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1.
Summary
E.
coli cells harbouring plasmid pBR322 which confers ampicillin resistance were immobilized on cotton cloth. The resulting film was used as an inoculum in daily repeated batch culture in ampicillin-free medium. During two months, the film was able to produce cultures which, at the late log phase, showed little sensitivity to 10 mg/ml ampicillin. Thus such a bacterial film can effectively be used as an inoculum for the production of recombinant DNA products by means of pBR322 or its derivatives in the absence of ampicillin. 相似文献
2.
Sulabha S. Keskar Sushama M. Gaikwad M.Islam Khan 《Enzyme and microbial technology》1996,18(8):602-604
Microbial α-mannosidases are used in the analysis of glycopeptides and the developmental regulation of lysosomal enzymes. This survey presents comparison of properties of this high molecular weight, oligomeric protein from a number of microbial sources. 相似文献
3.
Sushama Chaphalkar Rajdeep Dongre Deepa Joshi Sabita Dey 《Biotechnic & histochemistry》1993,68(3):166-168
A synthetic aromatic polymer has been used for preparing replicas of different microorganisms. This method of preparing highly concentrated (9.6 k) microbiological samples for scanning electron microscopy was compared with a standard method. The micrographs of the replicated samples are satisfactory. This method is rapid, cost effective and produces good results, especially in the case of spore-forming mycelial microorganisms. 相似文献
4.
Epichlorohydrin-triethanolamine (ECTEOLA)-cellulose films (paper and cloth) have been found to bind Saccharomyces cerevisiae cells which were able to develop metabolically active colonies on the surface of the films. Unmodified cellulose films also bound the yeast but to a lesser extent. Film fermenters were constructed by coiling a double layer of the cloth and copper screen and vertically placing the resulting cartridge into a column. These film fermenters were able to convert the sugars (14%) in the hydrolysate of a Jerusalem artichoke tuber into ethanol, with 90% of the theoretical yield after 6 h of fermentation. The bound yeast produced ethanol at a specific rate of 1.0 g ethanol per g cell per hour. 相似文献
5.
Steven A. Yukl Shahzada Khan Tsui-Hua Chen Martin Trapecar Frank Wu Guorui Xie Sushama Telwatte Daniel Fulop Alexander R. Pico Gregory M. Laird Kristen D. Ritter Norman G. Jones Chuanyi M. Lu Robert F. Siliciano Nadia R. Roan Jeffrey M. Milush Ma Somsouk Steven G. Deeks Peter W. Hunt Shomyseh Sanjabi 《Journal of virology》2021,95(2)
6.
Manasi Mishra Rakesh S. Joshi Sushama Gaikwad Vidya S. Gupta Ashok P. Giri 《Biochemical and biophysical research communications》2013,430(3):1060-1065
Pin-II protease inhibitors (PIs) are the focus of research interest because of their large structural–functional diversity and relevance in plant defense. Two representative Capsicum annuum PI genes (CanPI-15 and -7) comprising one and four inhibitory repeat domains, respectively, were expressed and recombinant proteins were characterized. β-Sheet and unordered structure was found predominant in CanPI-15 while -7 also displayed the signatures of polyproline fold, as revealed by circular dichroism studies. Inhibition kinetics against bovine trypsin indicated three times higher potency of CanPI-7 (Ki ~ 57 μM) than -15 (~184 μM). Activity and structural stability of these CanPIs were revealed under various conditions of pH, temperature and denaturing agent. Structure prediction, docking studies with proteases and mass spectroscopy revealed the organization of multiple reactive site loops of multi domain PIs in space as well as the steric hindrances imposed while binding to proteases due to their close proximity. 相似文献
7.
Expression of progesterone receptor (PR) localization on spermatozoa was determined in men with normal and abnormal spermiograms. Studies were also carried out to evaluate the potential of PR as a marker of sperm function. Progesterone receptor expression on spermatozoa from men with normozoospermia (n = 8), oligozoospermia (n = 7), asthenozoospermia (n = 8), oligoasthenozoospermia (n = 7), and teratozoospermia (n = 11) was analyzed using an immunocytochemical method with monoclonal antibodies against PR, and flow cytometry using a cell-impermeable fluorescein-tagged progesterone coupled to BSA complex (P-FITC-BSA). Both methods revealed significantly fewer (P < 0.05) PR-positive spermatozoa in men with oligozoospermia, asthenozoospermia, oligoasthenozoospermia, and teratozoospermia compared with men with normozoospermia, thereby suggesting that down-regulation of PR expression in spermatozoa may be one of the causes of male infertility. Spermatozoa from men with normozoospermia (n = 12), oligozoospermia (n = 12), asthenozoospermia (n = 12), oligoasthenozoospermia (n = 9), and teratozoospermia (n = 10) were exposed to low osmotic conditions in the hypoosmotic swelling (HOS) test and then analyzed for PR expression using P-FITC-BSA complex. A significantly higher percentage (P < 0.05) of spermatozoa with physiologically active plasma membrane (HOS+) lacked PR expression (HOS+PR-) in all categories of men with infertility, thereby suggesting that compared to the HOS test, PR expression is a better indicator of sperm function. Furthermore, PR expression in spermatozoa showed a strong (P < 0.05) positive correlation with their ability to undergo an in vitro acrosome reaction. This was observed in all study groups (i.e., normozoospermia, r = 0.8545; oligozoospermia, r = 0.8711; asthenozoospermia, r = 0.7645; oligoasthenozoospermia, r = 0.9003; and teratozoospermia, r = 0.8676). This suggests a potential role for PR in the events leading to the acrosome reaction in sperm. 相似文献
8.
9.
K.S. Shashidhara Sushama M. Gaikwad 《International journal of biological macromolecules》2009,44(1):112-115
Energetics of the catalysis of Class II α-mannosidase (E.C.3.2.1.24) from Aspergillus fischeri was studied. The enzyme showed Kcat/Km for Man (α1-3) Man, Man (α1-2) Man and Man (α1-6) Man as 7488, 5376 and 3690 M?1 min?1, respectively. The activation energy, Ea was 15.14, 47.43 and 71.21 kJ/mol for α1-3, α1-2 and α1-6 linked mannobioses, respectively, reflecting the energy barrier in the hydrolysis of latter two substrates. The enzyme showed Kcat/Km as 3.56 × 105 and 4.61 × 105 M?1 min?1 and Ea as 38.7 and 8.92 kJ/mol, towards pNPαMan and 4-MeUmbαMan, respectively. Binding of Swainsonine to the enzyme is stronger than that of 1-deoxymannojirimycin. 相似文献
10.
Sushama S. Gomare Dhawal P. Tamboli Anuradha N. Kagalkar Sanjay P. Govindwar 《International biodeterioration & biodegradation》2009,63(5):582-586
Brevibacillus laterosporus MTCC 2298 showed 87% decolorization of Golden Yellow HER within 48 h under static condition at the concentration 50 mg l?1; however no significant change in the decolorization performance was observed under shaking condition. Decolorization performance was maximum (74%) at the pH 7.0 and 30 °C. TLC and HPLC analysis confirmed the biodegradation of Golden Yellow HER. Biodegradation pathway was proposed using GC–MS and FTIR spectral analysis. Mainly elected metabolites are the 2,5-Dichloro-4 (3-hydrazino-2-hydroxy cyclopentylamino-) dibenzene-sulfonic acid (peak 1, m/z = 526), 4-(3-hydrazino-2-hydroxy cyclopentylamino)-benzene-sulfonic acid (peak 2, m/z = 455), 4-(3-amino-2-hydroxy-cyclopentylamino)-benzene-sulfonic acid and 5-amino-cyclohex-3-ene-sulfonic acid (peak 3, m/z = 183). Phytotoxicity results suggested that degradation products of Golden Yellow HER are non-toxic to the common crops such as Sorghum vulgare and Phaseolus mungo. Also, degradation products are non-toxic to B. laterosporus as well as ecologically important bacteria like Pseudomonas aeruginosa and Azotobacter vinelandii. 相似文献