排序方式: 共有2条查询结果,搜索用时 0 毫秒
1
1.
DUREJA INAM; GUHA-MUKHERJEE SIPRA; PRASAD RAJENDRA 《Journal of experimental botany》1984,35(7):1022-1031
The uptake of L-leucine and glycine into pea protoplasts wasstudied under various conditions. The uptake of both L-leucineand glycine was pH dependent with the optimal pH being 4.0 and5.0 for L-leucine and glycine, respectively. A kinetic studyof L-leucine uptake showed that uptake is multiphasic; Km valuesof different phases were 1.1 mol m3, 33.3 mol m3and 100 mol m3. A similar analysis for glycine at a concentrationrange of 0.110 mol m3 also showed a multiphasictransport system for it. The uptake of L-leucine at lower concentrations(between0.12.0 mol m3) was energy dependent, since arsenate,azide, dinitrophenol and iodoacetate inhibited the uptake. However,the uptake of L-leucine was not inhibited by ouabain at anyconcentration of L-leucine employed. The uptake of glycine wasnot inhibited by any of these inhibitors suggesting that glycineuptake was not mediated by an active process. Key words: Pea protoplast, L-Leucine, Glycine transport, Active transport, Mediated transport 相似文献
2.
DUREJA INAM; GUHA-MUKHERJEE SIPRA; PRASAD RAJENDRA 《Journal of experimental botany》1986,37(4):549-555
Dureja, I., Guha-Mukherjee, S. and Prasad, R. 1986. Mechanismof L-lysine transport by pea protoplasts.J. exp. Bot.37: 549555. L-Lysine uptake was studied in pea protoplasts to characterizethe transport process. The uptake was pH dependent with optimumat pH 5?8. A kinetic analysis of uptake showed that L-lyslneuptake was biphasic. The respiratory inhibitors, sodium arsenate,azide, iodoacetate and 2, 4, dinitrophenol, inhibited the uptakeof L-lysine at a final concentration of 0?1 mol m3 suggestingit to be mediated in part by an active process. Competitiveinhibition of L-lysine uptake by only L-arglnine and of L-leucineand glycine uptake by several amino acids indicated that L-lysineuptake occurs via a specific system whereas the uptake of L-leucineand glycine was mediated through a relatively non-specific permease. Key words: Pea protoplasts, L-lysine transport, active transport, specific system 相似文献
1