全文获取类型
收费全文 | 2930篇 |
免费 | 157篇 |
专业分类
3087篇 |
出版年
2023年 | 9篇 |
2022年 | 18篇 |
2021年 | 41篇 |
2020年 | 19篇 |
2019年 | 30篇 |
2018年 | 51篇 |
2017年 | 27篇 |
2016年 | 55篇 |
2015年 | 85篇 |
2014年 | 110篇 |
2013年 | 185篇 |
2012年 | 232篇 |
2011年 | 213篇 |
2010年 | 133篇 |
2009年 | 110篇 |
2008年 | 204篇 |
2007年 | 209篇 |
2006年 | 188篇 |
2005年 | 174篇 |
2004年 | 205篇 |
2003年 | 172篇 |
2002年 | 200篇 |
2001年 | 19篇 |
2000年 | 24篇 |
1999年 | 25篇 |
1998年 | 31篇 |
1997年 | 33篇 |
1996年 | 38篇 |
1995年 | 29篇 |
1994年 | 30篇 |
1993年 | 26篇 |
1992年 | 16篇 |
1991年 | 21篇 |
1990年 | 12篇 |
1989年 | 13篇 |
1988年 | 13篇 |
1987年 | 14篇 |
1986年 | 7篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 10篇 |
1982年 | 9篇 |
1981年 | 9篇 |
1980年 | 10篇 |
1979年 | 6篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1970年 | 2篇 |
1969年 | 2篇 |
排序方式: 共有3087条查询结果,搜索用时 15 毫秒
1.
Kazuhiro Nakaya 《Ichthyological Research》1988,35(2):133-141
Additional specimens of the rareApristurus herklotsi are reported, and the characteristic features of this species are discussed.A. herklotsi is concluded to be a distinct species, having a very long snout, a narrow distance between the nostrils, a long caudal fin,
a short abdomen, numerous teeth on both jaws, and a low number of monospondylous vertebrae.A. herklotsi appears to be mature at about 44 cm in total length. 相似文献
2.
Mark T Uhlik Amy N Abell Bruce D Cuevas Kazuhiro Nakamura Gary L Johnson 《Biochimie et biologie cellulaire》2004,82(6):658-663
Mitogen-activated protein kinase (MAPK) pathways are activated by a plethora of stimuli. The literature is filled with papers describing the activation of different MAPKs by almost any stimulus or insult imaginable to cells. In this review, we use signal transduction wiring diagrams to illustrate putative upstream regulators for the MAPK kinase kinases, MEKK1, 2, and 3. Targeted gene disruption of MEKK1, 2, or 3 defined phenotypes for each MEKK associated with loss of specific MAPK regulation. Genetic analysis of MEKK function clearly defines specific components of the wiring diagram that require MEKK1, 2, or 3 for physiological responses. We propose that signal transduction network wiring diagrams are valuable tools for hypothesis building and filtering physiologically relevant phenotypic responses from less connected protein relations in the regulation of MAPK pathways. 相似文献
3.
Summary From a detailed complementation analysis of the region II che mutants of Salmonella typhimurium, we have located five che genes, cheA, cheW, cheR, cheB, and cheY. We have shown that corrections are required in the previous assignment of the mutations in four strains: both SL2514 and SL2515 which have been reported to be cheY mutants are cheR mutants, SL2539 is not a cheA but a cheW mutant, and ST171 which has been reported to be a cheZ mutant is a double mutant with defects in both cheA and cheB. Since ST171 is the only cheZ mutant so far isolated, the idea that the cheZ gene might play an essential role in chemotaxis in S. typhimurium as in Escherichia coli has lost its experimental basis. Furthermore, a number of deletion mutants in region II resulting from the excision of Tn10 have been isolated and analysed. From these experiments, we propose that the gene order in region II is flaK-flaE-motA-motB-cheA-cheW-cheR-cheB-cheY-flaM-flaC, which is identical with that in E. coli. 相似文献
4.
Summary The actin-activated ATPase activityPhysarum myosin was shown to be inhibited of M levels of Ca2+. To determine if Ca2+ regulates ATP-dependent movement ofPhysarum myosin on actin, latex beads coated withPhysarum myosin were introduced intoChara cells by intracellular perfusion. In perfusion solution containing EGTA, the beads moved along the parallel arrays ofChara actin filaments at a rate of 1.0–1.8 m/sec; however, in perfusion solution containing Ca2+, the rate reduced to 0.0–0.7 m/sec. The movement of beads coated with scallop myosin, whose actin-activated ATPase activity is activated by Ca2+, was observed only in the perfusion solution containing Ca2+, indicating that myosin is responsible for the inhibitory effect of Ca2+ onPhysarum myosin movement. The involvement of this myosin-linked regulation in the inhibitory effect of Ca2+ on the cytoplasmic streaming observed inChara internodal cell andPhysarum plasmodium was discussed.Abbreviations ATP
adenosine 5-triphosphate
- DTT
dithiothreitol
- EDTA
ethylenediaminetetraacetic acid
- EGTA
ethyleneglycolbis(-aminoethylether) N,N,N,N-tetraacetic acid
- PIPES
piperazine-N,N-bis(2-ethanesulfonic acid) 相似文献
5.
6.
Satoshi Nakamura Kunimasa Koga 《Biochemical and biophysical research communications》1977,78(2):806-810
By the method of differential scanning calorimetry, it was found that thermal stability of glucose oxidase was dependent on its redox states. The oxidized form showed an apparent denaturation temperature at 76°C and the denaturation enthalpy was approximately 865 kcal/mol. On reduction of the enzyme, the denaturation temperature increased by about 10°, but no significant change was seen in the denaturation enthalpy. The activation energies of the denaturation of the oxidized and the reduced enzymes were about 89 and 103 kcal/mol, respectively. These results may imply conformational changes in the catalytic turnover of this enzyme. 相似文献
7.
Yoshikazu Arai Jun Ohgane Shuh‐hei Fujishiro Kazuaki Nakano Hitomi Matsunari Masahito Watanabe Kazuhiro Umeyama Dai Azuma Naomi Uchida Nozomu Sakamoto Tomohiro Makino Shintaro Yagi Kunio Shiota Yutaka Hanazono Hiroshi Nagashima 《Genesis (New York, N.Y. : 2000)》2013,51(11):763-776
Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)‐specific hypomethylated loci (EShypo‐T‐DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso‐4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso‐4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso‐622‐14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum‐free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763–776. © 2013 Wiley Periodicals, Inc. 相似文献
8.
9.
10.