我国高校人力资源开发的问题及其对策

分析了当前我国高校人力资源开发的重要性和存在的主要问题,并运用现代管理理论,提出了树立以人为本的管理理念、建立有利于人才成长的机制、运用柔性化管理模式的措施来促进我国高校人力资源的进一步开发利用。     

浙江晚稻稻种非致病细菌多样性初步研究

为了解特定生态环境中晚稻稻种的非致病细菌多样性以更有效地治理水稻主要病害,于1996－2001年间对采自浙江平原、丘陵及山区的606份稻种样本进行了非致病细菌种类、频率及对水稻纹枯病和恶苗病的抑菌作用研究。从中分离出9605个菌株,经致病性测定、菌落形态及部分细菌学特征（革兰氏染色、KMB培养基上的荧光色素和扩散性非荧光色素、YDC培养基上黄色菌落的产生、好气性、鞭毛及芽孢的染色镜检等）测定后,选出代表菌株622个,连同80个对照菌株用Biolog及脂肪酸分析法（FAME）进行测试。鉴定出Pseudomonas属11个种或型及其他14属的23种非致病细菌,并发现Pseudomonas属中55％的种及其他属中49％的种存在对水稻纹枯病或恶苗病的拮抗菌株,但不同种间的抑菌率存在较大差异。3类稻区均以Bacillus spp.、Acinetobacter spp.和P.putida等3个种群为主导并同时存在许多其他相似种,其中7个常见种的分离频率在不同稻区有显著差异。     

端粒酶的研究进展

端粒酶是由RNA模板和蛋白质催化亚基组成的核糖蛋白颗粒,它解决染色体的未端复制问题,归属于逆转录酶家族又和逆转录酶有一定的差别。端粒酶的缺失能在细胞和机体水平引起各种疾病的发生,且端粒的过度表达和细胞的永生化和癌变直接相关,其中端粒蛋白在此过程中可能起调控作用,端粒酶的结构和功能决定了它有广泛的应用前景。     

Estimation of Cellobiohydrolase I Activity by Numerical Differentiation of Dynamic Ultraviolet Spectroscopy

1,4-β-D-glucan cellobiohydrolase I (CBH I),p-nitrophenyl β-D-cellobioside,p-nitrophenol andcellobiose show distinct ultraviolet spectra,allowing the design of an assay to track the dynamic process ofp-nitrophenyl β-D-cellobioside hydrolysis by CBH I.Based on the linear relationship between p-nitrophenolformation in the hydrolysate and its first derivative absorption Curve of AUC340_400_(nm)(area under the curve),a new sensitive assay for the determination of CBH I activity was developed.The dynamic parameters ofcatalysis reaction,such as Vm and k_(cat),can all be derived from this result.The influence of β-glucosidase andendoglucanase in crude enzyme sample on the assay was discussed in detail.This approach is useful foraccurate determination of the activity of CBHs.     

Study of microbial community structures in UASB sludge treating municipal wastewater by denaturing gradient gel electrophoresis of 16S rDNA

The structures of microbial communities in lab-scale upflow anaerobic sludge blanket (UASB) reactors for treating municipal wastewater with different ratios of COD soluble/ COD total were studied using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes. The microbial structure of the inoculum sludge obtained from a full-scale UASB reactor of treating potato processing wastewater was compared with the structures of sludges collected from three lab-scale UASB reactors after eight months feeding with raw municipal wastewater, with CEPS (chemically enhanced primary sedimentation) pretreated municipal wastewater, and with a synthetic municipal sewage, respectively. Computer-aided numerical analysis of the DGGE fingerprints showed that the bacterial community underwent major changes. The sludges for treating raw and CEPS pretreated wastewater had very similar bacterial and archaeal communities (82% and 96% similarity) but were different from that for treating the synthetic sewage. Hence, despite     

新城病毒浓缩法的初步报告

近年来国内农业科举研究机关进行新城病疫苗的制造,一般制造的疫苗为鸡胚富尔马林疫苗或鸡胚化毒力减弱病毒疫苗(粟寿初与马闻天,1950;李善达,1951;杜念兴,1954)。作者在过去报告中(高尚荫,1952)曾指出在其它条件固定的情况下,有机     

纺织基人造血管几何特征表征的实验研究

人造血管的壁厚和松弛内径是其主要的几何特征参数。本文中首先依据国际标准ISO 7198的要求设计了测试人造血管松弛内径的测试定规,并对人造血管样品进行了测试。然后对人造血管壁厚的测试方法进行了初步探讨。     

SHP2及其在乳腺癌发生发展中的作用

SHP2是一种非受体型蛋白酪氨酸磷酸酶,其介导的信号转导异常与多种疾病包括肿瘤的发生和发展密切相关,对SHP2的深入研究有助于对其作用机制的阐明以及潜在药物学靶点的发现。本文简要介绍了SHP2的结构、功能及其介导的Ras/ERK信号通路,并着重阐述了SHP2与乳腺癌发展的关系。     

ASK1 physically interacts with COI1 and is required for male fertility in Arabidopsis

Jasmonates are a new class of plant hormones that play important roles in plant development and plant defense. The COI1 gene was previously shown to be required for jasmonate-regulated plant fertility and defense. We demonstrated for the first time that COI1 interacts with the Arabidopsis SKP1-LIKE1 (ASK1) to form a complex that is required for jasmonate action in planta. Functional analysis by antisense strategy showed that ASK1 is involved in male fertility.