全文获取类型
收费全文 | 62271篇 |
免费 | 5982篇 |
国内免费 | 22篇 |
专业分类
68275篇 |
出版年
2023年 | 245篇 |
2022年 | 528篇 |
2021年 | 1034篇 |
2020年 | 646篇 |
2019年 | 828篇 |
2018年 | 1013篇 |
2017年 | 885篇 |
2016年 | 1519篇 |
2015年 | 2532篇 |
2014年 | 2812篇 |
2013年 | 3265篇 |
2012年 | 4348篇 |
2011年 | 4220篇 |
2010年 | 2665篇 |
2009年 | 2427篇 |
2008年 | 3474篇 |
2007年 | 3533篇 |
2006年 | 3338篇 |
2005年 | 3186篇 |
2004年 | 3114篇 |
2003年 | 2895篇 |
2002年 | 2788篇 |
2001年 | 967篇 |
2000年 | 803篇 |
1999年 | 885篇 |
1998年 | 886篇 |
1997年 | 621篇 |
1996年 | 540篇 |
1995年 | 503篇 |
1994年 | 524篇 |
1993年 | 534篇 |
1992年 | 687篇 |
1991年 | 557篇 |
1990年 | 544篇 |
1989年 | 554篇 |
1988年 | 482篇 |
1987年 | 457篇 |
1986年 | 449篇 |
1985年 | 444篇 |
1984年 | 467篇 |
1983年 | 431篇 |
1982年 | 444篇 |
1981年 | 415篇 |
1980年 | 416篇 |
1979年 | 344篇 |
1978年 | 329篇 |
1977年 | 303篇 |
1976年 | 279篇 |
1974年 | 255篇 |
1973年 | 228篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
Formation of rings from Drosophila DNA fragments 总被引:1,自引:0,他引:1
3.
Thirteen healthy and fit men [age = 27 +/- 8 (SD) yr, height = 177 +/- 5 cm, mass = 75 +/- 7 kg, body fat = 14 +/- 5%, maximal O2 consumption = 51 +/- 4 ml. kg-1. min-1] participated in an experiment designed to test their thermoregulatory response to a challenging cold exposure after 5 h of demanding mixed exercise during which only water was consumed. Subjects expended 7,314 +/- 741 kJ on cycling, rowing, and treadmill-walking machines, performed 8,403 +/- 1,401 kg. m of mechanical work during resistance exercises, and completed 120 inclined sit-ups. Subjects then assumed a seated position in a 10 degrees C air environment while wearing shorts, T-shirt, rain hat, and neoprene gloves and boots. After 30 min the subjects were showered continuously with cold water ( approximately 920 ml/min at 10 degrees C) on their backs accompanied by a 6 km/h wind for up to 4 h. Blood samples were taken from the nondominant arm every 30 min during the exposure and assayed for energy metabolites, hormones, indexes of hydration, and neurotransmitters. Counterbalanced control trials without prior exercise were also conducted. Blood insulin was higher during the control trial, whereas values of glycerol, nonesterified fatty acids, beta-hydroxybutyrate, lactate, cortisol, free triiodothyronine, and thyroxine were lower. Three subjects lasted the maximum duration of 4.5 h for control and fatigue trials, with final rectal temperatures of 36.43 +/- 0.21 and 36.08 +/- 0.49 degrees C, respectively. Overall, the duration of 172 +/- 68 (SD) min for the fatigue trial was not significantly different from that of the control trial (197 +/- 72 min) and, therefore, was not affected by the preexposure exercise. Although duration was positively correlated to body fatness and shivering intensity, the latter was not correlated to any physical characteristic or the fitness level of the individual. 相似文献
4.
5.
6.
Thomas J Hughes 《BMJ (Clinical research ed.)》1980,281(6239):562-563
7.
8.
9.
Yongbo Hu Erin Webb Jasbir Singh Barry A Morgan James A Gainor Thomas D Gordon Teruna J Siahaan 《The Journal of biological chemistry》2002,277(10):8366-8371
The molecular basis of the substrate specificity of Clostridium histolyticum beta-collagenase was investigated using a combinatorial method. An immobilized positional peptide library, which contains 24,000 sequences, was constructed with a 7-hydroxycoumarin-4-propanoyl (Cop) fluorescent group attached at the N terminus of each sequence. This immobilized peptide library was incubated with C. histolyticum beta-collagenase, releasing fluorogenic fragments in the solution phase. The relative substrate specificity (k(cat)/K(m)) for each member of the library was determined by measuring fluorescence intensity in the solution phase. Edman sequencing was used to assign structure to subsites of active substrate mixtures. Collectively, the substrate preference for subsites (P(3)-P(4)') of C. histolyticum beta-collagenase was determined. The last position on the C-terminal side in which the identity of the amino acids affects the activity of the enzyme is P(4)', and an aromatic side chain is preferred in this position. The optimal P(1)'-P(3)' extended substrate sequence is P(1)'-Gly/Ala, P(2)'-Pro/Xaa, and P(3)'-Lys/Arg/Pro/Thr/Ser. The Cop group in either the P(2) or P(3) position is required for a high substrate activity with C. histolyticum beta-collagenase. S(2) and S(3) sites of the protease play a dominant role in fixing the substrate specificity. The immobilized peptide library proved to be a powerful approach for assessing the substrate specificity of C. histolyticum beta-collagenase, so it may be applied to the study of other proteases of interest. 相似文献
10.
Raj K. Singh Mary F. Ruh Thomas S. Ruh 《Biochimica et Biophysica Acta (BBA)/General Subjects》1984,800(1):33-40
In the present study we investigated the binding characteristics of estrogen and antiestrogen-receptor complexes to rabbit uterine chromatin. Activated or nonactivated estrogen receptors were partially purified by DEAE-cellulose chromatography using low (1 mM) or high (10 mM) concentrations of sodium molybdate. Activated [3H]estradiol-receptor complexes showed enhanced binding to chromatin acceptor sites unmasked by 1 M, 4 M and 6 M guanidine hydrochloride. We also examined the chromatin-binding characteristics of the estrogen receptors when bound by the high-affinity triphenylethylene antiestrogen, H1285. The acceptor site activity for the [3H]H1285-receptor complexes was markedly decreased at sites unmasked by 4 M and 6 M guanidine hydrochloride. Further, the nonactivated receptor complexes showed very low binding to deproteinized chromatin. The estrogen-receptor chromatin-acceptor sites were tissue specific and saturable. These chromatin acceptor sites differ in their affinity and capacity (number of binding sites per cell) for the estrogen- and antiestrogen-receptor complexes. Thus, we suggest that the differences in the physiological and physicochemical properties of estrogens and antiestrogens may be related to their differential interaction with uterine chromatin subfractions. 相似文献