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11.
Dr. Ambrosius Ábrahám 《Cell and tissue research》1929,9(4):694-696
Ohne Zusammenfassung
Mit 2 Textabbildungen 相似文献
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A M O'Brien C O'Fágáin P F Nielsen K G Welinder 《Biotechnology and bioengineering》2001,76(4):277-284
The bifunctional compound, ethylene-glycol bis(N-hydroxysuccinimidylsuccinate) (EGNHS), stabilizes horseradish peroxidase C (HRP) by reaction with the enzyme's lysine residues. In this study we compare native and modified HRP by proteolytic fragmentation, peptide sequencing, and mass spectroscopy, and identify the sites of modification. Most significantly, EGNHS is shown to form a crosslink between Lys232 and Lys241 of HRP and modifies Lys174 without formation of a crosslink. These findings are in agreement with the lysine side-chain reactivities predicted from the surface accessibility of the amino groups, and the maximal span of 16 A of the EGNHS crosslinker. 相似文献
16.
S Rogne G Skretting F Larsen O Myklebost B Mev?g L A Carlson L Holmquist E Gjone H Prydz 《Biochemical and biophysical research communications》1987,148(1):161-169
We have isolated cDNA clones coding for human lecithin:cholesterol acyl transferase (LCAT) from a liver-specific cDNA library by the use of two oligonucleotide probes based on the protein sequence. The clones span the sequence coding for the entire secreted LCAT, the 3' untranslated sequence and 12 amino acids of the signal peptide. The peptide sequence contains the conserved active site of serine lipases within a hydrophobic domain, flanked by a possible amphipatic alpha-helix. Only one gene for LCAT could be detected in genomic blots. We have used the cDNA as a probe to analyse the LCAT gene in patients suffering from LCAT deficiency and fish eye disease. No rearrangements or abnormal gene fragments were detected in these patients. 相似文献
17.
Gabriela VizÁrovÁ 《Biologia Plantarum》1987,29(3):230-233
The cultivars of barley and wheat resistant to mildew had a higher level of free zeatin and its derivatives during the whole
ontogeny than the susceptible cultivars. The effect of exogenous application of cytokinins on the growth ofErysiphe graminis DC. was different. Kinetin and benzylaminopurine showed only a slight inhibitory effect. Zeatin and its derivatives completely
inhibited growth of this fungus.
Presented at the International Symposium “Plant Growth Regulators” held on June 18-22 1984 at Liblice, Czechoslovakia. 相似文献
18.
F Brégégère H Bedouelle 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1992,314(12):527-532
We have fused the variable domains of a mouse antibody to the C-terminal end of the maltose-binding protein (malE), at the genetic level. The hybrid proteins were expressed in E. coli under control of the malEp promoter, and exported to the periplasm, at low temperature. They were purified by affinity chromatography on cross-linked amylose. When the two variable domains were fused together through a peptide link, the hybrid displayed similar affinity and specificity to the antigen as the native antibody. 相似文献
19.
Sighvatur S. Árnason Erik Skadhauge 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1991,161(1):1-14
Summary The plasma levels of four osmoregulatory hormones and their target ion-transport systems in the lower intestines of the domestic fowl were determined in order to elucidate their interrelationship and their setpoints in relation to NaCl intake. White Plymouth Rock hens were adapted to six intake levels of NaCl (0.20±0.02–24.7±1.9 mmoles Na+·kg bw–1·day–1) for 6 weeks. The Na+ absorption and the Cl– secretion of colon and coprodeum were characterized in vitro by the effects of hexoses, amino acids, amiloride, and theophylline on the short-circuit current (SCC) and electrical potential difference (PD). The NaCl-conserving system of the adult chicken is set at low intake levels of NaCl as the 80% range (quantitized by non-linear, logistic regression analyses) of the change in the plasma [ALDO], the amiloride-inhibitable Na+ absorption of coprodeum and colon ( SCC), occurred from 0.18 to 2.3, from 0.9 to 4.3, and from 1.2 to 7.3 mmoles Na+·kg bw–1·day–1, respectively. These results demonstrate that the amiloride-inhibitable Na+ absorption of coprodcum is more closely linked to plasma [ALDO] than that of colon. The aminoacid-Na+ coabsorption of colon increased over exactly the same range of Na+ intake as the colonic amiloride-inhibitable Na+ absorption decreased, whereas the hexose-Na+ coabsorption increased at higher levels of Na+ intake, from 2 to 11 mmoles Na+·kg bw–1·day–1. Both these Na+ absorption types had reached their maximums at 24.7 mmoles Na+·kg bw–1·day–1, whereas the plasma [AVT] and plasma [PRL], although significantly increased, apparently had not; their 80% range of change occurred from 9.9 to 99 mmoles Na+·kg bw–1·day–1, and the main changes in plasma osmolity were predicted to occur from 5.4 to 107 mmoles Na+·kg bw–1·day–1. These results suggest that these colonic and hormonal variables conserve osmotically-free water and operate at high NaCl intake. The theophylline-induced colonic Cl– secretion did not change with NaCl intake, whereas the stimulation of SCC in coprodeum decreased with increasing NaCl intake: The main change occurred between 0 and 3.2 mmoles Na+·kg bw–1·day–1. Thus, all ion-transport capacity disappears in coprodeum with increased dietary NaCl intake, whereas colon maintains its ion-transport capacity (although the nature of the Na+ transport changes). It is suggested that hormones defending the extracellular volume and composition are regulated close to zero input and output of both NaCl and water, regardless of whether they are NaCl conserving or free-water conserving. Therefore, changes in their stable plasma concentrations occur at the extremes of tolerable range of NaCl intake.Abbreviation
AA
aminoacids
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ALDO
aldosterone
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AMI
amiloride
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AVT
arginine vasotocin
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bw
body weight
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CS
corticosterone
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HEX
hexoses
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INDO
indomethacin
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PD
potential difference
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PRL
prolactin
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R
resistance
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SCC
short-circuit current
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SD
standard deviation
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SEM
standard error of mean
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THEO
theophylline 相似文献
20.
A quantitative method for investigation of relationship between polygenic and monogenic traits has been proposed. It is based on examination of relationship between frequencies of distribution classes of an adaptive quantitative trait and frequencies of certain genetic character in the same classes. The method permits to locate a gene marker within a space of quantitative trait values. Using adaptively significant morpho-anatomic traits, it is possible to estimate indirectly adaptive values of gene markers under consideration, since, in accordance with the concept of adaptive norm, "average" phenotypes have maximal fitness, whereas deviative phenotypes transgress the bounds of the optimum. As a genetical character, genotype of certain biochemical locus, individual heterozygosity range or interlocus combinations of alleles could be used. The method has been applied to newborn Astrakhan lambs. Principal component analysis has been used to obtain complex characterization for six constitutional characters. Some regularities in location of homo- and heterozygous genotypes of the transferrin locus within a space of morphological characters' values have been revealed. 相似文献