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1.
Sønstebø JH Gielly L Brysting AK Elven R Edwards M Haile J Willerslev E Coissac E Rioux D Sannier J Taberlet P Brochmann C 《Molecular ecology resources》2010,10(6):1009-1018
Palaeoenvironments and former climates are typically inferred from pollen and macrofossil records. This approach is time-consuming and suffers from low taxonomic resolution and biased taxon sampling. Here, we test an alternative DNA-based approach utilizing the P6 loop in the chloroplast trnL (UAA) intron; a short (13–158 bp) and variable region with highly conserved flanking sequences. For taxonomic reference, a whole trnL intron sequence database was constructed from recently collected material of 842 species, representing all widespread and/or ecologically important taxa of the species-poor arctic flora. The P6 loop alone allowed identification of all families, most genera (>75%) and one-third of the species, thus providing much higher taxonomic resolution than pollen records. The suitability of the P6 loop for analysis of samples containing degraded ancient DNA from a mixture of species is demonstrated by high-throughput parallel pyrosequencing of permafrost-preserved DNA and reconstruction of two plant communities from the last glacial period. Our approach opens new possibilities for DNA-based assessment of ancient as well as modern biodiversity of many groups of organisms using environmental samples. 相似文献
2.
P R Carle J Coz N Elissa M Gasquet C Sannier A Richard P Timon-David 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1986,303(13):565-568
In vitro tests, deltamethrin reduced the parasitemic index of Plasmodium falciparum on human red blood corpuscles. In vivo tests, at sublethal doses, deltamethrin limited the development of the sporogonic cycle of Plasmodium yoelii yoelii in Anopheles stephensi. This novel type of antiplasmodic activity involve an intravectorial route and gives an explanation about surprising antimalaria effects observed several months after treatment. 相似文献
3.
Isabelle Garreau Ingrid Fruitier Frederic Sannier Qiuyu Zhao Karine Cucumel Anny Cupo Jean-Marie Piot 《Letters in Peptide Science》1997,4(4-6):293-296
Morphinomimetic peptides have been purified fromhemoglobin enzymatic hydrolysates and a significantamount of evidence has been accumulated indicatingthat the generation of these peptides (hemorphins)might occur in vivo. In order to investigatetheir putative physiological role and processing fromhemoglobin in vivo, two methods were developed:a specific radioimmunoassay and a UV spectracomparison analysis. These methods were applied to acathepsin D bovine hemoglobin hydrolysate and allowedthe detection of two hemorphin-7 peptides. Thisobservation supports the putative implication ofcathepsin D in the in vivo release ofhemorphins. Among the two methods used in this study,the immunological approach exhibits highersensitivity and represents a useful method toinvestigate the in vivo role and physiologicalprocessing of hemorphins. 相似文献
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Summary. Although the pollen grains produced in monocots are predominantly monosulcate (or monoporate), other aperture types are also
found within this taxonomic group, such as the trichotomosulcate, inaperturate, zonaperturate, di-, or triaperturate types.
The aperture pattern is determined during the young-tetrad stage of pollen development and it is known that some features
of microsporogenesis can constrain the aperture type. For example, trichotomosulcate pollen is always associated with simultaneous
cytokinesis, a condition considered as derived in the monocots. Our observations of the microsporogenesis pathway in a range
of monocot species show that this pathway is surprisingly variable. Our results, however preliminary, reveal that variation
in microsporogenesis concerns not only cytokinesis but also callose deposition among the microspores and shape of the tetrads.
The role played by these features in aperture pattern determination is discussed.
Correspondence and reprints: Laboratoire Ecologie, Systématique et Evolution, Université Paris-Sud, 91405 Orsay Cedex, France. 相似文献
6.
Thomas Loyau Sonia Métayer-Coustard Cécile Berri Sabine Crochet Estelle Cailleau-Audouin Mélanie Sannier Pascal Chartrin Christophe Praud Christelle Hennequet-Antier Nicole Rideau Nathalie Couroussé Sandrine Mignon-Grasteau Nadia Everaert Michel Jacques Duclos Shlomo Yahav Sophie Tesseraud Anne Collin 《PloS one》2014,9(9)
7.
Bovine whey hydrolysate has been developed and applied to areas such as nutrition, culture media, and isolation of bioactive peptides. In order to produce such a type of hydrolysate, it is possible to use goat whey, which constitutes also a food processing by-product. Enzymatic hydrolysis of goat whey by pepsin was carried out in a continuous ultrafiltration reactor. The permeate contained peptide hydrolysate that was resolved by RPHPLC. Second order derivative spectroscopy, amino acid analysis, and mass spectrometry revealed the presence of a biologically active peptide called alpha-lactorphin. This constitutes preliminary information about goat whey enzymatic degradation for future applications. 相似文献
8.
Efficiency and selectivity of 30 and 150 kd inorganic ultrafiltration membranes (Techsep) toward tuna hemoglobin and myoglobin were studied. The influence of pH and ionic strength was investigated. Mass flow of myoglobin was higher at its isoelectric pH (8.6) and for low ionic strength (1.5 mM). This result was related to the absence of electrostatic repulsion between myoglobin and the surface of the dynamic membrane. The use of high ionic strength 0.15 M NaCl involved an apparent dimerisation of myoglobin and consequently a lower permeation through the membrane due to the molecular weight increase. The permeation and retention of hemoglobin did not agree with the effect of pH observed with myoglobin (best permeation at isoelectric pH) but followed the behavior of myoglobin. This was explained by a myoglobin concentration 10 times higher than hemoglobin concentration. The yield of retention selectivity was investigated. Selectivity of the membrane at pH 8.6 and 1.5 mM was favorable to myoglobin (increase of 40%) whereas a reversed selectivity was observed at pH 7.3, 0.15 M. (c) 1996 John Wiley & Sons, Inc. 相似文献
9.
HPLC preparation of fish waste hydrolysate fractions. Effect on guinea pig ileum and ACE activity 总被引:3,自引:0,他引:3
Bordenave S Fruitier I Ballandier I Sannier F Gildberg A Batista I Piot JM 《Preparative biochemistry & biotechnology》2002,32(1):65-77
The effect of RP-HPLC-purified fractions of fish waste hydrolysates issued from three fish industries was tested on guinea pig ileum in order to examine the presence of opioid molecules. The evaluation of anti-hypertensive activities of whole hydrolysates and fractions were also tested, monitoring the ability of the fraction to inhibit the activity of angiotensin I-converting enzyme involved in hypertension regulation. Sardine autolysate and cod head hydrolysate powder (50 microg) were able to inhibit near 30% of ACE activity, whereas 50 microg of shrimp hydrolysate allows the inhibition of 57% of ACE activity. HPLC fractionation of cod head hydrolysate and sardine autolysate was necessary to evidence biological activity, whereas HPLC separation of shrimp hydrolysate exhibited low biological activity fractions. Further studies are necessary to characterise bioactive molecules from cod head alcalase hydrolysate and from sardine autolysate. 相似文献
10.
Florian Jabbour Guillaume Cossard Martine Le Guilloux Julie Sannier Sophie Nadot Catherine Damerval 《PloS one》2014,9(4)
Floral bilateral symmetry (zygomorphy) has evolved several times independently in angiosperms from radially symmetrical (actinomorphic) ancestral states. Homologs of the Antirrhinum majus Cycloidea gene (Cyc) have been shown to control floral symmetry in diverse groups in core eudicots. In the basal eudicot family Ranunculaceae, there is a single evolutionary transition from actinomorphy to zygomorphy in the stem lineage of the tribe Delphinieae. We characterized Cyc homologs in 18 genera of Ranunculaceae, including the four genera of Delphinieae, in a sampling that represents the floral morphological diversity of this tribe, and reconstructed the evolutionary history of this gene family in Ranunculaceae. Within each of the two RanaCyL (Ranunculaceae Cycloidea-like) lineages previously identified, an additional duplication possibly predating the emergence of the Delphinieae was found, resulting in up to four gene copies in zygomorphic species. Expression analyses indicate that the RanaCyL paralogs are expressed early in floral buds and that the duration of their expression varies between species and paralog class. At most one RanaCyL paralog was expressed during the late stages of floral development in the actinomorphic species studied whereas all paralogs from the zygomorphic species were expressed, composing a species-specific identity code for perianth organs. The contrasted asymmetric patterns of expression observed in the two zygomorphic species is discussed in relation to their distinct perianth architecture. 相似文献