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Characterisation and transferability of apple SSRs to two European pear F<Subscript>1</Subscript> populations 总被引:1,自引:0,他引:1
Pierantoni L Cho KH Shin IS Chiodini R Tartarini S Dondini L Kang SJ Sansavini S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(7):1519-1524
European pear (Pyrus communis L.) is among the important fruit species for which only few genetic studies have been carried out. Available evidence indicates that simple sequence repeats (SSR) are very useful as molecular markers because they are codominant, highly polymorphic, abundant and reproducible. The present paper reports more than 100 apple SSR markers in two populations of European pear; a total of 41 SSR markers were then positioned on a genetic linkage map of the cross Passe Crassane × Harrow Sweet and 31 in the map Abbè Fétel × Max Red Bartlett. Syntenic relationships between pear and apple maps have been considered for the chromosomes carrying two or more SSR markers. The alignment among the two maps supports the colinearity of the two genomes with respect both to identification and to orientation of the linkage groups. 相似文献
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根据2019—2021年在闽南渔场进行的秋季、冬季、春季和夏季四个航次定点底拖网调查资料,利用相对重要性指数、种群聚集强度、生态位宽度、生态位重叠及冗余分析对主要游泳动物时空生态位特征及其影响因素进行研究。结果表明,(1)调查海域共鉴定出游泳动物214种,主要优势种有18种,优势种存在明显的季节更替现象;夏季优势种的丛生指数和平均拥挤度较高,春季较低;(2)在时间维度上,须赤虾(Metapenaeposis barbata De Haan)生态位宽度最大(0.99), 7组种对时间生态位重叠值等于1.00;在空间维度上,带鱼(Trachurus japonicus Temminck&Schlegel)生态位宽度最大(2.57),空间生态位重叠值超过0.6的种类占71.3%;在时空维度上,带鱼生态位宽度最大(2.45),鹿斑仰口鲾(Leiognathus ruconius Hamilton)与赤鼻棱鳀(Thrissa kammalensis Bleeker)时空生态位重叠值最大(0.94);(3)冗余分析表明,底层温度和底层盐度是影响闽南渔场主要游泳动物时空生态位特征的重要环境因... 相似文献
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Phytochromes are molecular light switches by virtue of their photochromic red/far-red reversibility. The His-324 residue next to the chromophore-linked Cys-323 plays a critical role in conferring photochromism to the tetrapyrrole chromophore in native phytochrome A. The chromophore appears to be enclosed between the amphiphilic α-helical chains in a hydrophobic pocket. The absorbance maxima of both the Pr and the Pfr forms of pea phytochrome A are blue-shifted by 10 and 20 nm, respectively, upon C-terminal truncation. We speculate that the quaternary structure of the phytochrome A molecule involves some interactions of the C-terminal half with the chromophore domain. The Pfr conformation of phytochrome includes an amphiphilic α-helix of the amino terminal chain, which occurs in 113 ms after picosecond photoisomerization of the Pr form. Compared to α-helical folding, unfolding of the α-helix occurs faster in about 310 μs upon phototransformation of the Pfr form of phytochrome A. The photochromic transformation of phytochrome A modulates protein kinase-catalysed phosphorylation sites in vivo and in vitro, but only a subtle local change in conformation is detectable in the phosphorylated phytochromes. This suggests that the post-translational modification serves as a surface label, rather than a transducer-activating trigger, for the recognition of a putative phytochrome receptor. 相似文献
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Two different morphs of the small cryptomonad, Plagioselmis prolonga var. nordica with a posterior tail, were observed during summer and fall in the hypertrophic lake, Lake Kasumigaura, Japan. The tail shortened in mesocosms stocked with planktivorous silver carp ( Hypophthalmichthys molitrix Val.) and elongated by more than 50% in mesocosms from which silver carp were removed. The density of Plagioselmis cells increased significantly upon fish stocking and decreased upon fish removal. The tail length was negatively correlated with algal abundance and positively correlated with crustacean densities, but there was no correlation with nutrient levels or physical environmental parameters in the mesocosms. The variation in tail length was induced by the presence/absence of fish, but was not related to their density. However, silver carp manipulation strongly affected the density of the majority of zooplankters and, interestingly, there was a strong correlation between zooplankton density and tail-length change in Plagioselmis. We propose a possible herbivore-induced defense mechanism triggered by the top predator, silver carp. 相似文献
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Liu XM Fritz AK Reese JC Wilde GE Gill BS Chen MS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(8):1473-1480
H9, H10, and H11 are major dominant resistance genes in wheat, expressing antibiosis against Hessian fly [(Hf) Mayetiola destructor (Say)] larvae. Previously, H9 and H10 were assigned to chromosome 5A and H11 to 1A. The objectives of this study were to identify simple-sequence-repeat (SSR) markers for fine mapping of these genes and for marker-assisted selection in wheat breeding. Contrary to previous results, H9 and H10 did not show linkage with SSR markers on chromosome 5A. Instead, H9, H10, and H11 are linked with SSR markers on the short arm of chromosome 1A. Both H9 and H10 are tightly linked to flanking markers Xbarc263 and Xcfa2153 within a genetic distance of 0.3–0.5 cM. H11 is tightly linked to flanking markers Xcfa2153 and Xbarc263 at genetic distances of 0.3 cM and 1.7 cM. Deletion bin mapping assigned these markers and genes to the distal 14% of chromosome arm 1AS, where another Hf-resistance gene, Hdic (derived from emmer wheat), was also mapped previously. Marker polymorphism results indicated that a small terminal segment of chromosome 1AS containing H9 or H10 was transferred from the donor parent to the wheat lines Iris or Joy, and a small intercalary fragment carrying H11 was transferred from the resistant donor to the wheat line Karen. Our results suggest that H9, H10, H11, Hdic, and the previously identified H9- or H11-linked genes (H3, H5, H6, H12, H14, H15, H16, H17, H19, H28, and H29) may compose a cluster (or family) of Hf-resistance genes in the distal gene-rich region of wheat chromosome 1AS; and H10 most likely is the same gene as H9.Mention of commercial or proprietary product does not constitute an endorsement by the USDA. 相似文献
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Alcyonacean octocorals in tropical reefs are usually not considered as reef builders. Some Sinularia species, however, are capable of consolidating sclerites at the colony base to form spiculite. Nanwan Bay, southern Taiwan,
features both fossilized and recently formed boulders composed of spiculite, thus demonstrating the role of Sinularia in contributing to the reef structure. Section radiography of an 18.5 kg spiculite boulder demonstrated a regular density
banding of 3–6-mm intervals. Core survey indicated spiculite coverage of 25–30% on the live reef and of 30–40% on the uplifted
boulders. Cores taken from living Sinularia revealed a distinct transition from discrete sclerites to compact spiculite and amorphous calcium carbonate cementing the
sclerites. In the widespread S. gibberosa, sclerite formation appeared to start intracellularly, followed by a prolonged extracellular calcification process. At the
calcification site, multiple sclerocytes formed expanded pseudopod-like membranes that interconnected, forming multicellular
vesicles (MCVs) around the sclerites. The MCVs and the pseudopods disappeared at sclerite maturation, followed by degradation
of the sclerocytes around the mature sclerites. At the colony base, granular vesicles were distributed among the sclerites,
indicating a cementing process in progress. These findings suggest that colonies of Sinularia are able to cement sclerites and consolidate them at their base into spiculite, thus making them reef builders. 相似文献