Wasserkort Ferenc Sipos Alexandra Kalmár Barnabás Wichmann Katalin Leiszter Gábor Valcz Márk Juhász Pál Miheller árpád V. Patai Zsolt Tulassay Béla Molnár 《PloS one》2014,9(12)
Background
Determination of methylated Septin 9 (mSEPT9) in plasma has been shown to be a sensitive and specific biomarker for colorectal cancer (CRC). However, the relationship between methylated DNA in plasma and colon tissue of the same subjects has not been reported.Methods
Plasma and matching biopsy samples were collected from 24 patients with no evidence of disease (NED), 26 patients with adenoma and 34 patients with CRC. Following bisulfite conversion of DNA a commercial RT-PCR assay was used to determine the total amount of DNA in each sample and the fraction of mSEPT9 DNA. The Septin-9 protein was assessed using immunohistochemistry.Results
The percent of methylated reference (PMR) values for SEPT9 above a PMR threshold of 1% were detected in 4.2% (1/24) of NED, 100% (26/26) of adenoma and 97.1% (33/34) of CRC tissues. PMR differences between NED vs. adenoma and NED vs. CRC comparisons were significant (p<0.001). In matching plasma samples using a PMR cut-off level of 0.01%, SEPT9 methylation was 8.3% (2/24) of NED, 30.8% (8/26) of adenoma and 88.2% (30/34) of CRC. Significant PMR differences were observed between NED vs. CRC (p<0.01) and adenoma vs. CRC (p<0.01). Significant differences (p<0.01) were found in the amount of cfDNA (circulating cell-free DNA) between NED and CRC, and a modest correlation was observed between mSEPT9 concentration and cfDNA of cancer (R2 = 0.48). The level of Septin-9 protein in tissues was inversely correlated to mSEPT9 levels with abundant expression in normals, and diminished expression in adenomas and tumors.Conclusions
Methylated SEPT9 was detected in all tissue samples. In plasma samples, elevated mSEPT9 values were detected in CRC, but not in adenomas. Tissue levels of mSEPT9 alone are not sufficient to predict mSEPT9 levels in plasma. Additional parameters including the amount of cfDNA in plasma appear to also play a role. 相似文献1000.