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Patil Basavaprabhu L. Raghu Rajanna Dangwal Meenakshi Byregowda M. Voloudakis Andreas 《Journal of plant biochemistry and biotechnology.》2021,30(2):400-405
Journal of Plant Biochemistry and Biotechnology - Pigeonpea sterility mosaic emaraviruses (PPSMVs) cause sterility mosaic disease in pigeonpea which significantly reduce the crop yield. Currently... 相似文献
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Patil Basavaprabhu L. Raghu Rajanna Dangwal Meenakshi Byregowda M. Voloudakis Andreas 《Journal of plant biochemistry and biotechnology.》2022,31(1):226-226
Journal of Plant Biochemistry and Biotechnology - Unfortunately, the title of the article was incorrectly published in the original publication. The correct title is updated. 相似文献
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Rob W Briddon Basavaprabhu L Patil Basavaraj Bagewadi Muhammad Shah Nawaz-ul-Rehman Claude M Fauquet 《BMC evolutionary biology》2010,10(1):97
Background
Viruses of the genus Begomovirus (family Geminiviridae) have genomes consisting of either one or two genomic components. The component of bipartite begomoviruses known as DNA-A is homologous to the genomes of all geminiviruses and encodes proteins required for replication, control of gene expression, overcoming host defenses, encapsidation and insect transmission. The second component, referred to as DNA-B, encodes two proteins with functions in intra- and intercellular movement in host plants. The origin of the DNA-B component remains unclear. The study described here was initiated to investigate the relationship between the DNA-A and DNA-B components of bipartite begomoviruses with a view to unraveling their evolutionary histories and providing information on the possible origin of the DNA-B component. 相似文献5.
Basavaprabhu L. Patil Deepika Arora 《Journal of plant biochemistry and biotechnology.》2018,27(4):382-392
High throughput sequencing technologies, supported by bioinformatics tools are employed to retrieve small RNA sequence information derived from the nucleic acids of plant infecting viruses. In addition to characterization of the small RNAs to understand the biology of the virus, the small RNA sequence can be assembled to reconstitute viral genome sequence. For the first time the semiconductor based Ion Proton sequencing technology is used to sequence the small RNAs from pigeonpea (Cajanus cajan) plants infected by two distinct viruses with RNA and DNA as their genomes. The reconstitution of the viral genome sequence revealed that the pigeonpea plant from Kalaburagi (erstwhile Gulbarga, Karnataka state) was infected by an emaravirus species Pigeonpea sterility mosaic emaravirus 1 (PPSMV-1) and another plant from New Delhi was infected by a begomovirus species Mungbean yellow mosaic India virus (MYMIV). Characterization and comparison of small RNA sequences derived from both the viruses showed vast differences in their pattern of accumulation and their size classes. In the case of PPSMV-1, the 21 nt sized siRNAs accumulated at far greater levels followed by 22 and 24 nt siRNAs. Whereas in MYMIV, the proportion of accumulation of each size class of siRNAs was similar. Further the distribution of small RNAs across the genomes of PPSMV-1 and MYMIV was mapped and the density of small RNA accumulation showed a positive correlation with the GC content of viral sequence. 相似文献
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RNAi-mediated resistance to diverse isolates belonging to two virus species involved in Cassava brown streak disease 总被引:1,自引:0,他引:1
Patil BL Ogwok E Wagaba H Mohammed IU Yadav JS Bagewadi B Taylor NJ Kreuze JF Maruthi MN Alicai T Fauquet CM 《Molecular Plant Pathology》2011,12(1):31-41
Cassava brown streak disease (CBSD) is emerging as one of the most important viral diseases of cassava (Manihot esculenta) and is considered today as the biggest threat to cassava cultivation in East Africa. The disease is caused by isolates of at least two phylogenetically distinct species of single-stranded RNA viruses belonging to the family Potyviridae, genus Ipomovirus. The two species are present predominantly in the coastal lowland [Cassava brown streak virus (CBSV); Tanzania and Mozambique] and highland [Cassava brown streak Uganda virus (CBSUV); Lake Victoria Basin, Uganda, Kenya and Malawi] in East Africa. In this study, we demonstrate that CBSD can be efficiently controlled using RNA interference (RNAi). Three RNAi constructs targeting the highland species were generated, consisting of the full-length (FL; 894 nucleotides), 397-nucleotide N-terminal and 491-nucleotide C-terminal portions of the coat protein (CP) gene of a Ugandan isolate of CBSUV (CBSUV-[UG:Nam:04]), and expressed constitutively in Nicotiana benthamiana. After challenge with CBSUV-[UG:Nam:04], plants homozygous for FL-CP showed the highest resistance, followed by the N-terminal and C-terminal lines with similar resistance. In the case of FL, approximately 85% of the transgenic plant lines produced were completely resistant. Some transgenic lines were also challenged with six distinct isolates representing both species: CBSV and CBSUV. In addition to nearly complete resistance to the homologous virus, two FL plant lines showed 100% resistance and two C-terminal lines expressed 50-100% resistance, whereas the N-terminal lines succumbed to the nonhomologous CBSV isolates. Northern blotting revealed a positive correlation between the level of transgene-specific small interfering RNAs detected in transgenic plants and the level of virus resistance. This is the first demonstration of RNAi-mediated resistance to CBSD and protection across very distant isolates (more than 25% in nucleotide sequence) belonging to two different species: Cassava brown streak virus and Cassava brown streak Uganda virus. 相似文献
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Thien Van Truong Mihir Ghosh Basavaprabhu Hosamani Thekke V. Baiju Gunasekaran Dhandapani Ellen Wachtel Ellina Kesselman Dganit Danino Mordechai Sheves Irishi N.N. Namboothiri Guy Patchornik 《Journal of peptide science》2019,25(6)
Abstract We report the first demonstration of nonionic detergent micelle conjugation and phase separation using purpose‐synthesized, peptide amphiphiles, C10‐(Asp)5 and C10‐(Lys)5. Clustering is achieved in two different ways. Micelles containing the negatively charged peptide amphiphile C10‐(Asp)5 are conjugated (a) via a water‐soluble, penta‐Lys mediator or (b) to micelles containing the C10‐(Lys)5 peptide amphiphile. Both routes lead to phase separation in the form of oil‐rich globules visible in the light microscope. The hydrophobic nature of these regions leads to spontaneous partitioning of hydrophobic dyes into globules that were found to be stable for weeks to months. Extension of the conjugation mechanism to micelles containing a recently discovered, light‐driven proton pump King Sejong 1‐2 (KS1‐2) demonstrates that a membrane protein may be concentrated using peptide amphiphiles while preserving its native conformation as determined by characteristic UV absorption. The potential utility of these peptide amphiphiles for biophysical and biomedical applications is discussed. 相似文献
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Imran Amin Basavaprabhu L Patil Rob W Briddon Shahid Mansoor Claude M Fauquet 《Virology journal》2011,8(1):1-24